G-Biosciences Buffer Solutions
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Denhardt Solution [100X]
G-BiosciencesSuitable for nucleic acid hybridization. Denhardt's solution is a mixture of high-molecular weight polymers capable of saturating non-specific binding sites and artificially increasing the concentration of available probe. Features 2% Ficoll 400 0.3M sodium chloride 2% PVP…
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Tris-Acetate/SDS Running Buffer [20X]
G-BiosciencesTris-Acetate/SDS running buffer is used to resolve high molecular weight proteins (36-400 kDa) under denaturing conditions on Tris/Acetate gels Features Tris-Acetate/SDS running buffer is compatible with Tris/Acetate gels Separate high molecular weight proteins (36-400 kDa) under…
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TE Buffer [10X]
G-BiosciencesSynonyms: TE Buffer, Tris-EDTA Buffer, T10E1 Buffer The TE Buffer is a common molecular biology buffer used for protection of DNA and RNA from degradation. The Tris buffering agent and EDTA metal chelating properties help protect DNA and RNA. The TE Buffer [10X] is offered in 0.5 and 1L…
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Sodium Acetate
G-BiosciencesFeatures Synonym: Acetic acid sodium salt CAS#: 127-09-3 Molecular Formula: C2H3O2Na Molecular Weight: 82.03
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Acetate Buffer
G-BiosciencesAcetate Buffer at pH 5.0 and 5.5 are supplied as ready to use solutions that eliminates the calculation, formulation and weighing errors. Features Precision in pH Guaranteed reproducibility
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Tris HCl
G-BiosciencesFeatures Synonym: Tris(hydroxymethyl)aminomethanehydrochloride CAS#: 1185-53-1 Molecular Formula: C4H11NO3 · HCl Molecular Weight: 157.6
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IEF Cathode Buffer (pH 3-10) [10X]
G-BiosciencesIsoelectric Focusing Buffer for Cathode, pH3-10 Features pH 3-10
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Features Synonym: 1,4-Piperazinediethanesulfonic acid sesquisodium salt, Piperazine-1,4-bis(2-ethanesulfonic acid) sesquisodium salt CAS#: 100037-69-2 Molecular Formula: …
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IgG Binding, Wash & Elution Buffers
G-BiosciencesG-Biosciences IgG Binding, Wash & Elution Buffers are designed for the non-denaturing, high yield purification of antibodies from IgG affinity purification resins.The buffers are ideal for the purification of antibodies from Protein A , Protein G and Protein A/G resins. IgG Binding…
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RIPA Lysis & Extraction Buffer
G-BiosciencesA complete lysis buffer for the release of cytoplasmic, membrane and nuclear proteins from adherent and suspension cultured mammalian cells. The RIPA lysis buffer is fully compatible with many applications, including reporter assays, protein assays, immunoassays and other protein purification…
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STE Buffer [10X]
G-BiosciencesFeatures 100mM Tris.HCl (pH 8.0), 10mM EDTA, 1M NaCl Synonym: TEN Buffer
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Potassium Phosphate (Monobasic)
G-BiosciencesFeatures Synonym: Monopotassium phosphate CAS#: 7778-77-0 Molecular Formula: KH2PO4 Molecular Weight: 136.09
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Tris Glycine Native Gel Running Buffer
G-BiosciencesFeatures Tris-Glycine Native Running Buffer [10X]
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STM Buffer
G-BiosciencesFor growing cells and for competent cells preparation Features 270mM Sucrose, 10mM Tris-HCl, ph 7.5, 1mM MgCl2
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FOCUS™ Extraction Buffers
G-BiosciencesChaotropic extraction buffers that preserve the native charge of proteins making them s uitable for sample extraction for 2D gels One of the most important considerations before running 2D gel electrophoresis is the choice of protein solubilization buffers. A suitable buffer must…
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Tris-EDTA-NaCl Buffer, pH 8.0
G-BiosciencesTris-EDTA-NaCl Buffer solution at pH 8.0. A customer enquired about this buffer to make as custom and we also added as a new product. Features Precision in concentration and pH Guaranteed reproducibility Applications As a buffering mix
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STET Solution
G-BiosciencesBuffer for DNA/RNA extraction and purification. Features 8% w/v sucrose, 5% v/v Triton® X-100, 50mM EDTA, 50mM Tris-HCl, pH 8.0
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PBST [10X]; Phosphate buffered saline with Tween-20
G-BiosciencesA 10X Concentrated solution of Phosphate Buffered Saline with Tween® 20. The 10X Concentration is 80mM Na2HPO4, 1.5M NaCl, 20mM KH2PO4, 30mM KCl, 0.5% Tween® 20, pH 7.4. PBST is commonly used as a wash solution for Western blot…
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Sodium Phosphate (Dibasic)
G-BiosciencesFeatures Synonym: Disodium hydrogen phosphate CAS#: 7558-79-4 Molecular Formula: Na2HPO4 Molecular Weight: 231-448-7
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PBS (Phosphate Buffered Saline)
G-BiosciencesPBS (Phosphate Buffered Saline) Ready-to-use solutions, available in 1X and 10X concentrated solutions. Features 1X: 8mM NaH2PO4,150mM NaCl, 3mM KCl, 2mM KH2PO4(pH 7.4) 10X: 80mM NaH2PO4 1.5M NaCl, 30mM KCl, 20mM…
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Molecular Biology Universal Kit
G-BiosciencesA selection of 12 x 25ml molecular biology relevant buffers. These buffers are routinely used in molecular biology and are all DNase and RNase free. The buffers supplied are listed below. Features Molecular Biology Universal Kit Ammonium acetate [5M] Calcium chloride [1M] …
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Efficient™ Western Transfer Buffer
G-BiosciencesG-Biosciences scientists have developed a high efficiency transfer buffer for Western blotting. The pre-made and ready-to-use [20X] transfer buffer is prepared for optimal conductivity and efficient protein transfer without generating excessive heat or transfer distortion. Efficient ™ …
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SSC [20X]
G-BiosciencesSaline-Sodium Citrate HybridizationBuffer [20X] controls stringency during the washing steps in Southern blotting, insitu hybridization, DNA microarray or Northern blotting. 20X SSC may be used to prevent the drying of agarose gels during a vacuum transfer. Features 0.3M trisodium…
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TBST [10X]; Tris buffered saline with Tween-20
G-BiosciencesA 10X Concentrated solution of Tris Buffered Saline with Tween® 20. The 10X Concentration is 100mM Tris.HCl, 150mM NaCl, 0.5% Tween® 20 at pH7.5. The 1X Concentration is 10mM Tris.HCl, 15mM NaCl, 0.05% Tween® 20 at pH7.5. TBST…
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ACD (Acid Citrate Dextrose) Solutions
G-BiosciencesACD (Acid Citrate Dextrose) Solution A andACD (Acid Citrate Dextrose)Solution B are anticoagulants for whole blood and also act as acidifying agents to platelet poor plasma. Features Solution A: 38mM citric acid, 107mM sodium citrate, 136mM dextrose Solution B: 23mM citric acid,…
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STET-E1 Solution
G-BiosciencesFor DNA/RNA Extraction and Purification Features 3M NaCl, 165mM Tris-HCl, pH 8.0, 45mM EDTA, pH 8.0, and 16.4% Tween® 20
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Sodium Bicarbonate
G-BiosciencesFeatures Synonym: Natron CAS#: 144-55-8 Molecular Formula: NaHCO3 Molecular Weight: 84.01
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RAB Buffer (Reassembly Buffer)
G-BiosciencesA high salt RAB (Reassembly) buffer for the lysis of mammalian cells, including CHO 1,2 , COS 3 , NT2N 4,5 and HEK293 10 ; C. elegans 6,10 and brain tissue 7-9 . Designed for the extraction of soluble proteins, does not extract detergent extractable insoluble proteins. Features …
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TE Buffer [100X]
G-BiosciencesTE Buffer (Tris-EDTA Buffer) is a common molecular biology buffer that is used to protect DNA and RNA from degradation. The Tris buffering agent and EDTA metal chelating properties help protect DNA and RNA. Suitable for 10 liters of 1X TE Buffer Features Synonyms: TE Buffer,…