ρ-Aminobenzamidine Agarose primary application is for the removal and /or purification of trypsin-like proteases.ρ-aminobenzamidine (PAB) is a synthetic inhibitor of trypsin-like proteases and has been covalently coupled to 6% crosslinked agarose. ρ-aminobenzamidine agaroses have…

Lectins are proteins or glycoproteins that have at least one noncatalytic domain that binds specifically and reversibly to monosaccharides or oligosaccharides. Lectins are important as they are found in applications in science, medicine, agriculture, pharmaceutical companies, biotechnology and…

G-Biosciences Immobilized Amino Acids are prepared by coupling amino acid to preactivated 6% cross-linked agarose. The covalent coupling to agarose (resin) is achieved by cross-linking amino group of the amino acid to the preactivated resin. Features: Amino acid coupled to 6%…

An important tool in research is the generation of peptide and protein affinity columns for the purification of antibodies and for the discovery of important interacting proteins and cofactors. G-Biosciences offersa complete kit for the coupling of peptides and proteins to agarose through their…

G-Biosciences Immobilized D-Galactose is for the purification of lectins, galactosidases and other galactose-, N-acetylgalactosamine- or carbohydrate binding molecules. Specific applications include the purification of galactosidases, C-type lectins, enterotoxins and cholera toxin. The resin…

Glutathione Resin is designed for the affinity purification of proteins with a glutathione S-transferase (GST) tag. The resin consists of reduced glutathione (GSH) covalently coupled to 6% cross-linked agarose, via a 10-carbon spacer arm. The resin has a binding capacity of >40mg GST/ml resin.…

Immobilized Metal Ion Affinity Chromatography (IMAC), developed by Porath (1975), is based on the interaction of certain protein residues (histidines, cysteines, and to some extent tryptophans) with cations of transition metals. The Co-NTA Resin is specifically designed for the purification of…

Sepharose 6B is a well-proven agarose gel filtration base matrix and is frequently used for coupling affinity ligands to the matrix. The matrix is not pre-activated and the user performs all steps in coupling. 6% Agarose gel filtration media. Proven base matrix for coupling affinity ligands 6%…

NHS-activated Sepharose 4 Fast Flow is a pre-activated agarose matrix that increases the choice of coupling chemistries available. Suitable for coupling amino-containing smaller proteins and peptides. NHS pre-activated medium for coupling of small amino-containing proteins and peptides in…

Immobilized Metal Ion Affinity Chromatography (IMAC), developed by Porath (1975), is based on the interaction of certain protein residues (histidines, cysteines, and to some extent tryptophans) with cations of transition metals.G-Sep™NI NTAAgarose Fast Flow is specifically designed for the…

Sepharose 4B is a well-proven agarose gel filtration base matrix and is frequently used for coupling affinity ligands to the matrix. The matrix is not pre-activated and the user performs all steps in coupling. 4% Agarose gel filtration media. Proven base matrix for coupling affinity ligands 4%…

Immobilized Metal Ion Affinity Chromatography (IMAC), developed by Porath et al (1), is based on the interaction of certain protein residues (histidines, cysteines, and to some extent tryptophans) with cations of transition metals. The Copper Chelating Resin is specifically designed for the…

The Carbohydrate Coupling Resin is designed for the simple and efficient coupling of glycoproteins to a solid agarose support through oxidized sugar groups. The coupling is through the hydrazide group that is immobilized to agarose beads. The resin is ideal for immobilized polyclonal antibodies as…

lgG Sepharose 6 Fast Flow, from Cytiva , is based on the rigid Sepharose 6 Fast Flow matrix, with human IgG covalently coupled to it. Medium allows high flow rates to be used for rapid and convenient single step purification of protein IgG coupled to the highly cross-linked 6% agarose matrix…

Immobilized Protein A/G consists of recombinant protein A/G ligand covalently immobilized onto 6% highly cross-linked agarose.The dynamic binding capacity will vary depending on several factors such as target antibody, flow rate etc. Protein A/G binds well to IgG subclasses but does not bind…

Immobilized Metal Ion Affinity Chromatography (IMAC), developed by Porath (1), is based on the interaction of certain protein residues (histidines, cysteines, and to some extent tryptophans) with cations of transition metals. The Zinc Chelating Resin is specifically designed for the purification of…

G-Biosciences Immobilized Heparin is a ready-to-use purification resin for a wide range of proteins.The resin consists of 6% cross-linked agarose covalently coupled to heparin through amide bonds.The coupling chemistry used generates a highly stable purification resin that is stable most commonly…

CNBr-activated Sepharose 4 Fast Flow is a well established, pre-activated chromatography medium for coupling of large amino-containing ligands. CNBr-activated BioProcess medium designed for coupling of large amino-containing ligands. Easy, rapid and efficient coupling This resin allows for…

Sepharose CL-4B is a well-proven cross-linked agarose gel filtration base matrix and is frequently used for coupling affinity ligands to the matrix. The matrix is not pre-activated and the user performs all steps in coupling. 4% cross-linked agarose gel filtration media. Proven base matrix for…

Immobilized Metal Ion Affinity Chromatography (IMAC), developed by Porath (1975), is based on the interaction of certain protein residues (histidines, cysteines, and to some extent tryptophans) with cations of transition metals.G-Sep™ Co IDA Agarose Fast Flow is specifically designed for the…

Thiophilic adsorption or thiophilic chromatography is a routinely used technique for the low cost, simple purification of immunoglobulins. Thiophilic adsorption was first developed by Porath et al 1 in 1984 and is a group specific, salt-dependent purification technique that has distinct affinity…

MabSelect Xtra, with extra high dynamic binding capacity, is a member of the MabSelect family. Developed to meet the demands of increasing levels of expression in monoclonal antibody feedstocks Higher dynamic binding capacity than currently available Protein A chromatography media. Improved…

Gelatin Sepharose 4B affinity resin with immobilized gelatin is designed for the purification or depletion of fibronectin as gelatin binds to fibronectin with high specificity. Gelatin Sepharose 4B is bovine gelatin coupled to Sepharose 4B by the cyanogen bromide method Gelatin Sepharose 4B is…

MabSelect SuRe is a protein A affinity medium for capturing MAbs at process scale. MabSelect SuRe has an alkali-tolerant rProtein A ligand that allows the use of rigorous and cost-effective CIP and sanitization protocols based on 0.1 to 0.5 M NaOH. Further, the ligand is protease stable leading to…

Immobilized protein G is for binding the constant domains of immunoglobulin (Ig) molecules (see Figure 1). It is ideal for the easy one-step purification of classes, subclasses and fragments of immunoglobulins for biological fluids and cell culture media. Protein G is a modified form of…

Immobilized Metal Ion Affinity Chromatography (IMAC), developed by Porath (1975), is based on the interaction of certain protein residues (histidines, cysteines, and to some extent tryptophans) with cations of transition metals. The Ni-NTA Resin is specifically designed for the purification of…

Immobilized Metal Ion Affinity Chromatography (IMAC), developed by Porath (1), is based on the interaction of certain protein residues (histidines, cysteines, and to some extent tryptophans) with cations of transition metals. The Cobalt Chelating Resin is specifically designed for the…

Immunoprepitation Kit: Immunoprecipitation (IP) allows purification of a protein from a complex mixture of proteinsusing a specific antibody and a matrix that binds to the antibody. The matrices commonly used are agarose or sepharose bound Protein A or protein A/G PLUS. IP followed by…

Ficin (or Ficain) is a cysteine protease enzyme (EC 3.4.22.3) isolated from fig latex is that has the endopeptidase activity to cleave immunoglobulin G molecules in the hinge reason.Ficin is typically used to cleave mouse IgG₁ as this are difficult to cleave with papain and pepsin .In…

Capto Blue is an affinity medium for the capture of albumin, purification of enzymes, and recombinant proteins at laboratory and process scales. Excellent chemical stability ensures tolerance of harsh solvents used in repeated cleaning-in-place and sanitization procedures. Capto Blue can be…

Immunoprepitation Kit: Immunoprecipitation (IP) allows purification of a protein from a complex mixture of proteinsusing a specific antibody and a matrix that binds to the antibody. The matrices commonly used are agarose or sepharose bound Protein A or protein A/G PLUS. IP followed by…

Biotin, a 244Da vitamin (Vitamin H) molecule, exhibits an extraordinary binding affinity for avidin (K_a=10 15 M -1 ). Biotin and avidin interaction is rapid and once the bond is established it can survive up to 3M guanidine-hydrochloride and extremes of pH. Biotin-avidin bonds can only…