Buffers
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BLOTTO A PRE-MIXED, 500mL, Lyophilized
Rockland Immunochemicals IncUse BLOTTO whenever procedures call for immunoanalytical grade non-fat dry milk. Alternative blocking agents such as BLOTTO A (solution of TBS, 5% non-fat dry milk and 0.05% Tween-20) and BLOTTO B (solution of TBS, 1% non-fat dry milk, 1% BSA and 0.05% Tween-20) are also available. BLOTTO A is a…
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Revitablot™ Western Blot Stripping Buffer
Rockland Immunochemicals IncRevitablot™ Western Blot Stripping Buffer is ready-to-use as a working 1X solution and requires no further dilution.
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3.0 M Sodium Acetate pH 5.2 DEPC TREATED, 100mL, Liquid (sterile filtered)
Rockland Immunochemicals IncSodium Ethanoate is a concentrated stock solution and should be diluted appropriately with distilled, deionized water or equivalent to its final working concentration. This buffer contains 3.0 M Sodium Acetate at pH 5.2 meticulously prepared using ultra pure reagents dissolved in highly polished…
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X-GAL BETA-GALACTOSIDASE SUBSTRATE, 100mg, Solid
Rockland Immunochemicals IncX-Gal (5-Bromo-4-chloro-3-indolyl-ß-D-galactopyranoside) produces a dimethylformamide (DMF)-soluble, stable blue precipitate at the reaction site. X-Gal is the classical substrate used with ß-galactosidase in current methods of molecular biology, microbiology and immunology. X-Gal is used to…
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FETAL BOVINE SERUM Origin: United States triple 0.1 micron filtered
Rockland Immunochemicals IncFetal Bovine Sera sourced from the United States is collected exclusively from USDA licensed abattoirs from within the United States. Raw material is processed through 0.1 µm (100nm) filters in compliance with regulatory guidelines. Animals are inspected pre-mortem and post-mortem by USDA…
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10X PBS pH 7.2 (0.2 M Potassium Phosphate 1.5 M Sodium Chloride)
Rockland Immunochemicals IncThis product is a concentrated stock solution and should be diluted appropriately with distilled, deionized water or equivalent to its final working concentration. 10X Phosphate Buffered Saline (PBS) consists of 0.2 M Potassium Phosphate, 1.5 M Sodium Chloride, pH 7.2 prepared in highly polished…
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10X TBS pH 7.5 (1.0 M Tris HCl 1.5 M Sodium Chloride)
Rockland Immunochemicals IncThis product is a concentrated stock solution and should be diluted appropriately with distilled, deionized water or equivalent to its final working concentration. 10X Tris Buffered Saline consists of 1.0 M Tris HCl and 1.5 M Sodium Chloride, pH 7.5, meticulously prepared using ultra pure reagents…
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10% (w/v) Sodium Dodecyl Sulfate (SDS), 100mL, Liquid (sterile filtered)
Rockland Immunochemicals Inc10% SDS is a concentrated stock solution and should be diluted appropriately with distilled, deionized water or equivalent to its final working concentration. Product consists of 10% (w/v) Sodium Dodecyl Sulfate (SDS). Meticulously prepared using ultra pure reagents dissolved in highly polished…
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10X SDS-PAGE Running Gel Buffer (0.25 M Tris, 1.92 M Glycine, 1.0% SDS pH 8.3),…
Rockland Immunochemicals IncThis product is a 10X concentrated stock solution and should be diluted appropriately with distilled, deionized water (or equivalent) to its final working concentration. No pH adjustment is required.
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ProXtract B™ Extraction Buffer
Rockland Immunochemicals IncProXtract B™ Buffer is suitable for protein extraction and easily scalable. Specific conditions should be optimized by the end user. See protocol.
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Express Transfer Buffer, 1L, Liquid (sterile filtered)
Rockland Immunochemicals IncExpress Transfer Buffer is a alternative to tris-glycine as a transfer buffer in the western blot. It may run at an elevated temperature but gives markedly lower background.
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1.0 M Tris HCl pH 8.0, 1L, Liquid (sterile filtered)
Rockland Immunochemicals IncTris solution is suitable for molecular immunology, molecular biology and nucleic acid methodologies, when applicable. This product is a concentrated stock solution and should be diluted appropriately with distilled, deionized water or equivalent to its final working concentration. This buffer…
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1.0 M Magnesium Chloride DEPC TREATED, 100mL, Liquid (sterile filtered)
Rockland Immunochemicals IncThis product is a 1.0M concentrated stock solution and should be diluted appropriately with distilled, deionized water or equivalent to its final working concentration. This buffer contains 1.0 M Magnesium Chloride and was meticulously prepared using ultra pure reagents dissolved in highly…
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UltraPure Sterile Water (4 x 1 liter), 4L, Liquid (sterile filtered)
Rockland Immunochemicals IncSuitable for molecular immunology, molecular biology and nucleic acid methodologies, when applicable.
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Tris-Acetate-EDTA (TAE)
Rockland Immunochemicals IncTris-Acetate-EDTA (TAE) is a concentrated stock solution and should be diluted appropriately with distilled, deionized water or equivalent to its final working concentration. Tris-Acetate-EDTA (TAE) consists of 2.0 M Tris-Acetate, 0.05 M EDTA at a pH of 8.3. Meticulously prepared using ultra pure…
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1X TES pH 7.8 (10 mM Tris HCl, 100 mM NaCl, 1 mM EDTA) DEPC TREATED, 1L, Liquid …
Rockland Immunochemicals IncThis product is ready-to-use as a working 1X solution and requires no further dilution. This buffer consists of 10 mM Tris HCl, 100 mM NaCl, and 1.0 mM EDTA at a pH of 7.8. Meticulously prepared using ultra pure reagents dissolved in DEPC treated pharmaceutical grade deionized water.
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10% (w/v) Sodium Dodecyl Sulfate (SDS), 1L, Liquid (sterile filtered)
Rockland Immunochemicals IncThis product is a concentrated stock solution and should be diluted appropriately with distilled, deionized water or equivalent to its final working concentration. Product consists of 10% (w/v) Sodium Dodecyl Sulfate (SDS). Meticulously prepared using ultra pure reagents dissolved in highly…
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10X TTBS pH 7.5 (1.0 M Tris HCl 1.5 M Sodium Chloride 0.1% (v/v) Tween-20), 1L,…
Rockland Immunochemicals IncTBST is suitable for molecular immunology, molecular biology and nucleic acid methodologies, when applicable. May be used as a wash buffer for immunological assays including western blot, immunohistochemistry, immunofluorescence microscopy, and ELISA.
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FREUND'S COMPLETE ADJUVANT
Rockland Immunochemicals IncFreund’s adjuvant may be used to form water-in-oil emulsions with antigens for the injection of immunogens for antibody production. Proper emulsion formation is essential and should be tested by observing a drop of the emulsion on the surface of water which should 'bead and not disperse. Antigens…
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1X SDS-PAGE Destain Solution (Ready-to Use), 1L, Liquid
Rockland Immunochemicals Inc1X SDS-PAGE Destain Solution is ready-to-use as a working solution and requires no further dilution.
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Molecular Biology Grade UltraPure Water
Rockland Immunochemicals IncMolecular Biology Grade Ultrapure water is suitable for molecular immunology, molecular biology and nucleic acid methodologies, when applicable.
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10X TE pH 7.5 (100 mM Tris HCl 10 mM EDTA) DEPC TREATED, 1L, Liquid (sterile…
Rockland Immunochemicals IncThis product is a concentrated stock solution and should be diluted appropriately with distilled, deionized water or equivalent to its final working concentration. This buffer consists of 100 mM Tris HCl and 10 mM EDTA at a pH of 7.5. Meticulously prepared using ultra pure reagents dissolved in…
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20% (w/v) Sodium Dodecyl Sulfate (SDS)
Rockland Immunochemicals IncThis product is a concentrated stock solution and should be diluted appropriately with distilled, deionized water or equivalent to its final working concentration. Product consists of 20% (w/v) Sodium Dodecyl Sulfate (SDS). Meticulously prepared using ultra pure reagents dissolved in highly…
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Blocking Buffer for Fluorescent Western Blotting 3-PACK (3 x 500 ml), 1Each,…
Rockland Immunochemicals IncFluorescence technology is widely used to detect proteins in both the visible and near-infrared ranges. This product allows for superior signal detection and lower background noise when fluorochrome conjugated antibodies are used to visualize proteins in western blotting and other applications.…
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5.0 M Potassium Acetate (untitrated) DEPC TREATED
Rockland Immunochemicals IncThis product is a 5.0M concentrated stock solution and should be diluted appropriately with distilled, deionized water or equivalent to its final working concentration. This buffer contains untitrated 5.0 M Potassium Acetate. Meticulously prepared using ultra pure reagents dissolved in highly…
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10X TE pH 8.0 (100 mM Tris HCl 10 mM EDTA) DEPC TREATED, 1L, Liquid (sterile…
Rockland Immunochemicals IncTE buffer is a concentrated stock solution and should be diluted appropriately with distilled, deionized water or equivalent to its final working concentration. This buffer consists of 100 mM Tris HCl and 10 mM EDTA at a pH of 8.0. Meticulously prepared using ultra pure reagents dissolved in DEPC…
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1.0 M Tris HCl pH 7.4, 1L, Liquid (sterile filtered)
Rockland Immunochemicals IncTris HCl solution is suitable for molecular immunology, molecular biology and nucleic acid methodologies, when applicable. This buffer consists of 1.0 M Tris HCl at pH 7.4. This product is a concentrated stock solution and should be diluted appropriately with distilled, deionized water or…
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10X RIPA Lysis Buffer
Rockland Immunochemicals IncThis product is 10X concentrated stock solution. Dilute to 1X prior to use. 1X RIPA Lysis Buffer is intended for the extraction of cellular proteins for the efficient lysis of cells and solubilization of protein, while minimizing protein degradation and maintaining protein immunoreactivity and…
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Borate Buffered Saline (BBS) (10 mM Sodium Borate, 150 mM Sodium Chloride pH -8…
Rockland Immunochemicals IncBBS is isotonic and has a strong bactericidal effect. It can be used to dilute solutions for coating procedures. Additives such as Tween 20 and Blotto can be used to add functionality as a washing buffer or blocking buffer.
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1X TE pH 8.0 (10 mM Tris HCl, 1.0 mM EDTA) DEPC TREATED, 1L, Liquid (sterile…
Rockland Immunochemicals IncThis product is ready-to-use as a working 1X solution and requires no further dilution. This buffer consists of 10 mM Tris HCl and 1.0 mM EDTA at a pH of 8.0. Meticulously prepared using ultra pure reagents dissolved in DEPC treated pharmaceutical grade deionized water.
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Tris-Glycine
Rockland Immunochemicals IncTris-Glycine running buffer is suitable for laboratory involved in protein biochemistry. This product is a 10X concentrated stock solution and should be diluted appropriately with distilled, deionized water (or equivalent) to its final working concentration. No pH adjustment is required. 10X…
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10X PBST (0.2 M Potassium Phosphate 1.5 M Sodium Chloride, 0.5% (v/v) Tween-20,…
Rockland Immunochemicals IncThis product is a 10X concentrated stock solution and should be diluted appropriately with distilled, deionized water or equivalent to its final working concentration. Dilute only prior to immediate use. 10X Phosphate Buffered Saline (PBS) consists of 0.2 M Potassium Phosphate, 1.5 M Sodium…
