Trypsin consists of a single chain polypeptide of 223 amino acid residues.
- Member of the serine protease family
- Dissolves blood clots in its microbial form
- Treats inflammation in its pancreatic form
Trypsin cleaves peptides on the C-terminal side of lysine and arginine residues. The rate of hydrolysis of this reaction is slowed if an acidic residue is on either side of the cleavage site and hydrolysis is stopped if a proline residue is on the carboxyl side of the cleavage site. Trypsin can also act to cleave ester and amide linkages of synthetic derivatives of amino acids. EDTA is added to trypsin solutions as a chelating agent that neutralizes calcium and magnesium ions that obscure the peptide bonds on which trypsin acts. Removing these ions increases the enzymatic activity.
Grade: 2× Crystallized Specific Activity: =180 TAME u/mg
Key Applications: Dissolve blood clots
Application Areas: Molecular biology
Product Type: Proteins, Enzymes & Peptides
Components: Trypsin is composed of two subunits, α-trypsin and β-trypsin. α-Trypsin is composed of two peptide chains and β-trypsin is composed of one chain.
Biochemical Category: Proteins, Enzymes & Peptides
Chemical Class: Proteins, Enzymes & Peptides
Extinction Coefficient (E1%): E1%280 = 14.3 (Lit.)
Presentation: White Powder
Format: Lyophilized Powder
Isoelectric point (pI): pH 10.5(Lit.)
pH: Optimum pH: Approximately 8.0(Lit.)
NOTES: Inhibitors: Trypsin is inhibited by organophosphorus compounds such as diisopropyl fluorophosphate and natural trypsin inhibitors from pancreas, soybean, lima bean and egg white. Silver ions are also potent inhibitors. Specific inhibitors are AEBSF, antipain, aprotinin, DFP, leupeptin, PMSF, TLCK, and Trypsin Inhibitor.
Specificity: The protease activity of trypsin is highly specific toward positively charged side chains with lysine and arginine. Forms complexes with a2-macroglobulin. Can be used in the isolation of intact, detergent-free phycobilisomes and in the hydrolysis/condensation of carboxylic ester bonds.
Solubility: Soluble in water(50,000 u/mL); practically insoluble in alcohol and glycerol.
Storage & Handling: +4°C