Buffers

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  • Components: Sodium Phosphate Monobasic / Sodium Phosphate Dibasic(0.5 M), Water(Rest) Method: Prepared in 18.2 megohms-cm ± 1 water and filtered through 0.22-micron filter. pH: 7.6 ± 0.15 Storage: Room Temp.

  • Components: Citric Acid / Sodium Citrate(0.5 M), Water(rest) Method: Prepared in 18.2 megohms-cm ± 1 water and filtered through 0.22-micron filter. pH: 6.5 ± 0.15 Storage: 2-8°C

  • Acetate Buffer (1 M, pH 5.0)

    Boston BioProducts

    Components: Acetic Acid / Sodium Acetate(1 M), Water(Rest) Method: Prepared in 18.2 megohms-cm ± 1 water and filtered through 0.22-micron filter. pH: 5.0 ± 0.15 Storage: Room Temp.

  • Components: Sodium Phosphate Monobasic / Sodium Phosphate Dibasic(500 mM), Water(Rest) Method: Prepared in 18.2 megohms-cm ± 1 water and filtered through 0.22-micron filter. pH: 8.0 ± 0.15 Storage: Room Temp.

  • Tris-HCl Buffer (1 M, pH 8.0)

    Boston BioProducts

    Components: Tris Base / Tris-HCl(1 M), Water(rest) Method: Prepared in 18.2 megohms-cm ± 1 water and filtered through 1-micron filter. pH: 8.0 ± 0.2 Storage: 2-8°C

  • Components: Tris Base / Tris-HCl(250 mM), Potassium Chloride(27 mM), Sodium Chloride(1.37 M) Method: Prepared in 18.2 megohms-cm ± 1 water and filtered through 1-micron filter. pH: 7.4 ± 0.15 (1X) Storage: Room Temp.

  • Components: Sodium Phosphate Monobasic(~2.4-2.9%), Sodium Phosphate Dibasic(~2.4-2.9%), Water(rest) Method: Prepared in 18.2 megohms-cm ± 1 water and filtered through 0.22-micron filter. pH: 7.6 ± 0.15 Storage: Room Temp.

  • RIPA Buffer

    Boston BioProducts

    Components: Tris Base / Tris-HCl(50 mM), Sodium Chloride(150 mM), Sodium Deoxycholate(0.5%), Sodium Dodecyl Sulfate (SDS)(0.1%), Nonidet P-40 Substitute(1%) Method: Prepared in 18.2 megohms-cm ± 1 water and filtered through 0.22-micron filter. pH: 7.4 ± 0.15 Storage: 2-8°C

  • Borate Buffer (0.5 M, pH 9.5)

    Boston BioProducts

    Components: Boric Acid(0.5 M) Method: Prepared in 18.2 megohms-cm ± 1 water and filtered through 0.22-micron filter. pH: 9.5 ± 0.15 Storage: Room Temp.

  • Citrate Buffer (0.5 M, pH 3.0)

    Boston BioProducts

    Components: Citric Acid / Sodium Citrate(0.5 M), Water(rest) Method: Prepared in 18.2 megohms-cm ± 1 water and filtered through 0.22-micron filter. pH: 3.0 ± 0.15 Storage: 2-8°C

  • Components: Sodium Phosphate Monobasic(~2.4-2.8%), Sodium Phosphate Dibasic(~2.4-2.8%), Water(rest) Method: Prepared in 18.2 megohms-cm ± 1 water and filtered through 0.22-micron filter. pH: 9.0 ± 0.15 Storage: Room Temp.

  • Components: Boric Acid(0.5 M) Method: Prepared in 18.2 megohms-cm ± 1 water and filtered through 0.22-micron filter. pH: 9.0 ± 0.15 Storage: Room Temp.

  • Components: Citric Acid / Sodium Citrate(0.5M) Method: Prepared in 18.2 megohms-cm ± 1 water and filtered through 0.22-micron filter. pH: 5.5 ± 0.15 Storage: 2-8°C

  • Components: Citric Acid / Sodium Phosphate Dibasic(100 mM / 200 mM mixture), Water(rest) Method: Prepared in 18.2 megohms-cm ± 1 water and filtered through 0.22-micron filter. pH: 7.0 ± 0.15 Storage: 2-8°C

  • Components: Sodium Phosphate Monobasic / Sodium Phosphate Dibasic(0.2 M), Water(Rest) Method: Prepared in 18.2 megohms-cm ± 1 water and filtered through 0.22-micron filter. pH: 6.8 ± 0.15 Storage: Room Temp.

  • Components: Potassium Phosphate Monobasic(200 mM), Potassium Phosphate Dibasic(200 mM), Water(rest) Method: Prepared in 18.2 megohms-cm ± 1 low endotoxin water and filtered through 0.22-micron filter. pH: 7.2 ± 0.15 Storage: Room Temp.

  • Components: Citric Acid / Sodium Citrate(0.5 M) Method: Prepared in 18.2 megohms-cm ± 1 water and filtered through 0.22-micron filter. pH: 6.0 ± 0.15 Storage: 2-8°C

  • Components: Citric Acid / Sodium Phosphate Dibasic(100 mM / 200 mM mixture), Water(rest) Method: Prepared in 18.2 megohms-cm ± 1 water and filtered through 0.22-micron filter. pH: 6.5 ± 0.15 Storage: 2-8°C

  • HEPES Buffer (1 M, pH 7.4)

    Boston BioProducts

    Components: HEPES(1 M), Sodium Hydroxide(-), Water(Rest) Method: Prepared in 18.2 megohms-cm ± 1 water and filtered through 0.22-micron filter. pH: 7.4 ± 0.15 Storage: 2-8°C

  • Components: EGTA(0.5 M), Water(rest) Method: Prepared in 18.2 megohms-cm ± 1 water and autoclaved at 15 psi (121°C) for 30 minutes on liquid cycle. pH: 8.0 ± 0.15 Storage: Room Temp.

  • Components: Citric Acid / Sodium Citrate(0.5 M), Water(rest) Method: Prepared in 18.2 megohms-cm ± 1 water and filtered through 0.22-micron filter. pH: 5.0 ± 0.15 Storage: 2-8°C

  • Citrate Buffer (0.5 M, pH 5.5)

    Boston BioProducts

    Components: Sodium Citrate / Citric Acid(0.5 M), Water(rest) Method: Prepared in 18.2 megohms-cm ± 1 water and filtered through 0.22-micron filter. pH: 5.5 ± 0.15 Storage: 2-8°C

  • Components: Acetic Acid / Sodium Acetate(1 M), Water(rest) Method: Prepared in 18.2 megohms-cm ± 1 low endotoxin water and filtered through 0.22-micron filter. pH: 4.0 ± 0.15 Storage: Room Temp.

  • Borate Buffer (0.5 M, pH 8.5)

    Boston BioProducts

    Components: Boric Acid(0.5 M), Sodium Hydroxide(for pH adjustment), Water(rest) Method: Prepared in 18.2 megohms-cm ± 1 water and filtered through 0.22-micron filter. pH: 8.5 ± 0.15 Storage: Room Temp.

  • Bicine Buffer (0.5 M, pH 8.5)

    Boston BioProducts

    Components: Bicine(500 mM), Sodium Hydroxide(For pH adjustment) Method: Prepared in 18.2 megohms-cm ± 1 water and filtered through 0.22-micron filter. pH: 8.5 ± 0.15 Storage: 2-8°C

  • Components: Tris Base / Tris-HCl(10 mM), EDTA(1 mM), Water(rest) Method: Prepared in 18.2 megohms-cm ± 1 water and autoclaved at 15 psi (121°C) for 30 minutes on liquid cycle and/or filtered through 0.22-micron filter. pH: 7.6 ± 0.15 Storage: Room Temp.

  • Components: Tris Base / Tris-HCl(1 M), Water(Rest) Method: Prepared in 18.2 megohms-cm ± 1 water and filtered through 0.22-micron filter. pH: 8.2 ± 0.2 Storage: 2-8°C

  • PIPES Buffer (1 M, pH 6.8)

    Boston BioProducts

    Components: PIPES(1 M), Water(Rest) Method: Prepared in 18.2 megohms-cm ± 1 water and filtered through 0.22-micron filter. pH: 6.8 ± 0.15 Storage: 2-8°C

  • RIPA Buffer (with EGTA)

    Boston BioProducts

    Components: Tris Base / Tris-HCl( 50 mM), Sodium Chloride(150 mM), Nonidet P-40 Substitute(1%), Sodium Deoxycholate(0.5%), EGTA(1 mM), Sodium Dodecyl Sulfate (SDS)(0.1%), Water(Rest) Method: Prepared in 18.2 megohms-cm ± 1 water and filtered through 0.22-micron filter. pH: 7.4 ±…

  • Components: Sodium Phosphate Monobasic / Sodium Phosphate Dibasic(0.2 M), Water(Rest) Method: Prepared in 18.2 megohms-cm ± 1 water and filtered through 0.22-micron filter. pH: 7.5 ± 0.15 Storage: Room Temp.

  • MES Buffer (0.5 M, pH 8.0)

    Boston BioProducts

    Components: MES Monohydrate(500 mM), Water(rest) Method: Prepared in 18.2 megohms-cm ± 1 water and filtered through 0.22-micron filter. pH: 8.0 ± 0.15 Storage: 2-8°C

  • Components: Potassium Phosphate Monobasic / Potassium Phosphate Dibasic(0.2 M), Water(Rest) Method: Prepared in 18.2 megohms-cm ± 1 water and filtered through 0.22-micron filter. pH: 7.2 ± 0.15 Storage: Room Temp.<

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