Western Blotting and ELISA Chemicals
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picoLUCENT™ PLUS-HRP Kit
G-BiosciencespicoLUCENT™ PLUS-HRP kit is based on our ultra sensitive Luminol substrate that produces chemiluminescence upon reaction with horseradish peroxidase. The chemiluminescence light emission can be recorded by a short exposure to autoradiography films. picoLUCENT™ PLUS-HRP reagents are…
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Low Fluorescence Western Membrane (LF PVDF) 0.45um (9x7)
Azure BiosystemsPre-cut LF PVDF membrane (9x7 cm mini vertical gels); 10 sheets
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NBT/BCIP Stain Kit
Bio Basic Inc.NBT/BCIP Stain Kit: NBT and BCIP refers to Nitro-Blue-Tetrazolium and 5-bromo-4-chloro-3-indolyl-phosphate respectively. When mixed together, BCIP is hydrolyzed and reacts with NBT, which then react with O2, forming visible purple precipitates in the presence of AP (alkaline phosphatase). The…
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NAP-BLOCKER™
G-BiosciencesA major drawback of current blocking solutions, such as BSA , casein and milk powder, is that they are derived from animal sources. The presence of animal proteins can often lead to high non-specific backgrounds as antigens and antibodies, generated in animals, interact with the…
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Swift™ Western Blotting System
G-BiosciencesThe Swift™ Western Blotting System is a unique system that reduces the blocking and antibody incubations on Western blot membranes from >4 hours to <60 minutes. Using a combination of proprietary wash and diluent buffers and our highly sensitive femtoLUCENT™ …
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Atezolizumab Elisa Kit
Lab BioreagentsAtezolizumab ELISA Kit is a sandwich enzyme-linked immunoassay for the quantitative determination of Atezolizumab with high sensitivity and specificity in serum and plasma. Kit Performance Sensitivity: 10 ng/mL Assay Range: 10 – 1000 ng/mL Cross-reactivity: There is no cross reaction…
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Well-Coated™ Streptavidin Plates
G-BiosciencesWell-Coated ™ Streptavidin plates are designed to specifically bind biotinylated molecules, including biotin tagged antibodies.This is particular advantageous for antibodies known to denature upon direct binding to polystyrene plates. Biotin exhibits an extraordinary binding affinity for…
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SWIFT™ Transfer Pads
G-BiosciencesWestern blot analysis of proteins is a routine and commonly used technique in research laboratories, with 3 major drawbacks. The first is the amount of time taken to transfer the proteins to a protein binding membrane; the second is the variable efficiency of the transfer and the third is problems…
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Azure Fluorescent Blot Washing Buffer
Azure BiosystemsWestern blot washing solution for use with fluorescent secondary antibodies, 10x concentrate; 500 ml
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Nitrocellulose Transfer Membrane 0.22um (8x10)
Azure BiosystemsPre-cut membrane (8x10 cm mini vertical gels, ideal for smaller proteins); 10 sheets
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femtoCHROMO™-HRP Kit
G-Biosciencesfemto CHROMO™-HRP kit is supplied with a single step chromogenic substrate for the rapid development of protein band s with low background staining. To ensure low background staining, each kit is supplied with an enhanced blocking agent, BLOT-QuickBlocker™, and a concentrated (10X)…
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Rituximab Elisa Kit
Lab BioreagentsRituximab ELISA Kit is a sandwich enzyme-linked immunoassay for the quantitative determination of Rituximab with high sensitivity and specificity in serum and plasma. Kit Performance Sensitivity: 10 ng/mL Assay Range: 10 – 10,000 ng/mL Cross-reactivity: There is no cross reaction with…
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0.83mm thickness, smooth surface Economical - compare to GB003 Generally used as a blotter under the "weight" of a capillary sandwich. Assures continuous contact of buffer from the chamber through the gel and transfer membrane to the opposite pole (electroblot) or weight…
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AzurePonceau Stain
Azure BiosystemsVisible reversible total protein stain for proteins transferred to membrane – sufficient for staining 50 blots 9x7 cm; 500 ml
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OptiBlaze™ ELISA
G-BiosciencesStabilized ultra sensitive luminol and 1,2 dioxetane based horseradish peroxidase or alkaline phosphatase substrate for the detection of HRP or AP-conjugated antibodies during ELISA. OptiBlaze™ ELISA femto-HRP or OptiBlaze™ ELISA femto-AP are chemiluminescent detection systems for…
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FLASHBlot™ Transfer Buffers
AdvanstaEnhanced protein transfer for improved sensitivity Achieve improved detection of low-abundance and posttranslationally modified proteins with Advansta's proprietary FLASHBlot Transfer Buffer. High-efficiency protein transfer and increased protein retention on the membrane add up to more…
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Nitrocellulose Transfer Membrane 0.45um (8x10)
Azure BiosystemsPre-cut membrane (8x10 cm mini vertical gels, ideal for larger proteins); 10 sheets
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Radiance Q
Azure BiosystemsQuantitative HRP substrate for CCD (150 ml kit); contains substrate for 1,500 cm2 membrane
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HRP Stripping Buffer
Azure BiosystemsUniformly removes antibodies from developed HRP Western blots so the blot can be reprobed with another set of antibodies; 500 ml
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100% pure cotton fiber; 0.345mm thickness Sheets are smooth, flat and RNase free Economical - compare to 3MM Our HyBlot 3A™ paper is specifically designed, controlled and packaged for electrophoresis and blotting applications. High purity grade cotton fiber paper is…
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Flash Western Demo Kit – Rabbit
Azure BiosystemsKit for testing the chemiluminescent Flash Western blot protocol – includes all reagents necessary for testing the Flash Western protocol including control lysate, molecular weight marker, LF PVDF membrane, control primary antibody, sample buffer, transfer buffer, blocking buffer, HRP conjugate,…
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AzureSpectra Labeling Kit – for Western Blot – 650
Azure BiosystemsKit for covalently labeling antibodies for Western blot with a fluorescent dye – includes labeling buffer, dye solution, quenching solution, neutralization buffer and two G-25 columns – each kit includes sufficient reagents to perform two labelings of 50 micrograms of antibody
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AzureSpectra RG rb650/ms550 Western Kit, with Fluorescent Block
Azure BiosystemsKit contains materials for 10 Western blots – LF PVDF membranes, wash buffer, fluorescent blocking buffer, secondary antibodies AzureSpectra rb650/ms550
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Incubation Trays
AdvanstaConvenient, easy to use trays for staining and washing gels and membranes These specially designed incubation trays are ideal for staining and washing electrophoresis gels and membranes. Chose from three designs, all available in a range of sizes: Opaque – for light-sensitive…
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Radiance Plus
Azure BiosystemsFemtogram HRP substrate (150 ml kit); contains substrate for 1,500 cm2 membrane
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Chemiluminescent substrate for ELISA applications ELISABright is an enhanced and highly sensitive chemiluminescent HRP substrate optimized for chemiluminescent ELISA applications. This luminol-based substrate demonstrates a wide linear dynamic range and a superior signal to noise ratio.…
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AzureSpectra Labeling Kit – for Western Blot – 490
Azure BiosystemsKit for covalently labeling antibodies for Western blot with a fluorescent dye – includes labeling buffer, dye solution, quenching solution, neutralization buffer and two G-25 columns – each kit includes sufficient reagents to perform two labelings of 50 micrograms of antibody
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AzureSpectra RG rb650/ms550 Western Kit, with Protein-Free Block
Azure BiosystemsKit contains materials for 10 Western blots – LF PVDF membranes, wash buffer, protein-free blocking buffer, secondary antibodies AzureSpectra rb650/ms550
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Trastuzumab Elisa Kit
Lab BioreagentsTrastuzumab ELISA Kit is a sandwich enzyme-linked immunoassay for the quantitative determination of Trastuzumab with high sensitivity and specificity in serum and plasma. Kit Performance Sensitivity: 10 ng/mL Assay Range: 10 – 300 ng/mL Cross-reactivity: There is no cross reaction…
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Azure Transfer Buffer
Azure BiosystemsTransfer buffer (50x concentrated solution) sufficient for 50-100 blots; 500 ml
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Low Fluorescence Western Membrane (LF PVDF) 0.45 um (13x18)
Azure BiosystemsPre-cut LF PVDF membrane (13x18 cm vertical gels); 10 sheets
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Low Fluorescence Western Membrane (LF PVDF) 0.45um (10x15)
Azure BiosystemsPre-cut LF PVDF membrane (10x15 cm vertical gels); 10 sheets