Immobilized Metal Ion Affinity Chromatography (IMAC), developed by Porath et al (1), is based on the interaction of certain protein residues (histidines, cysteines, and to some extent tryptophans) with cations of transition metals. The Copper Chelating Resin is specifically designed for the…

Features 8M Guanidine.HCl, pH 1.5 Streptavidin resin also available

G-Biosciences scientists have developed a high efficiency transfer buffer for Western blotting. The pre-made and ready-to-use [20X] transfer buffer is prepared for optimal conductivity and efficient protein transfer without generating excessive heat or transfer distortion. Efficient ™ …

Ficin (or Ficain) is a cysteine protease enzyme (EC 3.4.22.3) isolated from fig latex is that has the endopeptidase activity to cleave immunoglobulin G molecules in the hinge reason.Ficin is typically used to cleave mouse IgG₁ as this are difficult to cleave with papain and pepsin .In…

An interesting hands-on lab activity that teaches students the skills required for the isolation of bacteria from test samples. This kit teaches aseptic handling techniques and cultivation of bacteria.   Using bacterial culture techniques, students discover and isolate the bacteria…

Features   Synonym: Diethylpyrocarbonate CAS#: 1609-47-8 Molecular Formula: C₆H₁₀O₅ Molecular Weight: 162.14

( Bis (2-[Succinimidooxycarbonyloxy]ethyl) sulfone Features Molecular Weight: 436.35 Spacer Arm (Å): 13 Reactive Toward: Primary Amines Membrane Permeable: YES Water Soluble: NO Cleavable/ Reversible: Base

G-Trap™ ProteinG are prepacked ready to use columns for affinity purification of Immunoglobulins G. The column is packed with resin, which is covalently coupled to recombinant Protein G. Recombinant Protein G is coupled covalently by proprietary coupling method to highly crosslinked 6%…

Features CAS # 7447-40-7 Chemical Formula KCl Molecular Weight 74.55 g mol -1

MW: 544.43 C₂₂H₂₉N₇O₅ · 2HCl Mode of action and Anti-microbial spectrum: Puromycin is a protein synthesis inhibitor that causes premature chain termination by acting as an analog of the 3′-terminal end of aminoacyl-tRNA. It prevents…

1,4 Dithiothreitol (DTT) is a small molecule reducing agent, also known as Cleland’s reagent. G-Biosciences DTT is a high quality proteomic grade DTT and is supplied in various sizes, including a OneQuant™ version. The OneQuant™ format features 40 vials of 7.7mg DTT for convenient…

X-Sulfate potassium salt; 5-Bromo-4-chloro-3-indoxyl sulfate, potassium salt MOLECULAR BIOLOGY GRADE X-Sulfate K is a slightly off-white to faint-yellowish powder that is used as a chromogenic substrate for aryl-sulfatase. X-Sulfate K yields a blue precipitate when cleaved. Store at…

An ELISA kit for the detection of ROMO1 (Human). This uses Sandwich ELISA, Double Antibody and has a sensitivity of 18.75pg/ml.

A Chemically Modified, TPCK treated, Affinity Purified Trypsin Trypsin is a serine endopeptidase that specifically cleaves peptide bonds on the carboxy side of s-aminoethyl cysteine, arginine and lysine residues, and typically, there is little to no cleavage at arginyl-proline and lysyl-proline…

ECH-Agarose contains carboxyl functional group for coupling of affinity ligand s via their amine groups for affinity chromatography. ECH-Agarose is prepared by condensation of Carboxyl Coupling Resin (Cat. # 786-797) with succinic anhydride, resulting in 15-atom hydrophilic spacer arm. Amine…

 A 10X Concentrated solution of Tris Buffered Saline with Tween® 20.    The 10X Concentration is 100mM Tris.HCl, 150mM NaCl, 0.5% Tween® 20 at pH7.5.   The 1X Concentration is 10mM Tris.HCl, 15mM NaCl, 0.05% Tween® 20 at pH7.5.   TBST…

DNAmark™ 100bp Ladder consists of 11 fragments ranging from 100-1500 base pairs (bp). The 500bp fragment is present at increased intensity to allow easy identification. All fragments are precisely quantified and mixed during the production. For 5µl loading, all fragments except 500bp…

An ELISA kit for the detection of JUP (Human). This uses Sandwich ELISA, Double Antibody and has a sensitivity of 0.094ng/ml.

The Carbohydrate Coupling Resin is designed for the simple and efficient coupling of glycoproteins to a solid agarose support through oxidized sugar groups. The coupling is through the hydrazide group that is immobilized to agarose beads. The resin is ideal for immobilized polyclonal antibodies as…

An ELISA kit for the detection of EPO (Mouse). This uses Sandwich ELISA, Double Antibody and has a sensitivity of 28.125pg/ml.

This kit is designed to teach 8 groups of four students the principles of enzyme assays.   The kit contains the reagents to allow students to test for the presence and activity of 2 different types of amylase by: Visualizing the breakdown of the starch substrate by the amylase…

Conventional stain used for Hematoxylin and Eosin (H&E) staining in histology and cytology procedures. Ready to use formulation optimized for histology and cytology procedures where reproducible results are important. Manufactured with BSC certified reagents.

EndotoxinOUT™ was designed for the rapid removal of endotoxins and pyrogens (LPS, lipopolysaccharides) from nucleic acid (DNA) samples. EndotoxinOUT™ is an ideal product for the cleanup of buffers, cell culture media, protein solutions, nucleic acid (DNA) samples and pharmacological…

An ELISA kit for the detection of PLVAP (Human). This uses Sandwich ELISA, Double Antibody and has a sensitivity of 0.094ng/ml.

Mode of Action: G-418 inhibits protein elongation by blocking polypeptide synthesis. Eukaryotic cells that are stably transfected with neomycin resistance genes can be selected and maintained using this product. Similar in structure to Gentamicin, Neomycin, and Kanamycin. Store dessicated at…

A CCL6 (C-C motif chemokine 6) ELISA with a senstivity of 9.375pg/ml and a range of 15.625-1000pg/ml. Uses the detection method Sandwich ELISA, Double Antibody

Agarose I has low electroendosmosis (EEO) and high electrophoresis mobility.  Suitable for nucleic acid analytical and preparative electrophoresis; blotting; and protein electrophoresis (i.e. Radial Immunodiffusion).   Specifications Moisture: ≤ 7% Ash: 4% EEO: …

An ELISA kit for the detection of SEPT5 (Human). This uses Sandwich ELISA, Double Antibody and has a sensitivity of 0.469ng/ml.

The premade blots are prepared with the appropriate GenLysate and 50μg protein is loaded onto a 4-20% denaturing polyacrylamide gel, along with a prestained molecular weight marker. After the proteins are resolved they are transferred to a PVDF membrane, using Efficient Western Transfer…

Tested and Certified for in vivo imaging Luciferin is a common bioluminescent reporter used for in vivo imaging of the expression of luciferase. This water soluble substrate for the Firefly luciferase enzyme utilizes ATP and Mg2+ as co-factors to emit a characteristic yellow-green emission in…

Agarose II has a low melting and gelling temperature compared to standard agaroses.  The low melting temperature allows for the recovery of undamaged nucleic acids below the denaturation temperature.  The low gelling temperature ensures that the agarose will be in a liquid state at a…

Church & Gilbert’s Hybridization Buffer is prepared from molecular biology grade SDS, monosodium phosphate, EDTA and BSA Fraction V using DNase, RNase and Protease-free water. Features 1mM EDTA(ethylenediaminetetracetic acid) 1% BSA (bovine serum albumin) 0.5M…