Buffers

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  • AMP Buffer (0.2 M, pH 10.5)

    Boston BioProducts

    Components: AMP(200 mM), Water(rest) Method: Prepared in 18.2 megohms-cm ± 1 water and filtered through 0.22-micron filter. pH: 10.5 ± 0.15 Storage: 2-8°C

  • Components: Tris Base / Tris-HCl(250 mM), Sodium Chloride(1.37 M), Potassium Chloride(27 mM), Tween-20(0.5%), Sodium Azide(0.02%), Water(rest) Method: Prepared in 18.2 megohms-cm ± 1 water and autoclaved at 15 psi (121°C) for 30 minutes on liquid cycle. pH: 7.4 ± 0.15 (1X) …

  • Components: Sodium Carbonate / Sodium Bicarbonate(1 M), Water(rest) Method: Prepared in 18.2 megohms-cm ± 1 water and filtered through 0.22-micron filter. pH: 8.0 ± 0.15 Storage: Room Temp.

  • Components: Tris Base / Tris-HCl(500 mM), Sodium Chloride(1.5 M) Method: Prepared in 18.2 megohms-cm ± 1 water and filtered through 1-micron filter. pH: 7.4 ± 0.15 (1X) Storage: Room Temp.

  • Components: Tris Base / Tris-HCl(0.5 M), Sodium Chloride(1.5 M), Tween-20(1%), Kathon(0.02%), Water(rest) Method: Prepared in 18.2 megohms-cm ± 1 water and autoclaved at 15 psi (121°C) for 30 minutes on liquid cycle. pH: 7.6 ± 0.15 (1X) Storage: Room Temp.

  • Components: Sodium Phosphate Monobasic Monohydrate / Sodium Phosphate Dibasic Heptahydrate(20 mM), Water(rest) Method: Prepared in 18.2 megohms-cm ± 1 low endotoxin water and filtered through 0.22-micron filter. pH: 6.5 ± 0.15 Storage: Room Temp.

  • Components: Tris Base / Tris-HCl(1 M), Sodium Chloride(3 M), Water(rest) Method: Prepared in 18.2 megohms-cm ± 1 water and filtered through 1-micron filter. pH: 7.4 ± 0.15 (1X) Storage: Room Temp.

  • Components: HEPES(125 mM), Sodium Chloride(750 mM), Water(rest) Method: Prepared in 18.2 megohms-cm ± 1 water and filtered through 0.22-micron filter. pH: 6.5 ± 0.15 (1X) Storage: Room Temp.

  • Maleate Buffer (0.2 M, pH 6.5)

    Boston BioProducts

    Components: Maleate(200 mM), Water(rest) Method: Prepared in 18.2 megohms-cm ± 1 water and filtered through 0.22-micron filter. pH: 6.5 ± 0.15 Storage: Room Temp.

  • Tris-HCl Buffer (0.5 M, pH 9.0)

    Boston BioProducts

    Components: Tris Base / Tris-HCl(0.5 M), Water(Rest) Method: Prepared in 18.2 megohms-cm ± 1 water and filtered through 0.22-micron filter. pH: 9.0 ± 0.2 Storage: 2-8°C

  • Components: Sodium Phosphate Dibasic(10 mM), Potassium Phosphate Monobasic(2 mM), Potassium Chloride(2.7 mM), Sodium Chloride(137 mM), Triton X-100(0.1%), Water(Rest) Method: Prepared in 18.2 megohms-cm ± 1 water and filtered through 0.22-micron filter. pH: 7.4 ± 0.15 …

  • Components: Tris Base / Tris-HCl(250 mM), Potassium Chloride(27 mM), Sodium Chloride(1.37 M), Water(rest) Method: Prepared in 18.2 megohms-cm ± 1 water and filtered through 1-micron filter. pH: 7.6 ± 0.15 (1X) Storage: Room Temp.

  • MOPS Buffer (0.2 M, pH 6.0)

    Boston BioProducts

    Components: MOPS(~4.2%), Water(rest) Method: Prepared in 18.2 megohms-cm ± 1 water and filtered through 0.22-micron filter. pH: 6.0 ± 0.15 Storage: 2-8°C

  • RIPA Buffer (without SDS)

    Boston BioProducts

    Components: Tris Base / Tris-HCl(50 mM), Sodium Chloride( 150 mM), NP-40(1% ), Sodium Deoxycholate(0.25%), Water(Rest) Method: Prepared in 18.2 megohms-cm ± 1 water and filtered through 0.22-micron filter. pH: 7.4 ± 0.15 Storage: 2-8°C

  • Components: Potassium Phosphate Monobasic / Potassium Phosphate Dibasic(0.2 M), Water(Rest) Method: Prepared in 18.2 megohms-cm ± 1 water and filtered through 0.22-micron filter. pH: 7.2 ± 0.15 Storage: Room Temp.<

  • Components: HEPES(1 M), Potassium Hydroxide(-), Water(Rest) Method: Prepared in 18.2 megohms-cm ± 1 water, pH adjusted with KOH and filtered through 0.22-micron filter. pH: 7.4 ± 0.15 Storage: 2-8°C

  • Citrate Buffer (0.5 M, pH 4.0)

    Boston BioProducts

    Components: Citric Acid / Sodium Citrate(0.5 M), Water(rest) Method: Prepared in 18.2 megohms-cm ± 1 water and filtered through 0.22-micron filter. pH: 4.0 ± 0.15 Storage: 2-8°C

  • Components: EGTA(0.5 M), Water(rest) Method: Prepared in 18.2 megohms-cm ± 1 water and autoclaved at 15 psi (121°C) for 30 minutes on liquid cycle. pH: 8.0 ± 0.15 Storage: Room Temp.

  • HEPES Buffer (0.5 M, pH 8.0)

    Boston BioProducts

    Components: HEPES(500 mM), Sodium Hydroxide(for pH adjustment), Water(Rest) Method: Prepared in 18.2 megohms-cm ± 1 water and filtered through 0.22-micron filter. pH: 8.0 ± 0.15 Storage: 2-8°C

  • Tris-HCl Buffer (1 M, pH 7.0)

    Boston BioProducts

    Components: Tris Base / Tris-HCl(1 M), Water(rest) Method: Prepared in 18.2 megohms-cm ± 1 water and filtered through 1-micron filter. pH: 7.0 ± 0.2 Storage: 2-8°C

  • Components: Sodium Phosphate Monobasic / Sodium Phosphate Dibasic(1 M), Water(Rest) Method: Prepared in 18.2 megohms-cm ± 1 water and filtered through 0.22-micron filter. pH: 8.0 ± 0.15 Storage: Room Temp.

  • Components: MOPS(~10.5%), Water(rest) Method: Prepared in 18.2 megohms-cm ± 1 water, pH adjusted with KOH and filtered through 0.22-micron filter. pH: 6.5 ± 0.15 Storage: 2-8°C

  • Components: Sodium Chloride(3.0 M), Tween-20(2%), Sodium Azide(0.02%), Tris Base / Tris-HCl(1 M) Method: Prepared in 18.2 megohms-cm ± 1 water and autoclaved at 15 psi (121°C) for 30 minutes on liquid cycle. pH: 7.4 ± 0.15 (1X) Storage: Room Temp.

  • Components: Tris Base / Tris-HCl(1M), Sodium Chloride(3 M), Tween-20(2%), Kathon(0.02%) Method: Prepared in 18.2 megohms-cm ± 1 water and autoclaved at 15 psi (121°C) for 30 minutes on liquid cycle. pH: 7.4 ± 0.15 (1X) Storage: Room Temp.

  • Glycine Buffer (0.2 M, pH 2.5)

    Boston BioProducts

    Components: Glycine(200 mM), Hydrochloric Acid(for pH adjustment), Water(rest) Method: Prepared in 18.2 megohms-cm ± 1 water and filtered through 0.22-micron filter. pH: 2.5 ± 0.15 Storage: 2-8°C

  • Bicine Buffer (0.5 M, pH 7.5)

    Boston BioProducts

    Components: Bicine(500 mM), Water(rest) Method: Prepared in 18.2 megohms-cm ± 1 water and filtered through 0.22-micron filter. pH: 7.5 ± 0.15 Storage: 2-8°C

  • Imidazole Buffer (1 M, pH 7.0)

    Boston BioProducts

    Components: Imidazole(1 M), Hydrochloric Acid(for pH adjustment), Water(rest) Method: Prepared in 18.2 megohms-cm ± 1 water and filtered through 0.22-micron filter. pH: 7.0 ± 0.15 Storage: 2-8°C

  • Components: Sodium Phosphate Dibasic(100 mM), Potassium Phosphate Monobasic(20 mM), Potassium Chloride(27 mM), Sodium Chloride(1.37 M), Tween-20(1%), Water(rest) Method: Prepared in 18.2 megohms-cm ± 1 water and autoclaved at 15 psi (121°C) for 30 minutes on liquid cycle. pH: 7.6…

  • HEPES Buffer (1 M, pH 7.0)

    Boston BioProducts

    Components: HEPES(1 M), Sodium Hydroxide(-), Water(Rest) Method: Prepared in 18.2 megohms-cm ± 1 water and filtered through 0.22-micron filter. pH: 7.0 ± 0.15 Storage: 2-8°C

  • Components: Citric Acid / Sodium Citrate(0.2 M), Water(rest) Method: Prepared in 18.2 megohms-cm ± 1 low endotoxin water and filtered through 0.22-micron filter. pH: 5.0 ± 0.15 Storage: 2-8°C

  • Citrate Buffer (0.5 M, pH 3.0)

    Boston BioProducts

    Components: Citric Acid / Sodium Citrate(0.5 M), Water(rest) Method: Prepared in 18.2 megohms-cm ± 1 water and filtered through 0.22-micron filter. pH: 3.0 ± 0.15 Storage: 2-8°C

  • Components: Potassium Phosphate Monobasic / Potassium Phosphate Dibasic(200 mM) Method: Prepared in 18.2 megohms-cm ± 1 water and filtered through 0.22-micron filter. pH: 7.0 ± 0.15 Storage: Room Temp.

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