CNBr-activated Sepharose 4 Fast Flow is a well established, pre-activated chromatography medium for coupling of large amino-containing ligands. CNBr-activated BioProcess medium designed for coupling of large amino-containing ligands. Easy, rapid and efficient coupling This resin allows for…

MabSelect is a protein A affinity medium designed for capturing MAbs from large sample volumes. High-flow agarose matrix allows for high flow velocities at production scale enabling the processing of more than 10 000 L of feed in one working day. Oriented coupling of the ligand and…

Biotin, a 244Da vitamin (Vitamin H) molecule, exhibits an extraordinary binding affinity for avidin (K_a=10 15 M -1 ). Biotin and avidin interaction is rapid and once the bond is established it can survive up to 3M guanidine-hydrochloride and extremes of pH. Biotin-avidin bonds can only…

Immobilized Metal Ion Affinity Chromatography (IMAC), developed by Porath (1975), is based on the interaction of certain protein residues (histidines, cysteines, and to some extent tryptophans) with cations of transition metals. The Ni-NTA Resin is specifically designed for the purification of…

Concanavalin A (Con A) Agarose consists of Con A coupled to 4% agarose by the cyanogen bromide method. Con A is a tetrameric metalloprotein lectin isolated from Canavalia ensiformis (jack bean). Con A is used for the purification of glycoproteins, polysaccharides and glycolipids as it binds…

G-Biosciences’ Ni-NTA Magnetic Beads are 3µm beads designed for the rapid purification of X His-tagged proteins. Ni-NTA Magnetic Beads have nitrilotriacetic acid (NTA) groups with charged nickel covalently bound to the surface dextran of the beads.Due to the high affinity, Ni-NTA…

Calmodulin Sepharose 4B affinity media for purification of proteins with affinity for calmodulin, a highly conserved regulatory protein involved in many cellular processes in eukaryotic cells. For single-step purification of native calmodulin-binding proteins Suitable for tandem affinity…

Sepharose 4B is a well-proven agarose gel filtration base matrix and is frequently used for coupling affinity ligands to the matrix. The matrix is not pre-activated and the user performs all steps in coupling. 4% Agarose gel filtration media. Proven base matrix for coupling affinity ligands 4%…

Immobilized Metal Ion Affinity Chromatography (IMAC), developed by Porath (1975), is based on the interaction of certain protein residues (histidines, cysteines, and to some extent tryptophans) with cations of transition metals.G-Sep™NI NTAAgarose Fast Flow is specifically designed for the…

Epoxy-activated Sepharose 6B is a pre-activated medium for immobilization of various ligands including sugars through coupling of hydroxy, amino or thiol groups on the ligand to Sepharose 6B via a 12-atom hydrophilic spacer arm Epoxy-activated Sepharose 6B can be used to couple sugars and other…

G-Biosciences CDI Amine Reactive Agarose consists of 6% cross-linked agarose activated with CDI (1,1’-carbonyl diimidazole) to form reactive imidazole carbamates. The activation of the resin occurs in solvent and to maintain its activity the resin is supplied in acetone to prevent…

Sepharose 6B is a well-proven agarose gel filtration base matrix and is frequently used for coupling affinity ligands to the matrix. The matrix is not pre-activated and the user performs all steps in coupling. 6% Agarose gel filtration media. Proven base matrix for coupling affinity ligands 6%…

Our Boronate resin is designed for the isolation ofribonucleotide and oligonucleotide RNA. The resin consists of boronic acid covalently linked to a polyacrylamide support that offers simple isolation of small molecular weight compounds that have cis-diol groups. Mechanism : The boronic…

Reducing agents are used in the reduction of disulfide bonds of proteins and peptides. It is often necessary to remove the reducing agents from the protein/peptide solutions to prevent them from interfering with subsequent procedures. However, for small proteins, and particularly peptides, it is…

Immobilized Metal Ion Affinity Chromatography (IMAC), developed by Porath et al (1), is based on the interaction of certain protein residues (histidines, cysteines, and to some extent tryptophans) with cations of transition metals. The Copper Chelating Resin is specifically designed for the…

MabSelect SuRe is a protein A affinity medium for capturing MAbs at process scale. MabSelect SuRe has an alkali-tolerant rProtein A ligand that allows the use of rigorous and cost-effective CIP and sanitization protocols based on 0.1 to 0.5 M NaOH. Further, the ligand is protease stable leading to…

Jacalin, or Artocarpus integrifolia lectin, is a tetrameric two-chain lectin with a molecular weight of 66kDa.Jacalin is a α-D-galactose binding lectin purified from jack-fruit ( Artocarpus integrifolia ) seeds. Features Preparing Human IgA free of contaminating IgG …

Con A Sepharose 4B with immobilized Concanavalin A is an affinity chromatography media for capture of glycosylated biomolecules including glycoproteins and glycolipids. Stronger affinity for ?-D-glucose than ?-D-mannose Concanavalin A (Con A) binds molecules that contain ?-D-mannose,…

NHS-Activated Agarose consists of 4% cross-linked agarose that has been activated by the addition of a reactive NHS (N-hydroxysuccinimide) group. The NHS group forms covalent, chemically stable amide bonds with ligand s that contain primary amines. The NHS-Activated Agarose also contains a spacer…

Immobilized metal affinity chromatography (IMAC) resin utilizing nickel (Ni 2+ ) for the purification of 6x histidine tagged proteins. This resin binds to six histidine residues (6x His), a common tag used in protein purification. The resin consists of iminodiacetate coupled to 6% cross-linked…

NHS-activated Sepharose 4 Fast Flow is a pre-activated agarose matrix that increases the choice of coupling chemistries available. Suitable for coupling amino-containing smaller proteins and peptides. NHS pre-activated medium for coupling of small amino-containing proteins and peptides in…

Sepharose CL-2B is a well-proven cross-linked agarose gel filtration base matrix and is frequently used for coupling affinity ligands to the matrix. The matrix is not pre-activated and the user performs all steps in coupling. 2% cross-linked agarose gel filtration media. Proven base matrix for…

Glutathione Resin is designed for the affinity purification of proteins with a glutathione S-transferase (GST) tag. The resin consists of reduced glutathione (GSH) covalently coupled to 6% cross-linked agarose, via a 10-carbon spacer arm. The resin has a binding capacity of >40mg GST/ml resin.…

Calmodulin Resin for the affinity purification of calmodulin binding proteins (CBP), including recombinant proteins with a CBP tag and calmodulin-regulated proteins in eukaryotic cells. The resin is 4% agarose coupled to calmodulin and has a ligand density of approximately 1mg calmodulin/ml resin. …

Ficin (or Ficain) is a cysteine protease enzyme (EC 3.4.22.3) isolated from fig latex is that has the endopeptidase activity to cleave immunoglobulin G molecules in the hinge reason.Ficin is typically used to cleave mouse IgG₁ as this are difficult to cleave with papain and pepsin .In…

ρ-Aminobenzamidine Agarose primary application is for the removal and /or purification of trypsin-like proteases.ρ-aminobenzamidine (PAB) is a synthetic inhibitor of trypsin-like proteases and has been covalently coupled to 6% crosslinked agarose. ρ-aminobenzamidine agaroses have…

Immunoprepitation Kit: Immunoprecipitation (IP) allows purification of a protein from a complex mixture of proteinsusing a specific antibody and a matrix that binds to the antibody. The matrices commonly used are agarose or sepharose bound Protein A or protein A/G PLUS. IP followed by…

Streptavidin Sepharose High Performance is an affinity chromatography medium designed for fast and reliable, high resolution purification of biotinylated biomolecules. Streptavidin immobilized to Sepharose High Performance for purification of biotinylated compounds Extremely useful for…

An important tool in research is the generation of peptide and protein affinity columns for the purification of antibodies and for the discovery of important interacting proteins and cofactors. G-Biosciences offersa complete kit for the coupling of peptides and proteins to agarose through their…

G-Biosciences Immobilized Amino Acids are prepared by coupling amino acid to preactivated 6% cross-linked agarose. The covalent coupling to agarose (resin) is achieved by cross-linking amino group of the amino acid to the preactivated resin. Features: Amino acid coupled to 6%…

Immobilized protein G is for binding the constant domains of immunoglobulin (Ig) molecules (see Figure 1). It is ideal for the easy one-step purification of classes, subclasses and fragments of immunoglobulins for biological fluids and cell culture media. Protein G is a modified form of…

Immobilized Protein A/G consists of recombinant protein A/G ligand covalently immobilized onto 6% highly cross-linked agarose.The dynamic binding capacity will vary depending on several factors such as target antibody, flow rate etc. Protein A/G binds well to IgG subclasses but does not bind…