G-Biosciences Buffer Solutions
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Denhardt Solution [100X]
G-BiosciencesSuitable for nucleic acid hybridization. Denhardt's solution is a mixture of high-molecular weight polymers capable of saturating non-specific binding sites and artificially increasing the concentration of available probe. Features 2% Ficoll 400 0.3M sodium chloride 2% PVP…
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Phosphate Buffers
G-BiosciencesPhosphate buffers are buffer solutions commonly used in biological research. They are water based salt solution containing sodium phosphate monobasic and dibasic at a fixed pH. Competitive remarks and statements Feature statements Application statements Features Useful pH…
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PBS [10X] (Phosphate Buffered Saline)
G-BiosciencesPBS (Phosphate Buffered Saline) Ready-to-use solutions, available in 1X and 10X concentrated solutions. Features 1X: 8mM NaH2PO4, 150mM NaCl, 3mM KCl, 2mM KH2PO4 (pH 7.4) 10X: 80mM NaH2PO4 1.5M NaCl,…
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Tris-EDTA-NaCl Buffer, pH 8.0
G-BiosciencesTris-EDTA-NaCl Buffer solution at pH 8.0. A customer enquired about this buffer to make as custom and we also added as a new product. Features Precision in concentration and pH Guaranteed reproducibility Applications As a buffering mix
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Zymogram Sample Buffer
G-BiosciencesZymography is an electrophoretic technique, based on SDS-PAGE, that includes an enzyme substrate copolymerized with the polyacrylamide gel. Samples are prepared in Zymogram Sample Buffer without boiling to preserve the structure and activity of the enzyme. Following electrophoresis, the SDS is…
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Sodium Phosphate (Dibasic)
G-BiosciencesFeatures Synonym: Disodium hydrogen phosphate CAS#: 7558-79-4 Molecular Formula: Na2HPO4 Molecular Weight: 231-448-7
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SDS-PAGE Sample Loading Buffers
G-BiosciencesThe SDS-PAGE Sample Loading Buffers are suitable for loading protein samples on to the SDS-polyacrylamide gels. The buffers are provided in 2X and 6X concentrations containing Tris-HCl, glycerol, SDS and bromophenol blue (BPB) in recommended concentrations and is stable at room temperature. …
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STET-E1 Solution
G-BiosciencesFor DNA/RNA Extraction and Purification Features 3M NaCl, 165mM Tris-HCl, pH 8.0, 45mM EDTA, pH 8.0, and 16.4% Tween® 20
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JAW™ Tris-HEPES-SDS Buffer Packs
G-BiosciencesJAW™ Tris-HEPES-SDS Buffer Packs are ready to use dry-blend powder pouches used to make running buffer for SDS-PAGE with Tris-HEPES gels Features : Each pack when dissolved in deionized water and made to final volume of 500 ml makes 0.1M Tris, 0.1M HEPES, 3 mM SDS, pH 8 Come in…
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FOCUS™ Extraction Buffers
G-BiosciencesChaotropic extraction buffers that preserve the native charge of proteins making them s uitable for sample extraction for 2D gels One of the most important considerations before running 2D gel electrophoresis is the choice of protein solubilization buffers. A suitable buffer must…
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Modified Dulbecco's PBS Packs (for ELISA and Western Blots) are dry-blend powder pouches used to make physiological Sodium- and Potassium- buffer saline (D-PBS) for ELISA and Western blot diluents and wash buffers. Features : Each pouch when dissolved in DI water and made to final…
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SSC [20X]
G-BiosciencesSaline-Sodium Citrate HybridizationBuffer [20X] controls stringency during the washing steps in Southern blotting, insitu hybridization, DNA microarray or Northern blotting. 20X SSC may be used to prevent the drying of agarose gels during a vacuum transfer. Features 0.3M trisodium…
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Streptavidin Elution Buffer
G-BiosciencesFeatures 8M Guanidine.HCl, pH 1.5 Streptavidin resin also available
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JAW Dry Powder Buffer Packs
G-BiosciencesPremixed buffer powders, just add water to generate a ready-to-use buffer. JAW™ Carbonate-Bicarbonate Buffer packs make 1L of 0.2M Carbonate-Bicarbonate buffer, pH 9.4. JAW™ Phosphate Buffered Saline (PBS) packs [1X] make 1L of 2.7mM potassium chloride, 137mM sodium chloride…
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TBS [10X] (Tris buffered saline)
G-BiosciencesTBS, Tris buffered saline [10X] 100mM Tris HCl, 1.5M NaCl, pH 7.5
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High Molecular Weight Transfer Buffer [5X]
G-BiosciencesBased on Efficient ™ Western Transfer Buffer, the High Molecular Weight Transfer buffer is designed to facilitate the transfer of notoriously difficult high molecular weight proteins (>70kDa) during Western blotting. Supplied as 1 liter of a 5X concentrated solution.
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SDS-PAGE Running Buffer
G-BiosciencesFeatures SDS-PAGE Running Buffer (10X) Compatible with Tris-Glycine gels Separate proteins in the range 6-200 kDa under denaturing conditions 1 X concentration: 25mM Tris Base, 192 mM Glycine, 0.1% SDS Applications Tris-Glycine/SDS Running Buffer [10X] is compatible…
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IgG Binding, Wash & Elution Buffers
G-BiosciencesG-Biosciences IgG Binding, Wash & Elution Buffers are designed for the non-denaturing, high yield purification of antibodies from IgG affinity purification resins.The buffers are ideal for the purification of antibodies from Protein A , Protein G and Protein A/G resins. IgG Binding…
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Tris
G-BiosciencesTris is an abbreviation of the organic compound known as tris(hydroxymethyl)aminomethane, with the formula (HOCH2)3CNH2. Tris is extensively used in as a major component in buffer solutions. This 1M solution is has a pH of 9.0. Other 1M Tris solutions are…
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SSC [20X] (Sodium Chloride-Sodium Citrate)
G-BiosciencesSaline-Sodium Citrate Hybridization Buffer [20X] controls stringency during the washing steps in Southern blotting, in situ hybridization, DNA microarray or Northern blotting. 20X SSC may be used to prevent the drying of agarose gels during a vacuum transfer. Features …
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Tris Base
G-BiosciencesFeatures Synonym: 2-Amino-2-(hydroxymethyl)-1,3-propanediol, THAM, Tris base, Tris(hydroxymethyl)aminomethane, Trometamol Linear Formula: NH2C(CH2OH)3 Molecular Weight: 121.14 CAS Number: 77-86-1
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PBS (Phosphate Buffered Saline)
G-BiosciencesPBS (Phosphate Buffered Saline) Ready-to-use solutions, available in 1X and 10X concentrated solutions. Features 1X: 8mM NaH2PO4,150mM NaCl, 3mM KCl, 2mM KH2PO4(pH 7.4) 10X: 80mM NaH2PO4 1.5M NaCl, 30mM KCl, 20mM…
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JAW™ Borate Buffer Packs
G-BiosciencesJAW™ Borate Buffer Packs are dry blend powder pouches used to make borate buffer which is used in protein modification procedures that require amine free and alkaline pH conditions. Features : Each Pack is dissolved in 500 ml deionized water to get 50 mM borate buffer, pH8.5 Come…
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Tris HCl
G-BiosciencesFeatures Synonym: Tris(hydroxymethyl)aminomethanehydrochloride CAS#: 1185-53-1 Molecular Formula: C4H11NO3 · HCl Molecular Weight: 157.6
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STE Buffer [10X]
G-BiosciencesFeatures 100mM Tris.HCl (pH 8.0), 10mM EDTA, 1M NaCl Synonym: TEN Buffer
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TBE-Urea Sample Buffer
G-BiosciencesLoading buffer to resolve single-stranded DNA oligos or RNA into sharp, distinct bands. For the analysis and purification of products ranging from 20-800 bases, making them an ideal choice for synthetic oligo analysis and purification, RNase Protection Assays (RPA), in vitro transcription studies,…
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Efficient™ Western Transfer Buffer
G-BiosciencesG-Biosciences scientists have developed a high efficiency transfer buffer for Western blotting. The pre-made and ready-to-use [20X] transfer buffer is prepared for optimal conductivity and efficient protein transfer without generating excessive heat or transfer distortion. Efficient ™ …
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TBE [10X] (0.9M Tris-borate, 0.01M EDTA (pH8.3))
G-BiosciencesFeatures 0.9M Tris-borate, 0.01M EDTA (pH8.3)
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RBC Lysis Buffer
G-BiosciencesOur RBC Lysis Buffer is designed for the optimal lysis of erythrocytes, while having minimal effect of lymphocytes, when used as directed. The use of RBC Lysis Buffer allows for the preferential lysis of red blood cells from whole blood and as these are the majority of cells in whole blood…
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Tris Glycine Native Gel Running Buffer
G-BiosciencesFeatures Tris-Glycine Native Running Buffer [10X]