G-Biosciences’ Amine Magnetic Beads are 1µm, uniformmagnetic beads with an amine (-NH2) surface functional group. The magnetic beads consist of a single-crystal Fe3O4 sphere core and dextran coating layer. Through chemical modification of dextran, the primary amino group (-NH2) are joined to the magnetic beads through a short hydrophilic linker. The hydrophilic surface ensures the magnetic beads excellent dispersion ability and easy hand ling property in a wide variety of buffers.
The magnetic beads with surface-reactive amino groups allow immobilization of ligand s such as proteins, peptides, carbohydrates or other target specific molecules.Immobilization of ligand s can be through reductive amination of aldehyde or ketones without prior activation of the bead surface.Alternatively, carbodiimide cross-linkers can be used to couple ligand s to the amines via their carboxyl groups.Finally, amine reactive heterobifunctional cross-linkers can be used to introduce other functional groups for coupling ligand s.
Carbodiimide activation of carboxyl groups produces a very labile intermediate that hydrolyzes quickly, meaning the ligand needs to be added rapidly.Alternatively, a two step protocol using N-hydroxysuccinimide (NHS) can be employed to produce a less labile intermediate that reacts over a longer time period.
Features
- 1µm beads
- ~50mM ligand density
- Rapid binding at neutral to high pH
- Compatible with a large range of amine reactive cross-linking reagents
- No centrifugations required
- Dextran coating for low non-specific binding
Applications
- Isolation of proteins and peptides
- C-terminal coupling of peptides