Affinity Other Media

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  • The Carbohydrate Coupling Resin is designed for the simple and efficient coupling of glycoproteins to a solid agarose support through oxidized sugar groups. The coupling is through the hydrazide group that is immobilized to agarose beads. The resin is ideal for immobilized polyclonal antibodies as…

  • Epoxy-Activated Agarose is preactivated resin used for affinity chromatography. Epoxy-Activated Agarose comprise of high density epoxy groups that form covalent bonds with ligand s containing amine groups, thiol or hydroxyl groups. Epoxide-Acivated Agarose is prepared by immobilization of…

  • Sepharose 6B

    Cytiva

    Sepharose 6B is a well-proven agarose gel filtration base matrix and is frequently used for coupling affinity ligands to the matrix. The matrix is not pre-activated and the user performs all steps in coupling. 6% Agarose gel filtration media. Proven base matrix for coupling affinity ligands 6%…

  • G-Biosciences CDI Amine Reactive Agarose consists of 6% cross-linked agarose activated with CDI (1,1’-carbonyl diimidazole) to form reactive imidazole carbamates. The activation of the resin occurs in solvent and to maintain its activity the resin is supplied in acetone to prevent…

  • Blue Sepharose 6 Fast Flow is Cibacron Blue 3G coupled to Sepharose 6 Fast Flow. This medium is well established as adsorbent for albumin and interferon at both laboratory and process scale Designed for large-scale purification of interferon and albumin. Also used for isolating groups of enzymes…

  • Zinc Chelating Resin

    G-Biosciences

    Immobilized Metal Ion Affinity Chromatography (IMAC), developed by Porath (1), is based on the interaction of certain protein residues (histidines, cysteines, and to some extent tryptophans) with cations of transition metals. The Zinc Chelating Resin is specifically designed for the purification of…

  • Streptavidin Sepharose High Performance is an affinity chromatography medium designed for fast and reliable, high resolution purification of biotinylated biomolecules. Streptavidin immobilized to Sepharose High Performance for purification of biotinylated compounds Extremely useful for…

  • Benzamidine Sepharose 4 Fast Flow (High Sub) is an excellent tool for removal of proteolytic activity from a protein Benzamidine Sepharose 4 Fast Flow (high sub) is based on highly cross-linked 4% agarose enabling high flow rates without losing binding capacity p-aminobenzamidine (pABA)…

  • Calmodulin Sepharose 4B affinity media for purification of proteins with affinity for calmodulin, a highly conserved regulatory protein involved in many cellular processes in eukaryotic cells. For single-step purification of native calmodulin-binding proteins Suitable for tandem affinity…

  • Ni-NTA Resin

    G-Biosciences

    Immobilized Metal Ion Affinity Chromatography (IMAC), developed by Porath (1975), is based on the interaction of certain protein residues (histidines, cysteines, and to some extent tryptophans) with cations of transition metals. The Ni-NTA Resin is specifically designed for the purification of…

  • Amine Magnetic Beads

    G-Biosciences

    G-Biosciences’ Amine Magnetic Beads are 1µm, uniformmagnetic beads with an amine (-NH2) surface functional group. The magnetic beads consist of a single-crystal Fe3O4 sphere core and dextran coating layer. Through chemical modification of dextran, the…

  • Glutathione Resin

    G-Biosciences

    Glutathione Resin is designed for the affinity purification of proteins with a glutathione S-transferase (GST) tag. The resin consists of reduced glutathione (GSH) covalently coupled to 6% cross-linked agarose, via a 10-carbon spacer arm. The resin has a binding capacity of >40mg GST/ml resin.…

  • Immobilized Heparin

    G-Biosciences

    G-Biosciences Immobilized Heparin is a ready-to-use purification resin for a wide range of proteins.The resin consists of 6% cross-linked agarose covalently coupled to heparin through amide bonds.The coupling chemistry used generates a highly stable purification resin that is stable most commonly…

  • lgG Sepharose 6 Fast Flow, from Cytiva , is based on the rigid Sepharose 6 Fast Flow matrix, with human IgG covalently coupled to it. Medium allows high flow rates to be used for rapid and convenient single step purification of protein IgG coupled to the highly cross-linked 6% agarose matrix…

  • Boronate Resin

    G-Biosciences

    Our Boronate resin is designed for the isolation ofribonucleotide and oligonucleotide RNA. The resin consists of boronic acid covalently linked to a polyacrylamide support that offers simple isolation of small molecular weight compounds that have cis-diol groups. Mechanism : The boronic…

  • Concanavalin A (Con A) Agarose consists of Con A coupled to 4% agarose by the cyanogen bromide method. Con A is a tetrameric metalloprotein lectin isolated from Canavalia ensiformis (jack bean). Con A is used for the purification of glycoproteins, polysaccharides and glycolipids as it binds…

  • Lentil Lectin Sepharose media is used for purification of a number of glycoproteins and other carbohydrate containing molecules. The lentil lectin ligand has more equal affinity for ?-D-mannose and ?-D-glucose compared to Con A. Binds molecules that contain ?-D-mannose, ?-D-glucose, and…

  • Immobilized Protein A/G consists of recombinant protein A/G ligand covalently immobilized onto 6% highly cross-linked agarose.The dynamic binding capacity will vary depending on several factors such as target antibody, flow rate etc. Protein A/G binds well to IgG subclasses but does not bind…

  • Sepharose CL-6B is a well-proven cross-linked agarose gel filtration base matrix and is frequently used for coupling affinity ligands to the matrix. The matrix is not pre-activated and the user performs all steps in coupling. 6% cross-linked agarose gel filtration media. Proven base matrix for…

  • Immobilized Reductant

    G-Biosciences

    Reducing agents are used in the reduction of disulfide bonds of proteins and peptides. It is often necessary to remove the reducing agents from the protein/peptide solutions to prevent them from interfering with subsequent procedures. However, for small proteins, and particularly peptides, it is…

  • Thiophilic Resin

    G-Biosciences

    Thiophilic adsorption or thiophilic chromatography is a routinely used technique for the low cost, simple purification of immunoglobulins. Thiophilic adsorption was first developed by Porath et al 1 in 1984 and is a group specific, salt-dependent purification technique that has distinct affinity…

  • Epoxy-activated Sepharose 6B is a pre-activated medium for immobilization of various ligands including sugars through coupling of hydroxy, amino or thiol groups on the ligand to Sepharose 6B via a 12-atom hydrophilic spacer arm Epoxy-activated Sepharose 6B can be used to couple sugars and other…

  • Immobilized Procainamide

    G-Biosciences

    Immobilized Procainamide is designed for affinity purification of butyrylcholinesterase (BuChE), which is also known as non-specific cholinesterase. Butyrylcholinesterase can be purified by batch adsorption on immobilized Procainamide followed by ion-exchange chromatography on DEAE-Sepharose…

  • Immobilized Jacalin

    G-Biosciences

    Jacalin, or Artocarpus integrifolia lectin, is a tetrameric two-chain lectin with a molecular weight of 66kDa.Jacalin is a α-D-galactose binding lectin purified from jack-fruit ( Artocarpus integrifolia ) seeds. Features Preparing Human IgA free of contaminating IgG …

  • NHS-activated Sepharose 4 Fast Flow is a pre-activated agarose matrix that increases the choice of coupling chemistries available. Suitable for coupling amino-containing smaller proteins and peptides. NHS pre-activated medium for coupling of small amino-containing proteins and peptides in…

  • Ni Sepharose 6 Fast Flow is a BioProcess medium that combines the advantages of Ni?? for purification of histidine tagged proteins, with the well industrially established properties of the Sepharose Fast Flow platform. Designed for scaling up purification of histidine-tagged proteins High…

  • Ni-NTA Magnetic Beads

    G-Biosciences

    G-Biosciences’ Ni-NTA Magnetic Beads are 3µm beads designed for the rapid purification of X His-tagged proteins. Ni-NTA Magnetic Beads have nitrilotriacetic acid (NTA) groups with charged nickel covalently bound to the surface dextran of the beads.Due to the high affinity, Ni-NTA…

  • Immobilized protein G is for binding the constant domains of immunoglobulin (Ig) molecules (see Figure 1). It is ideal for the easy one-step purification of classes, subclasses and fragments of immunoglobulins for biological fluids and cell culture media. Protein G is a modified form of…

  • Immobilized Metal Ion Affinity Chromatography (IMAC), developed by Porath (1975), is based on the interaction of certain protein residues (histidines, cysteines, and to some extent tryptophans) with cations of transition metals.G-Sep™NI NTAAgarose Fast Flow is specifically designed for the…

  • The Sulfhydryl Coupling Resin is designed for the simple and efficient coupling of peptides and proteins to a solid 6% agarose support through free sulfhydryl groups (-SH).The iodoacetyl groups of the Sulfhydryl Coupling Resin specifically react with free sulfhydryls to form covalent, permanent…

  • Sepharose CL-2B is a well-proven cross-linked agarose gel filtration base matrix and is frequently used for coupling affinity ligands to the matrix. The matrix is not pre-activated and the user performs all steps in coupling. 2% cross-linked agarose gel filtration media. Proven base matrix for…

  • NHS Sepharose High Performance Simple, small-scale enrichment of target proteins using pull-down technique. Antibody or capturing molecule covalently immobilized via free NH2 groups. Simple coupling, carried out near neutral pH. Fast and flexible protocol for the capture step, with elution…

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