p-Iodonitrotetazolium Violet is used as a electron acceptor for the colorimetric assay of various dehydrogenases.

Pure Canavalia ensiformis lectin (Con A) from Jackbean. Isolated by affinity chromatography on cross-linked dextran. Con A exists as a dimer below pH 5.0 and and at pH >7 it exists as a tetramer. Con-A is not a glycoprotein. The monomeric molecular weight of Con-A is 25,500. Con-A does not…

Hyaluronidase is a glycoprotein containing 5% mannose and 2.17% glucosamine, it catalyzes the random hydrolysis of 1,4-linkages between 2-acetamido- 2-deoxy- b-D-glucose and D-glucose residues in hyaluronate. Hyaluronidase from bovine testes is a tetramer consisting of 4 equal subunits with a…

Dexamethasone is an anti-inflammatory glucocorticoid that induces the production of phospholipase A2 inhibitory protein (lipocortin). It also inhibits induction of nitric oxide synthase (IC50=5 nM). Dexamethasone has been shown to cause reduction in cyclin A and Cdk2 activity, inhibition of G1/S…

The molecule is a dialdehyde, consisting of two formyl (CHO) groups attached to adjacent carbon centres on a benzene ring. o-Phthalaldehyde is used for precolumn derivatization of amino acids for HPLC separation and for flow cytometric measurements of protein thiol groups. Used for fluorometric…

Vitamin K is a group of structurally similar, fat-soluble vitamins. Vitamin K1 is used as a cofactor in the formation of coagulation factors II (prothrombin), VII, IX and X; anticoagulant factors protein C and S and as a cell signaling factor.

Collagenase is a protease which cleaves the triple-helical protein called collagen. There are three types of tissue collagenases, and these belong to the matrix metalloproteinases (MMP) family. Collagenase from Clostridium histolyticum has extensive use in biological studies, where it is used to…

2,3,5-Triphenyltetrazolium Chloride is a redox indicator. In the TTC assay (also known as TTC test or tetrazolium test), TTC is used to differentiate between metabolically active and inactive tissues. The white compound is enzymatically reduced to red TPF (1,3,5-triphenylformazan) in living tissues…

Naloxone is specific and competitive opiate antagonist at mu, kappa, delta and sigma receptors; it blocks the action of sigma-agonists at opioid sites. It displaces morphine and other opiate agonists from the opiate receptor. It is more potent than Nalorphine and is also an effective antagonist for…

CHAPS is a nondenaturing zwitterionic detergent for solubilizing membrane proteins and breaking protein-protein interactions. Combines the useful properties of both the sulfobetaine-type and the bile salt detergents. CHAPS is commonly used for protein solubilization in isoelectric focusing and…

Trypsin consists of a single chain polypeptide of 223 amino acid residues. Member of the serine protease family Dissolves blood clots in its microbial form Treats inflammation in its pancreatic form Trypsin cleaves peptides on the C-terminal side of lysine and arginine residues.…

Erythromycin is a macrolide antibiotic that bind to the 50S ribosomal subunit and inhibit protein synthesis. This is an antimicrobial substance produced by the growth of certain strains of Streptomyces erythreus. It is a mixture consisting largely of erythromycin A with lesser amounts of…

D-Biotin is a growth factor present in small amounts in every living cell. It is involved in naturally occurring carboxylation reactions. It occurs mainly bound to proteins or polypeptides. It is more abundant in the liver, kidney, pancreas, yeast and milk. Biotin levels are higher in cancerous…

N-Acetyl-D-galactosamine(GalNAc), an aminosugar, is a component of many O-linked and N-linked glycan structures. Intial O-linked sugar to many serine and threonine residues in protein glycosylations.

Norethindrone is a synthetic progestin that possesses antiestrogenic effects, where in low doses it is progestational and in high doses it is antiestrogenic. It also causes a time-dependent loss of cytochrome P-450 when incubated in vitro with rat liver microsomal fractions and NADPH-generating…

β-estradiol is a potent mammalian estrogenic hormone produced by the ovary. Triggers the production of gonadotropins leading to ovulation. β-Estradiol is used to study cell differentiation and transformations (tumorigenicity). The pharmacology and HPLC determination of β-Estradiol…

Lysozyme (muramidase) hydrolyzes preferentially the β-1,4 glucosidic linkages between N-acetylmuramic acid and N-acetylglucosamine which occur in the mucopeptide cell wall structure of certain microorganisms, such as Micrococcus lysodeikticus. Extracts group specific antigens …

Collagenases degrade native helical collagen fibrils. Plays an important role in connective tissue metabolism Produced by specific cells involved in repairs and remodeling processes Type II enzyme that contains greater clostripain activity Collagenase is used for collagen…

n-Octyl-β-D-Glucopyranoside is a non-ionic detergent for membrane protein. n-Octyl-glucoside is a non-ionic alkyl-glucoside detergent commonly used in the solubilization of membrane proteins and phospholipids. n-Octyl-glucoside has been used at a molar dependant ratios for the…

o-Nitrophenyl-β-D-galactoside (ONPG) is a colorimetric substrate for b-galactosidase. This product is hydrolyzed to o-nitrophenol by the enzyme. o-Nitrophenol is yellow in alkaline solution and can be quantitated at 410-420 nm 2 . o-Nitrophenyl-β-D-galactoside is a cell culture…

5-Bromo-2’-deoxyuridine is a thymidine analog and useful for the study of DNA synthesis where it is incorporated into DNA in place of thymidine. Measure DNA synthesis Label dividing cells Study cell signaling and processes that induce cell proliferation Used as a mutagen in…

Cresyl violet acetate is a high purity biological stain to used for Nissl staining of spinal cord and brain paraffin sections and to stain mast cell and cartilage granules. Cresyl violet acetate is used for staining neve tissue and nerve cells and fresh turmor tissue for biopsy.

Oleic acid is a mono-unsaturated omega-9 fatty acid created by dehydrogenation of stearic acid. It is then further dehydrogenated and elongated to create members of the n-9 family of fatty acids. Oleic acid is used as emollient. Small amounts of oleic acid are used as an excipient in…

Rifamycin SV is a broad-spectrum semisynthetic antimicrobial agent of the rifamycin group. Rifamycin SV is used to study potential genes, such as rif15 and rif16, which may be involved in the conversion of rifamycin SV into rifamycin B. It is a potential treatment for traveler’s diarrhea…

D-Biotin is a growth factor present in small amounts in every living cell. Involved in carboxylation reactions Occurs mainly bound to proteins or polypeptides Abundant in the liver, kideny, pancreas, yeast, and milk Levels are higher in cancerous tumors than in normal tissues …

Adenosine 5′-diphosphate is a P2Y receptor agonist. Adenosine 5′-diphosphate has been used to study the release, neuronal effects and removal of extracellular β-nicotinamide adenine dinucleotide (β-NAD+) in the rat brain. Adenosine 5′-diphosphate has also been used to…

Melatonin is a hormone of the pineal gland. Hormone; mediates photoperiodicity in mammals; inhibits cerebellar nitric oxide synthetase; peroxynitrite scavenger. Melatonin has complex effects on apoptotic pathways, inhibiting apoptosis in immune cells and neurons but enhancing apoptotic cell…

Pullulan, edible plastic excellent in adhesivenss and film formability. Pullulan is a natural neutral polysaccharide produced from starch by cultivating a black yeast. Pullulan has been used in a study to assess bone tissue engineering. It has also been used in a study that investigated anti-fog applications.

Ribonuclease belongs to pancreatic ribonuclease family. RNase A is an endoribonuclease that attacks at the 3¢ phosphate of a pyrimidine nucleotide. The sequence of pG-pG-pC-pA-pG will be cleaved to give pG-pG-pCp and A-pG. Ribonuclease A is used to remove RNA from DNA plasmid preparations…

Retinoic acid (RA) is a vitamin A-derived, non-peptidic, small lipophilic molecule that mediates the functions of vitamin A required for growth and development. It affects gene transcription and modulates a wide variety of biological processes like Cell Proliferation, Differentiation, including…

Histamine Diphosphate Monohydrate is potent vasodilater. Endogenous H1 and H2 histamine receptor agonist; H1 activation mobilizes Ca2+; H2₂ activation stimulates adenylate cyclase activity in neurons; activates nitric oxide synthetase; potent vasodilator.

Fluorescent Brightner 28 is a colorless organic compound that fluoresces (clear bluish) under ultraviolet radiation. It is related structurally to Fluorescent Brightner 9 (Calcofluor White MR) which was used by Jefferies and Belcher and Maeda and Ischida in plant tissue studies. Fluorescent…