Cloning and Expression

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Cloning and Expression

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  • Culture Cylinders

    Bioptechs

    A borosilicate glass cylinder similar to a cloning ring having an optically flat polished end that hydrostatically seals to another flat optical surface so that grease is not required that is used when plating cells to position them for observation and improve plating time by restricting the amount…

  • Single Step Ultra Competent Cell Preps Kit: This Kit contains SSCS soluon, enables researchers to prepare competent E. coli cells in a single step and to transform the cells without heat-shock. SSCS has been reported to be faster and easier than other methods of producing competent cells, such as…

  • MDH-2N1 is a direct replacement for PDHC-N01The Millicell® disposable plastic hemocytometer is used to manually count cells. It consists of a surface-patterned two enclosed chambers with two ports for sample injection. Count cells with the in the same way as you do with the Neubauer "improved"…

  • High efficiency transformation of yeast cells Process takes less than 90 minutes The Alkali Cation Yeast transformation kit provides all the reagents required to perform a high efficiency transformation of yeast with circular or linear DNA. The entire procedure can be completed in one day and…

  • Seamless cloning Master Mix (Kits): The Seamless Cloning Master Mix is a ready-to-use reaction mix for seamless cloning reactions. It allows for successful assembly of multiple DNA fragments, regardless of fragment length or end compatibility. It has been rapidly adopted by the…

  • Rosetta™ 2 host strains are BL21 derivatives designed to enhance the expression of eukaryotic proteins that contain codons rarely used in E. coli. These strains supply tRNAs for 7 rare codones (AGA, AGG, AUA, CUA, GGA, CCC, and CGG) on a compatible chloramphenicol-resistant plasmid. The tRNA…

  • PUCM-T Cloning Vector: The T-Vector PCR Product Cloning Kit is suitable for cloning of PCR products with additional A at 3’ end

  • pUCM-T Cloning Vector Kit

    Bio Basic Inc.

    pUCM-T Cloning Vector Kit: The T-Vector PCR Product Cloning Kit is suitable for cloning of PCR products with additional A at 3’ end. The special ligation system provided by the kit enables customs to finish ligation in 1-2 hour. pUCm-Tvector of our company isdesign for simplifing cloning of…

  • SUPER-COMP is an extremely rich medium that significantly increases the competency of the cells when using a simple RbCl/CaCl means of preparing competent host cells. After cells are made competent, they are immediately ready for DNA uptake. For maximum transformation efficiency, store for…

  • Yeast RPM Kit

    MP Biomedicals

    Plasmid DNA isolated from yeast or bacterial cells in ≤30 minutes Proven GENECLEAN® GLASSMILK® technology No phenol/chloroform extractions No ethanol precipitation The Yeast RPM Kit allows isolation of plasmid DNA from both yeast and bacterial cells. It uses a GLASSMILK purification…

  • Activates various cell types including B lymphocytes, macrophages, and dendritic cells by mimicking a viral infection For stimulation of interferon production in vitro Supplied as a lyophilized powder Since it was discovered that the double-stranded polyribonucleotide…

  • Sob & Soc Media

    MP Biomedicals

    SOB Medium is the standard media for preparing cells for chemical transformation.  SOC is a special medium for incubating competent cells immediately after transformation to allow expression of transferred resistance genes before exposing cells to selective conditions.

  • IPTG (Isopropyl-β-D-thiogalactopyranoside) is a galactose analogue not recognized by β-galactosidase. IPTG is a non-metabolizable galactose analog that induces expression of the lac operon in Escherichia coli. IPTG is a galactose analogue not recognized by β-galactosidase. It…

  • High throughput transformation of yeast cells Simple and fast No need to make competent cells The EZ-Yeast Transformation Kit is designed for high throughput transformation with an efficiency of up to 1,000 colonies per µg plasmid DNA. Suitable for a quick analysis of potentially positive…

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