Buffer Solutions

A buffer is a solution created to resist pH changes when introducing internal and external influences. Buffers also maintain intercellular and extracellular pH within the physiological range, providing minimal interference in biological processes. At Thomas Scientific, we have an extensive range of buffers available, including sodium phosphate buffers, blue buffers and LDS sample buffers. We offer a selection of buffers and native gels that carry both positive and negative charges. Buffers are suitable for biochemical research applications such as cell cultivation, isolation of cells, and enzyme assays.

Buffer Solutions

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  • Clear pH Buffer Solutions

    chemForce® (powered by Thomas Scientific)

    Harmonized #: 2806.10.0000|0000 Shelf Life (months): 24 Specific Gravity: 1.00 DOT: Not regulated Appearance: Colorless Physical State: Liquid Flash Point: Not applicable Solubility: Miscible Storage Temperature: Ambient Storage Code: General

  • TBST [10X]; Tris buffered saline with Tween-20

     A 10X Concentrated solution of Tris Buffered Saline with Tween® 20.    The 10X Concentration is 100mM Tris.HCl, 150mM NaCl, 0.5% Tween® 20 at pH7.5.   The 1X Concentration is 10mM Tris.HCl, 15mM NaCl, 0.05% Tween® 20 at pH7.5.   TBST is commonly used...

  • Color-Coded pH Buffers

    Standardized and certified traceable to NIST Standard Color-coded for quick reference Accuracy ±0.01 pH at 25°C pH temperature chart, lot numbers and expiration dates on each label pH 4.0 buffer is colored red, 7.0 buffer is yellow or green and 10.0 buffer is blue. Thomas numbers 4120R54,...

  • Orion® pH Buffers

    Thermo Scientific Orion

    Orion pH buffers are manufactured under ISO 9000 quality standards. Buffers are color-coded for ease of use, packaged with buffer/temperature tables for accurate calibrations and NIST traceability. All-in-One Buffer kit (1233M21) includes pint bottles of 4 (pink), 7 (yellow) and 10 (blue) buffers,...

  • Buffer Standard, pH 7.00

    Meets requirements of both ISO 17025 and ISO 17034 Certified Reference Materials, made under the strictest standards for stability, homogeneity, and accuracy Tested to tolerances of 0.01 pH units Supported by detailed Certificate of Analysis including temperature variation values and stated...

  • Buffer Calibration Solutions

    Any pH measurement is only as accurate as the buffer solution used for calibration. These buffer solutions are traceable to primary standards set by NIST. Buffers 5001A40 through 5001A45 come with a quality assurance certificate per DKD and are particularly useful in quality assurance systems....

  • pH Electrode Solutions

    Thermo Scientific Orion

    Electrode Storage Solution 475 mL plastic bottle pH of solution is 6.2 to 7.4 For short term storage of all electrodes for one day to a week. Electrode Filling Solution Internal filling solution for Ross-type electrodes (3M KCl). In packages of five 60 mL bottles.

  • Traceable® pH Buffer Standards (CRM)

    The most accurate pH Standards available anywhere Certified Reference Material Traceable® Certificate supplied Highest accuracy available Traceable® pH Standards, Certified Reference Materials, are 100% compatible with all instruments and probes. Accuracy for the Traceable® Buffers at...

  • Potassium Phosphate, Monobasic, ACS Grade

    RPI (Research Products International)

    Potassium Phosphate, Monobasic, ACS Grade

  • 1X TE Buffer

    IBI Scientific

    Sterile Endotoxin Free pH = 8.3 IBI TE buffer is packaged in medical grade non-leaching polycarbonate bottles to ensure purity levels and sterility are maintained. This 1X TE Buffer is certified nuclease free and endotoxin free by way of LAL testing to < 0.05 EU/ml. This 1X TE buffer has a pH of...

  • pH Buffer Solutions

    Choose from economical solution bottles or individually sealed pH buffer pouches. NIST traceable materials Labeled with pH vs. temperature tables for accurate calibration All Oakton pH buffer solutions are freshness dated, and standardized against NIST-traceable references to ensure quality....

  • Buffer Solution, pH 12.00

    pH (@ 25°C) 12.00 +/-0.08 NIST Traceable

  • Buffer Standard, pH 10.00

    Meets requirements of both ISO 17025 and ISO 17034 Certified Reference Materials, made under the strictest standards for stability, homogeneity, and accuracy Tested to tolerances of 0.01 pH units Supported by detailed Certificate of Analysis including temperature variation values and stated...

  • Buffer Standard, pH 12.00

    Meets requirements of both ISO 17025 and ISO 17034 Certified Reference Materials, made under the strictest standards for stability, homogeneity, and accuracy Tested to tolerances of 0.01 pH units Supported by detailed Certificate of Analysis including temperature variation values and stated...

  • Potato Dextrose Broth, Sterile

    Potato Dextrose Broth

  • 1M TRIS-HCl, pH 7.5

    Quality Biological

    Tris is compatible with many enzymes in molecular biology, for example DNA modifying enzymes, because its buffering range is between 7.1-8.9. The lower pH 7.4 can be associated more with maintaining RNA, however, the pH 8.0 works equally as well with both DNA and RNA nucleic acids. Applications Tris...

  • Color-Coded pH Buffer Solutions

    chemForce® (powered by Thomas Scientific)

    Harmonized #: 3822.00.0002|5090 Shelf Life (months): 24 Specific Gravity: 1.00 DOT: Not regulated Physical State: Liquid Flash Point: Not applicable Solubility: Miscible Storage Temperature: Ambient Storage Code: General Traceable to NIST standards

  • Traceable® ph Buffer Standards (CRM), Color Coded

    labForce® (powered by Thomas Scientific)

    The MOST accurate pH Standards available anywhere now color-coded for convenience Traceable® pH Standards, Certified Reference Materials, are 100% compatible with all instruments and probes. Accuracy for the Traceable® Buffer at 25°C is ±0.010 pH—the most precise available....

  • Buffer, Phosphate, pH 7.2

    For Biochemical Oxygen Demand (BOD) testing Formula: Mixture

  • Color-Coded pH Buffer Solution Set

    chemForce® (powered by Thomas Scientific)

    Size: 3 x 500 mL Harmonized #: 3822.00.0002|5090 Shelf Life (months): 24 Specific Gravity: 1.00 DOT: Not regulated Appearance: Color coded Physical State: Liquid pH: 4, 7, 10 Flash Point: Not applicable Solubility: Miscible Storage Temperature: Ambient Storage Code: General

  • Traceable® ColorCode pH One-Shot™ Assorted

    labForce® (powered by Thomas Scientific)

    The MOST accurate pH Standards available anywhere now color-coded for convenience Traceable® pH Standards, Certified Reference Materials, are 100% compatible with all instruments and probes. Accuracy for the Traceable® Buffer at 25°C is ±0.010 pH—the most precise available....

  • High-Accuracy pH Buffer Solutions

    pH Calibration buffers for tight tolerances For meters with 0.001 resolution Buffers are color-coded for easy visual identification.

  • Certipur® Buffer Solution, pH 7.00

    pH: 7.0 (H2O, 20°C) Solubility: (20°C) soluble Melting Point: -5°C Boiling Point: 109°C Density: 1.01 g/cm3 (20°C)

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What is nuclease free water?

Nuclease-free water is highly purified water removed of nucleases. This type of water is essential in molecular biology experiments, such as PCR, RNA sequencing, or DNA extraction, where the integrity of nucleic acids is crucial. By using nuclease-free water, scientists ensure that their samples won't be degraded by unwanted enzymes, preserving the accuracy and reliability of their experiments.

Why is nuclease free water used in PCR?

Nuclease-free water is essential in PCR (Polymerase Chain Reaction) to prevent degradation of the DNA template and primers. Nucleases are enzymes that can degrade DNA or RNA, which would compromise the accuracy and success of the PCR process.