Buffers

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  • Gram-negative Lysis Buffer is an extraction buffer for soluble proteins from Gram-negative bacteria. It is a proprietary improvement on the lysozyme based lysis in combination with various salts and agents, which allows extraction of soluble proteins. Depending on the application, additional agents…

  • Trypsin Digestion Buffer

    G-Biosciences

    The Trypsin Digestion Mix provides optimal buffered conditions for in gel trypsin digestion of proteins. Suitable for 100 protein spots.

  • TBS, Tris buffered saline [10X] 100mM Tris HCl, 1.5M NaCl, pH 7.5

  • Tris-Glycine [10X]

    G-Biosciences

    Features 0.25M Tris, 1.92M Glycine, pH 8.3

  • SSC [20X]

    G-Biosciences

    Saline-Sodium Citrate HybridizationBuffer [20X] controls stringency during the washing steps in Southern blotting, insitu hybridization, DNA microarray or Northern blotting. 20X SSC may be used to prevent the drying of agarose gels during a vacuum transfer. Features 0.3M trisodium…

  • STM Buffer

    G-Biosciences

    For growing cells and for competent cells preparation Features 270mM Sucrose, 10mM Tris-HCl, ph 7.5, 1mM MgCl2

  • Tris HCl

    G-Biosciences

      Features       Synonym: Tris(hydroxymethyl)aminomethanehydrochloride CAS#: 1185-53-1 Molecular Formula: C4H11NO3 · HCl Molecular Weight: 157.6    

  • A selection of 12 x 25ml molecular biology relevant buffers. These buffers are routinely used in molecular biology and are all DNase and RNase free. The buffers supplied are listed below. Features Molecular Biology Universal Kit Ammonium acetate [5M] Calcium chloride [1M] …

  • SM Buffer

    G-Biosciences

    Features 50mM Tris.HCl (pH7.5), 100mM NaCl, 8mM MgSO4, 0.01% Gelatin

  • Based on Efficient ™ Western Transfer Buffer, the High Molecular Weight Transfer buffer is designed to facilitate the transfer of notoriously difficult high molecular weight proteins (>70kDa) during Western blotting. Supplied as 1 liter of a 5X concentrated solution.

  • Tricine

    G-Biosciences

    Features Synonym: N-[Tris(hydroxymethyl)methyl]glycine, 3-[(3-Cholamidopropyl)dimethylammonio]propanesulfonic acid Linear Formula: (HOCH2)3CNHCH2CO2H Molecular Weight: 179.17 CAS Number: 5704-04-1   Applications …

  • STET Solution

    G-Biosciences

    Buffer for DNA/RNA extraction and purification. Features 8% w/v sucrose, 5% v/v Triton® X-100, 50mM EDTA, 50mM Tris-HCl, pH 8.0

  • Sodium Acetate

    G-Biosciences

    Features Synonym: Acetic acid sodium salt CAS#: 127-09-3 Molecular Formula: C2H3O2Na Molecular Weight: 82.03

  • Features 8M Guanidine.HCl, pH 1.5 Streptavidin resin also available

  • Phosphate Buffers

    G-Biosciences

    Phosphate buffers are buffer solutions commonly used in biological research. They are water based salt solution containing sodium phosphate monobasic and dibasic at a fixed pH. Competitive remarks and statements Feature statements Application statements Features Useful pH…

  • Features 2M Tris-Acetate, 0.05M EDTA (pH8.3)    

  • SDS-PAGE Running Buffer

    G-Biosciences

    Features SDS-PAGE Running Buffer (10X) Compatible with Tris-Glycine gels Separate proteins in the range 6-200 kDa under denaturing conditions 1 X concentration: 25mM Tris Base, 192 mM Glycine, 0.1% SDS Applications Tris-Glycine/SDS Running Buffer [10X] is compatible…

  • GST Elution Buffer

    G-Biosciences

    Features Supplied with dry glutathione to limit oxidation Glutathione resin also available

  • Features Synonym:  1,4-Piperazinediethanesulfonic acid sesquisodium salt, Piperazine-1,4-bis(2-ethanesulfonic acid) sesquisodium salt CAS#:  100037-69-2 Molecular Formula: …

  • Saline-Sodium Citrate Hybridization Buffer [20X] controls stringency during the washing steps in Southern blotting, in situ hybridization, DNA microarray or Northern blotting. 20X SSC may be used to prevent the drying of agarose gels during a vacuum transfer. Features …

  • Tris

    G-Biosciences

    Tris is an abbreviation of the organic compound known as tris(hydroxymethyl)aminomethane, with the formula (HOCH2)3CNH2. Tris is extensively used in as a major component in buffer solutions. This 1M solution is has a pH of 9.0. Other 1M Tris solutions are…

  • Features 20mM NaPO4, 0.15M NaCl (pH 7.5) Streptavidin resin also available

  • femto TBST™ and femto PBST™ will enhance sensitivity of your immunoassays by minimizing the "washing out" of immuno agents or immuno complexes, a common problem associated with classical TBST and PBST buffers. The wash buffers aid in the generation of cleaner backgrounds,…

  • A 10X Concentrated solution of Phosphate Buffered Saline with Tween® 20. The 10X Concentration is 80mM Na2HPO4, 1.5M NaCl, 20mM KH2PO4, 30mM KCl, 0.5% Tween® 20, pH 7.4.   PBST is commonly used as a wash solution for Western blot…

  • The SDS-PAGE Sample Loading Buffers are suitable for loading protein samples on to the SDS-polyacrylamide gels. The buffers are provided in 2X and 6X concentrations containing Tris-HCl, glycerol, SDS and bromophenol blue (BPB) in recommended concentrations and is stable at room temperature. …

  • JAW™ MES Buffered Saline Packs are ready to use dry-blend powder pouches designed to make MES buffer saline which is amine and carboxyl-free and used for carbodiimide (EDC) crosslinking and conjugation reactions. Features: Each Pack is dissolved in 500 ml deionized water to get…

  • Tris-Glycine powder pouches are dry-blend, pre-measured powder packs used to make standard transfer buffer or running buffer for native PAGE. Features : Dissolve contents of packet in deionized water and 100 methanol to make up to final volume of 500 ml. The resulting buffer makes 25mM…

  • Chaotropic extraction buffers that preserve the native charge of proteins making them s uitable for sample extraction for 2D gels One of the most important considerations before running 2D gel electrophoresis is the choice of protein solubilization buffers. A suitable buffer must…

  • Tris, pH7.0 [1M]

    G-Biosciences

    Tris is an abbreviation of the organic compound known as tris(hydroxymethyl)aminomethane, with the formula (HOCH2)3CNH2. Tris is extensively used in as a major component in buffer solutions. This 1M solution is has a pH of 7.0.

  • Tris-EDTA-NaCl Buffer solution at pH 8.0. A customer enquired about this buffer to make as custom and we also added as a new product. Features Precision in concentration and pH Guaranteed reproducibility Applications As a buffering mix

  • Zymogram Sample Buffer

    G-Biosciences

    Zymography is an electrophoretic technique, based on SDS-PAGE, that includes an enzyme substrate copolymerized with the polyacrylamide gel. Samples are prepared in Zymogram Sample Buffer without boiling to preserve the structure and activity of the enzyme. Following electrophoresis, the SDS is…

  • Tris-Acetate/SDS running buffer is used to resolve high molecular weight proteins (36-400 kDa) under denaturing conditions on Tris/Acetate gels Features Tris-Acetate/SDS running buffer is compatible with Tris/Acetate gels Separate high molecular weight proteins (36-400 kDa) under…

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