Dyes & Stains, Electrophoresis, Detection

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Dyes & Stains, Electrophoresis, Detection

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  • DAPI

    Biotium

    DAPI is a popular blue fluorescent DNA dye. The dye binds to the minor groove of dsDNA with approximately 20-fold fluorescence enhancement.

  • Propidium iodide (PI) is a membrane-impermeant nucleic acid intercalator. The dye is commonly used to selectively stain dead cells in a cell population and also used as a counterstain in multicolor fluorescent imaging.

  • Acridine Orange

    Biotium

    Acridine orange (AO) stains dsDNA green (525 nm) and RNA or single stranded DNA red (650 nm).The dye is membrane-permeant and its nucleic acid binding property has been used for cell-cycle studies.

  • X-Gal

    IBI Scientific

    X-GAL is histochemical substrate for β-galactosidase, allowing the detection of lac gene expression. To make a stock solution of X-GAL, dissolve the X-GAL in dimethylformamide to make a 20mg/ml solution. Note: Use either a glass or polypropylene tube. Add 4ml of this solution per liter of…

  • EvaGreen® dye is a green fluorescent nucleic acid dye with features that make the dye useful for several applications including qPCR and DNA melt curve analysis, real-time monitoring of thermophilic helicase-dependent amplification (tHDA), and capillary gel electrophoresis.

  • IPTG

    IBI Scientific

    IPTG is an inducer of β-galactosidase activity in E. coli. It is commonly used in conjunction with X-GAL to detect lac gene expression in cloning applications, thus allowing detection of recombinant molecules.

  • 6X Loading Dye

    IBI Scientific

    IBI’s ready-to-use 6X loading dye is ideally suited for agarose electrophoresis of DNA, RNA, or nucleic acids. Its ultra pure grade ensures quality and repeatibility every time you use it. The light blue, indigo, and magenta dyes will clearly show the migration of each run, and the dyes…

  • PMA (propidium monoazide) dye is a DNA modifier invented by scientists at Biotium. It is a photo-reactive DNA-binding dye that is useful for determination of microbial viability by qPCR.

  • AdvanStain Ponceau rapidly detects proteins on nitrocellulose and PVDF membranes, allowing you to check the quality of protein transfer before proceeding to Western blotting. With AdvanStain Ponceau, you can quickly make sure that protein transfer has been even across the entire blot, and that no…

  • Ethidium Bromide

    IBI Scientific

    Ethidium Bromide can be used to detect both single and double stranded nucleic acids (both DNA and RNA). However, Ethidium Bromide does not intercollate into single-stranded DNA very well; therefore, the fluorescence of single-stranded DNA is relatively poor in comparison to double-stranded DNA.…

  • Rapid : 15 minute, non-toxic, safe, 1-step stain. No organic solvents! No phosphoric acid! Sensitive : 50 times more sensitive  than other rapid stains. Lower limit is 5 ng protein standard. Linear Range : Very low background enabling accurate quantitation of proteins. High…

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