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G-Biosciences

Concanavalin A (Con A) Agarose

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Concanavalin A (Con A) Agarose consists of Con A coupled to 4% agarose by the cyanogen bromide method. Con A is a tetrameric metalloprotein lectin isolated from Canavalia ensiformis (jack bean).

Con A is used for the purification of glycoproteins, polysaccharides and glycolipids as it binds molecules containing α-D-mannopyranosyl, α-D-glucopyranosyl and sterically related residues. Con A agarose has also be used in other application areas including purification of enzyme-antibody conjugates, purification of IgM and separation of membrane vesicles.

As stated above, Con A is a metalloprotein and to maintain its binding characteristics the presence of both Mn2+ and Ca2+ is essential. Each subunit of Con A utilizes one calcium and one manganese ion and these cations can be removed under acidic conditions abolishing the carbohydrate-binding activity.

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Features

  • Binds α-D-mannopyranosyl, α-D-glucopyranosyl and sterically related residues
  • Purification/ enrichment of of glycoproteins, polysaccharides and glycolipids
  • Ligand Density: 10-16mg Con A/ml resin
  • Capacity: 20-50mg thyroglobulin/ml resin
  • Bead structure: 4% agarose

Applications

  • Purification/ enrichment of of glycoproteins, polysaccharides and glycolipids

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Product Detail
Thomas No.
C752D12
Mfr. No.
786-208
Description
Concanavalin A (Con A) Agarose, 10 columns

list price/quantitytotal
$0.00
Thomas No.
C752D22
Mfr. No.
786-216
Description
Concanavalin A (Con A) Agarose, 5mL resin

list price/quantitytotal
$0.00
Thomas No.
C752D23
Mfr. No.
786-217
Description
Concanavalin A (Con A) Agarose, 25mL resin

list price/quantitytotal
$0.00
Thomas No.
C752D24
Mfr. No.
786-218
Description
Concanavalin A (Con A) Agarose, 100mL resin

list price/quantitytotal
$0.00
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Product is restricted and can only be purchased by customers with a web profile linked to a Thomas Business Account - Click here to login or create your web profile. If you do not have a Thomas Business Account, please Contact Us and someone will respond with details on how to apply for a business account with us.

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