Affinity Other Media

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Affinity Other Media

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  • Epoxy-Activated Agarose is preactivated resin used for affinity chromatography. Epoxy-Activated Agarose comprise of high density epoxy groups that form covalent bonds with ligand s containing amine groups, thiol or hydroxyl groups. Epoxide-Acivated Agarose is prepared by immobilization of…

  • G-Biosciences CDI Amine Reactive Agarose consists of 6% cross-linked agarose activated with CDI (1,1’-carbonyl diimidazole) to form reactive imidazole carbamates. The activation of the resin occurs in solvent and to maintain its activity the resin is supplied in acetone to prevent…

  • Ni Sepharose 6 Fast Flow is a BioProcess medium that combines the advantages of Ni?? for purification of histidine tagged proteins, with the well industrially established properties of the Sepharose Fast Flow platform. Designed for scaling up purification of histidine-tagged proteins High…

  • Streptavidin Sepharose High Performance is an affinity chromatography medium designed for fast and reliable, high resolution purification of biotinylated biomolecules. Streptavidin immobilized to Sepharose High Performance for purification of biotinylated compounds Extremely useful for…

  • Concanavalin A (Con A) Agarose consists of Con A coupled to 4% agarose by the cyanogen bromide method. Con A is a tetrameric metalloprotein lectin isolated from Canavalia ensiformis (jack bean). Con A is used for the purification of glycoproteins, polysaccharides and glycolipids as it binds…

  • Ni-NTA Magnetic Beads

    G-Biosciences

    G-Biosciences’ Ni-NTA Magnetic Beads are 3µm beads designed for the rapid purification of X His-tagged proteins. Ni-NTA Magnetic Beads have nitrilotriacetic acid (NTA) groups with charged nickel covalently bound to the surface dextran of the beads.Due to the high affinity, Ni-NTA…

  • Sepharose 6B

    Cytiva

    Sepharose 6B is a well-proven agarose gel filtration base matrix and is frequently used for coupling affinity ligands to the matrix. The matrix is not pre-activated and the user performs all steps in coupling. 6% Agarose gel filtration media. Proven base matrix for coupling affinity ligands 6%…

  • Immobilized Reductant

    G-Biosciences

    Reducing agents are used in the reduction of disulfide bonds of proteins and peptides. It is often necessary to remove the reducing agents from the protein/peptide solutions to prevent them from interfering with subsequent procedures. However, for small proteins, and particularly peptides, it is…

  • Immobilized Protein A/G consists of recombinant protein A/G ligand covalently immobilized onto 6% highly cross-linked agarose.The dynamic binding capacity will vary depending on several factors such as target antibody, flow rate etc. Protein A/G binds well to IgG subclasses but does not bind…

  • lgG Sepharose 6 Fast Flow, from Cytiva , is based on the rigid Sepharose 6 Fast Flow matrix, with human IgG covalently coupled to it. Medium allows high flow rates to be used for rapid and convenient single step purification of protein IgG coupled to the highly cross-linked 6% agarose matrix…

  • Heparin Sepharose 6 Fast Flow is a well established BioProcess affinity resin designed for capture or intermediate purification steps. This resin is extensively used for industrial purification of several marketed plasma proteins. Industrial purification of antithrombin III, coagulation factors…

  • Glutathione Resin

    G-Biosciences

    Glutathione Resin is designed for the affinity purification of proteins with a glutathione S-transferase (GST) tag. The resin consists of reduced glutathione (GSH) covalently coupled to 6% cross-linked agarose, via a 10-carbon spacer arm. The resin has a binding capacity of >40mg GST/ml resin.…

  • Ni-NTA Resin

    G-Biosciences

    Immobilized Metal Ion Affinity Chromatography (IMAC), developed by Porath (1975), is based on the interaction of certain protein residues (histidines, cysteines, and to some extent tryptophans) with cations of transition metals. The Ni-NTA Resin is specifically designed for the purification of…

  • Immunoprepitation Kit: Immunoprecipitation (IP) allows purification of a protein from a complex mixture of proteinsusing a specific antibody and a matrix that binds to the antibody. The matrices commonly used are agarose or sepharose bound Protein A or protein A/G PLUS. IP followed by…

  • CNBr-activated Sepharose 4B is a pre-activated media used for coupling antibodies or other large proteins containing -NH? groups to the Sepharose media, by the cyanogen bromide method, without an intermediate spacer arm. For immobilizing antibodies or other large proteins containing -NH? groups…

  • NHS-activated Sepharose 4 Fast Flow is a pre-activated agarose matrix that increases the choice of coupling chemistries available. Suitable for coupling amino-containing smaller proteins and peptides. NHS pre-activated medium for coupling of small amino-containing proteins and peptides in…

  • Lectins are proteins or glycoproteins that have at least one noncatalytic domain that binds specifically and reversibly to monosaccharides or oligosaccharides. Lectins are important as they are found in applications in science, medicine, agriculture, pharmaceutical companies, biotechnology and…

  • Epoxy-activated Sepharose 6B is a pre-activated medium for immobilization of various ligands including sugars through coupling of hydroxy, amino or thiol groups on the ligand to Sepharose 6B via a 12-atom hydrophilic spacer arm Epoxy-activated Sepharose 6B can be used to couple sugars and other…

  • Immobilized Heparin

    G-Biosciences

    G-Biosciences Immobilized Heparin is a ready-to-use purification resin for a wide range of proteins.The resin consists of 6% cross-linked agarose covalently coupled to heparin through amide bonds.The coupling chemistry used generates a highly stable purification resin that is stable most commonly…

  • CNBr-Activated Agarose is pre-activated high capacity resinused for covalent coupling of antibodies and large protein molecules for affinity chromatography. Cyanogen bromide reacts with hydroxyl groups of resin to form cyanate esters which inturn reacts with primary amine ligand s to form…

  • MabSelect

    Cytiva

    MabSelect is a protein A affinity medium designed for capturing MAbs from large sample volumes. High-flow agarose matrix allows for high flow velocities at production scale enabling the processing of more than 10 000 L of feed in one working day. Oriented coupling of the ligand and optimized…

  • Sepharose CL-6B is a well-proven cross-linked agarose gel filtration base matrix and is frequently used for coupling affinity ligands to the matrix. The matrix is not pre-activated and the user performs all steps in coupling. 6% cross-linked agarose gel filtration media. Proven base matrix for…

  • Sepharose CL-4B is a well-proven cross-linked agarose gel filtration base matrix and is frequently used for coupling affinity ligands to the matrix. The matrix is not pre-activated and the user performs all steps in coupling. 4% cross-linked agarose gel filtration media. Proven base matrix for…

  • Immobilized Metal Ion Affinity Chromatography (IMAC), developed by Porath (1975), is based on the interaction of certain protein residues (histidines, cysteines, and to some extent tryptophans) with cations of transition metals.G-Sep™NI NTAAgarose Fast Flow is specifically designed for the…

  • Sepharose 4B

    Cytiva

    Sepharose 4B is a well-proven agarose gel filtration base matrix and is frequently used for coupling affinity ligands to the matrix. The matrix is not pre-activated and the user performs all steps in coupling. 4% Agarose gel filtration media. Proven base matrix for coupling affinity ligands 4%…

  • Nickel Chelating Resin

    G-Biosciences

    Immobilized metal affinity chromatography (IMAC) resin utilizing nickel (Ni 2+ ) for the purification of 6x histidine tagged proteins. This resin binds to six histidine residues (6x His), a common tag used in protein purification. The resin consists of iminodiacetate coupled to 6% cross-linked…

  • Boronate Resin

    G-Biosciences

    Our Boronate resin is designed for the isolation ofribonucleotide and oligonucleotide RNA. The resin consists of boronic acid covalently linked to a polyacrylamide support that offers simple isolation of small molecular weight compounds that have cis-diol groups. Mechanism : The boronic…

  • Zinc Chelating Resin

    G-Biosciences

    Immobilized Metal Ion Affinity Chromatography (IMAC), developed by Porath (1), is based on the interaction of certain protein residues (histidines, cysteines, and to some extent tryptophans) with cations of transition metals. The Zinc Chelating Resin is specifically designed for the purification of…

  • Amine Magnetic Beads

    G-Biosciences

    G-Biosciences’ Amine Magnetic Beads are 1µm, uniformmagnetic beads with an amine (-NH2) surface functional group. The magnetic beads consist of a single-crystal Fe3O4 sphere core and dextran coating layer. Through chemical modification of dextran, the…

  • Blue Sepharose 6 Fast Flow is Cibacron Blue 3G coupled to Sepharose 6 Fast Flow. This medium is well established as adsorbent for albumin and interferon at both laboratory and process scale Designed for large-scale purification of interferon and albumin. Also used for isolating groups of enzymes…

  • Thiophilic Resin

    G-Biosciences

    Thiophilic adsorption or thiophilic chromatography is a routinely used technique for the low cost, simple purification of immunoglobulins. Thiophilic adsorption was first developed by Porath et al 1 in 1984 and is a group specific, salt-dependent purification technique that has distinct affinity…

  • Immobilized Procainamide

    G-Biosciences

    Immobilized Procainamide is designed for affinity purification of butyrylcholinesterase (BuChE), which is also known as non-specific cholinesterase. Butyrylcholinesterase can be purified by batch adsorption on immobilized Procainamide followed by ion-exchange chromatography on DEAE-Sepharose…

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