Affinity Other Media

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Affinity Other Media

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  • Epoxy-Activated Agarose is preactivated resin used for affinity chromatography. Epoxy-Activated Agarose comprise of high density epoxy groups that form covalent bonds with ligand s containing amine groups, thiol or hydroxyl groups. Epoxide-Acivated Agarose is prepared by immobilization of…

  • Immobilized Protein A/G consists of recombinant protein A/G ligand covalently immobilized onto 6% highly cross-linked agarose.The dynamic binding capacity will vary depending on several factors such as target antibody, flow rate etc. Protein A/G binds well to IgG subclasses but does not bind…

  • Immobilized Biotin

    G-Biosciences

    Immobilized Biotin Resin is designed for the high affinity chromatography purifications of avidin, streptavidin and Neutravidin protein. The resin consists of biotin coupled to 6% cross-linked agarose. Biotin, a 244 Dalton vitamin (Vitamin H) molecule, exhibits an extraordinary binding…

  • Ni Sepharose 6 Fast Flow is a BioProcess medium that combines the advantages of Ni?? for purification of histidine tagged proteins, with the well industrially established properties of the Sepharose Fast Flow platform. Designed for scaling up purification of histidine-tagged proteins High…

  • Streptavidin Sepharose High Performance is an affinity chromatography medium designed for fast and reliable, high resolution purification of biotinylated biomolecules. Streptavidin immobilized to Sepharose High Performance for purification of biotinylated compounds Extremely useful for…

  • Blue Sepharose 6 Fast Flow is Cibacron Blue 3G coupled to Sepharose 6 Fast Flow. This medium is well established as adsorbent for albumin and interferon at both laboratory and process scale Designed for large-scale purification of interferon and albumin. Also used for isolating groups of enzymes…

  • Concanavalin A (Con A) Agarose consists of Con A coupled to 4% agarose by the cyanogen bromide method. Con A is a tetrameric metalloprotein lectin isolated from Canavalia ensiformis (jack bean). Con A is used for the purification of glycoproteins, polysaccharides and glycolipids as it binds…

  • Sepharose 6B

    Cytiva

    Sepharose 6B is a well-proven agarose gel filtration base matrix and is frequently used for coupling affinity ligands to the matrix. The matrix is not pre-activated and the user performs all steps in coupling. 6% Agarose gel filtration media. Proven base matrix for coupling affinity ligands 6%…

  • Con A Sepharose 4B with immobilized Concanavalin A is an affinity chromatography media for capture of glycosylated biomolecules including glycoproteins and glycolipids. Stronger affinity for ?-D-glucose than ?-D-mannose Concanavalin A (Con A) binds molecules that contain ?-D-mannose,…

  • Immobilized protein G is for binding the constant domains of immunoglobulin (Ig) molecules (see Figure 1). It is ideal for the easy one-step purification of classes, subclasses and fragments of immunoglobulins for biological fluids and cell culture media. Protein G is a modified form of…

  • Heparin Sepharose 6 Fast Flow is a well established BioProcess affinity resin designed for capture or intermediate purification steps. This resin is extensively used for industrial purification of several marketed plasma proteins. Industrial purification of antithrombin III, coagulation factors…

  • Glutathione Resin

    G-Biosciences

    Glutathione Resin is designed for the affinity purification of proteins with a glutathione S-transferase (GST) tag. The resin consists of reduced glutathione (GSH) covalently coupled to 6% cross-linked agarose, via a 10-carbon spacer arm. The resin has a binding capacity of >40mg GST/ml resin.…

  • Ni-NTA Resin

    G-Biosciences

    Immobilized Metal Ion Affinity Chromatography (IMAC), developed by Porath (1975), is based on the interaction of certain protein residues (histidines, cysteines, and to some extent tryptophans) with cations of transition metals. The Ni-NTA Resin is specifically designed for the purification of…

  • Cobalt Chelating Resin

    G-Biosciences

    Immobilized Metal Ion Affinity Chromatography (IMAC), developed by Porath (1), is based on the interaction of certain protein residues (histidines, cysteines, and to some extent tryptophans) with cations of transition metals. The Cobalt Chelating Resin is specifically designed for the…

  • Immunoprepitation Kit: Immunoprecipitation (IP) allows purification of a protein from a complex mixture of proteinsusing a specific antibody and a matrix that binds to the antibody. The matrices commonly used are agarose or sepharose bound Protein A or protein A/G PLUS. IP followed by…

  • NHS-activated Sepharose 4 Fast Flow is a pre-activated agarose matrix that increases the choice of coupling chemistries available. Suitable for coupling amino-containing smaller proteins and peptides. NHS pre-activated medium for coupling of small amino-containing proteins and peptides in…

  • Lectins are proteins or glycoproteins that have at least one noncatalytic domain that binds specifically and reversibly to monosaccharides or oligosaccharides. Lectins are important as they are found in applications in science, medicine, agriculture, pharmaceutical companies, biotechnology and…

  • Epoxy-activated Sepharose 6B is a pre-activated medium for immobilization of various ligands including sugars through coupling of hydroxy, amino or thiol groups on the ligand to Sepharose 6B via a 12-atom hydrophilic spacer arm Epoxy-activated Sepharose 6B can be used to couple sugars and other…

  • Ni-NTA Magnetic Beads

    G-Biosciences

    G-Biosciences’ Ni-NTA Magnetic Beads are 3µm beads designed for the rapid purification of X His-tagged proteins. Ni-NTA Magnetic Beads have nitrilotriacetic acid (NTA) groups with charged nickel covalently bound to the surface dextran of the beads.Due to the high affinity, Ni-NTA…

  • MabSelect

    Cytiva

    MabSelect is a protein A affinity medium designed for capturing MAbs from large sample volumes. High-flow agarose matrix allows for high flow velocities at production scale enabling the processing of more than 10 000 L of feed in one working day. Oriented coupling of the ligand and optimized…

  • G-Biosciences CDI Amine Reactive Agarose consists of 6% cross-linked agarose activated with CDI (1,1’-carbonyl diimidazole) to form reactive imidazole carbamates. The activation of the resin occurs in solvent and to maintain its activity the resin is supplied in acetone to prevent…

  • Sepharose CL-6B is a well-proven cross-linked agarose gel filtration base matrix and is frequently used for coupling affinity ligands to the matrix. The matrix is not pre-activated and the user performs all steps in coupling. 6% cross-linked agarose gel filtration media. Proven base matrix for…

  • Sepharose CL-4B is a well-proven cross-linked agarose gel filtration base matrix and is frequently used for coupling affinity ligands to the matrix. The matrix is not pre-activated and the user performs all steps in coupling. 4% cross-linked agarose gel filtration media. Proven base matrix for…

  • Sepharose 4B

    Cytiva

    Sepharose 4B is a well-proven agarose gel filtration base matrix and is frequently used for coupling affinity ligands to the matrix. The matrix is not pre-activated and the user performs all steps in coupling. 4% Agarose gel filtration media. Proven base matrix for coupling affinity ligands 4%…

  • Nickel Chelating Resin

    G-Biosciences

    Immobilized metal affinity chromatography (IMAC) resin utilizing nickel (Ni 2+ ) for the purification of 6x histidine tagged proteins. This resin binds to six histidine residues (6x His), a common tag used in protein purification. The resin consists of iminodiacetate coupled to 6% cross-linked…

  • Immobilized Metal Ion Affinity Chromatography (IMAC), developed by Porath (1975), is based on the interaction of certain protein residues (histidines, cysteines, and to some extent tryptophans) with cations of transition metals.G-Sep™NI NTAAgarose Fast Flow is specifically designed for the…

  • CNBr-activated Sepharose 4 Fast Flow is a well established, pre-activated chromatography medium for coupling of large amino-containing ligands. CNBr-activated BioProcess medium designed for coupling of large amino-containing ligands. Easy, rapid and efficient coupling This resin allows for…

  • Boronate Resin

    G-Biosciences

    Our Boronate resin is designed for the isolation ofribonucleotide and oligonucleotide RNA. The resin consists of boronic acid covalently linked to a polyacrylamide support that offers simple isolation of small molecular weight compounds that have cis-diol groups. Mechanism : The boronic…

  • Benzamidine Sepharose 4 Fast Flow (High Sub) is an excellent tool for removal of proteolytic activity from a protein Benzamidine Sepharose 4 Fast Flow (high sub) is based on highly cross-linked 4% agarose enabling high flow rates without losing binding capacity p-aminobenzamidine (pABA)…

  • Zinc Chelating Resin

    G-Biosciences

    Immobilized Metal Ion Affinity Chromatography (IMAC), developed by Porath (1), is based on the interaction of certain protein residues (histidines, cysteines, and to some extent tryptophans) with cations of transition metals. The Zinc Chelating Resin is specifically designed for the purification of…

  • Immobilized Reductant

    G-Biosciences

    Reducing agents are used in the reduction of disulfide bonds of proteins and peptides. It is often necessary to remove the reducing agents from the protein/peptide solutions to prevent them from interfering with subsequent procedures. However, for small proteins, and particularly peptides, it is…

  • Amine Magnetic Beads

    G-Biosciences

    G-Biosciences’ Amine Magnetic Beads are 1µm, uniformmagnetic beads with an amine (-NH2) surface functional group. The magnetic beads consist of a single-crystal Fe3O4 sphere core and dextran coating layer. Through chemical modification of dextran, the…

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