Buffer Solutions

A buffer is a solution created to resist pH changes when introducing internal and external influences. Buffers also maintain intercellular and extracellular pH within the physiological range, providing minimal interference in biological processes. At Thomas Scientific, we have an extensive range of buffers available, including sodium phosphate buffers, blue buffers and LDS sample buffers. We offer a selection of buffers and native gels that carry both positive and negative charges. Buffers are suitable for biochemical research applications such as cell cultivation, isolation of cells, and enzyme assays.

Buffer Solutions

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  • Color-Coded pH Buffer Solutions

    chemForce® (powered by Thomas Scientific)

    Harmonized #: 3822.00.0002|5090 Shelf Life (months): 24 Specific Gravity: 1.00 DOT: Not regulated Physical State: Liquid Flash Point: Not applicable Solubility: Miscible Storage Temperature: Ambient Storage Code: General Traceable to NIST standards

  • Color-Coded pH Buffers

    Standardized and certified traceable to NIST Standard Color-coded for quick reference Accuracy ±0.01 pH at 25°C pH temperature chart, lot numbers and expiration dates on each label pH 4.0 buffer is colored red, 7.0 buffer is yellow or green and 10.0 buffer is blue. Thomas numbers 4120R54,...

  • Phosphate buffered saline pH 7.4, contains TWEEN® 20, dry powder

    Synonyms: PBS MDL Number: MFCD00131855 Reconstitution: Contents of one pouch, when dissolved in one liter of distilled or deionized water, will yield 0.01 M phosphate buffered saline (NaCl 0.138 M; KCl - 0.0027 M); TWEEN ® 20 - 0.05%, pH 7.4, at 25-á°C. Legal Information: TWEEN is a...

  • Phosphate Buffered Saline (PBS)

    Phosphate Buffered Saline (pH of 7.5) is recommended as a diluent for quantitative culturing or as a rinsing agent in microbiological, immunological, histological and molecular testing procedures.

  • SIGMA Phosphate buffered saline, tablet

    Synonyms: PBS Application: Phosphate Buffered Saline tablets have been specifically developed for use in immunoassay procedures. Reconstitution: One tablet dissolved in 200 mL of deionized water yields 0.01 M phosphate buffer, 0.0027 M potassium chloride and 0.137 M sodium chloride, pH 7.4, at...

  • 1X PBS, pH 7.4

    Quality Biological

    PBS (phosphate buffered saline) is a balanced salt solution used for a variety biological and cell culture applications, such as washing cells before dissociation, transporting cells or tissue, diluting cells for counting, and preparing reagents. Endotoxin and Mycoplasma tested Applications PBS is...

  • Buffer, Reference Standard, pH 12.45 ± 0.01 at 25°C

    pH made Easy. Everything you need and want in a pH Buffer. NIST Traceability. Consistent, reliable performance. The tightest specification (+/- 0.01 pH unit) on all buffers. Long Expiration dates. Multiple sizes to fit your usage and budget constraints. All combined in one of the most comprehensive...

  • TG-SDS Buffer (Tris-Glycine-SDS) 10X Solution

    The Bio Basic Inc. TG-SDS buffer (tris-glycine-SDS) 10x solution is suitable for laboratory and research use.

  • Acetate Buffer Solution, PH 3.5, For Usp Heavy Metals Testing

    120ML Acetate Buffer Solution, ph 3.5, for USP heavy metals testing UN2790

  • Colored pH Buffer Standards

    Inorganic Ventures

    Inorganic Ventures' pH Standard is a certified reference material set in a water matrix for stability. This standard is Traceable to NIST and manufactured in accordance with our stringent Quality Assurance guidelines. One year Expiration Date.

  • Ricca Acetate Buffer

    Ricca Chemical

    The Ricca Chemicals 500 mL acetate buffer is mainly used in research facilities and chemical laboratories to conduct wide range of experiments. It is available in sturdy bottle that endures heavy and rigorous usage. This buffer is used for cyanide determination. Application: For Cyanide...

  • Orion® pH Buffers

    Thermo Scientific Orion

    Orion pH buffers are manufactured under ISO 9000 quality standards. Buffers are color-coded for ease of use, packaged with buffer/temperature tables for accurate calibrations and NIST traceability. All-in-One Buffer kit (1233M21) includes pint bottles of 4 (pink), 7 (yellow) and 10 (blue) buffers,...

  • VeriSpec® pH Buffer and Conductivity Standard

    Ricca’s VeriSpec® pH Buffer and Conductivity Standard Certified Reference Materials are ISO 17025/17034 accredited to provide the highest level of quality assurance. These products have undergone rigorous evaluations of manufacturing methods, quality control, stability, and homogeneity. As...

  • Traceable® pH Buffer Standards (CRM)

    The most accurate pH Standards available anywhere Certified Reference Material Traceable® Certificate supplied Highest accuracy available Traceable® pH Standards, Certified Reference Materials, are 100% compatible with all instruments and probes. Accuracy for the Traceable® Buffers at...

  • Technical pH Buffer Solutions

    Technical buffering solutions for pH 4.01, pH 7.00 and pH 10.01: pH 4.01, pH 7.00 and pH 10.01 are available in the practical 50mL bottles as well as in 1L special bottles with dosing device and pour spout.  The buffer 10.0 is a special case: It is only still suitable for inoLab®...

  • Traceable® ph Buffer Standards (CRM), Color Coded

    labForce® (powered by Thomas Scientific)

    The MOST accurate pH Standards available anywhere now color-coded for convenience Traceable® pH Standards, Certified Reference Materials, are 100% compatible with all instruments and probes. Accuracy for the Traceable® Buffer at 25°C is ±0.010 pH—the most precise available....

  • Tris Base, Ultra Pure

    RPI (Research Products International)

    Highly purified for use in molecular biology applications. Moisture:<0.2 % color: <20 apha (colorless) heavy metals (as pb): <0.0001% insoluble matter: <0.005% heavy metals: 5 ppm pka (20°c): 8.1 ± 0.1<>

  • EDTA, Disodium Salt

    IBI Scientific

    EDTA is commonly used in biological and electrophoresis buffer systems. Store at room temperature. Protect from moisture. Irritant. Causes irritation to eyes, skin and mucous membranes. Avoid breathing dust. Do not take internally. Wash thoroughly after handling. See Material Safety Data Sheet for...

  • TISAB II, Standardized

    Composition: Water 84.82%, Acetic Acid 5.98%, Sodium Chloride 5.80%, Sodium Hydroxide 3%, 1,2-Cyclohexylenedimaine Tetraacetic Acid (CDTA) 0.40% Boiling Point: Approximately 100°C Density: 1.03 Melting Point: Approximately 0°C Color: Colorless liquid Physical State: Liquid pH Range: 5.0 to...

  • Clear pH Buffer Solutions

    chemForce® (powered by Thomas Scientific)

    Harmonized #: 2806.10.0000|0000 Shelf Life (months): 24 Specific Gravity: 1.00 DOT: Not regulated Appearance: Colorless Physical State: Liquid Flash Point: Not applicable Solubility: Miscible Storage Temperature: Ambient Storage Code: General

  • Color-Coded pH Buffer Solution Set

    chemForce® (powered by Thomas Scientific)

    Size: 3 x 500 mL Harmonized #: 3822.00.0002|5090 Shelf Life (months): 24 Specific Gravity: 1.00 DOT: Not regulated Appearance: Color coded Physical State: Liquid pH: 4, 7, 10 Flash Point: Not applicable Solubility: Miscible Storage Temperature: Ambient Storage Code: General

  • Traceable® ColorCode pH One-Shot™ Assorted

    labForce® (powered by Thomas Scientific)

    The MOST accurate pH Standards available anywhere now color-coded for convenience Traceable® pH Standards, Certified Reference Materials, are 100% compatible with all instruments and probes. Accuracy for the Traceable® Buffer at 25°C is ±0.010 pH—the most precise available....

  • High-Accuracy pH Buffer Solutions

    pH Calibration buffers for tight tolerances For meters with 0.001 resolution Buffers are color-coded for easy visual identification.

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What is nuclease free water?

Nuclease-free water is highly purified water removed of nucleases. This type of water is essential in molecular biology experiments, such as PCR, RNA sequencing, or DNA extraction, where the integrity of nucleic acids is crucial. By using nuclease-free water, scientists ensure that their samples won't be degraded by unwanted enzymes, preserving the accuracy and reliability of their experiments.

Why is nuclease free water used in PCR?

Nuclease-free water is essential in PCR (Polymerase Chain Reaction) to prevent degradation of the DNA template and primers. Nucleases are enzymes that can degrade DNA or RNA, which would compromise the accuracy and success of the PCR process.