o-Nitrophenyl-β-D-galactoside (ONPG) is a colorimetric substrate for b-galactosidase. This product is hydrolyzed to o-nitrophenol by the enzyme. o-Nitrophenol is yellow in alkaline solution and can be quantitated at 410-420 nm2.
o-Nitrophenyl-β-D-galactoside is a cell culture component for molecular genetics. It is chromogenic substrate for b-galactosidase and hence is useful in the detection of lacZ activity.
β-galactosidase, breaks down lactose into galactose and glucose. β-Galactosidase is not lactose specific and can act on simple galactosides. 2-Nitrophenyl β-D-galactopyranoside hydrolysis results in the release of galactose and a yellow chromogenic compound. The test substrate does not depend on an induced or constitutive permease enzyme to enter the cell, therefore reactions are rapid and occur within a 24-hour period.
Key Applications: Substrate for β-galactosidase
Application Areas: Cell Biology & Analysis
Product Type: Biochemicals
Biochemical Category: Inhibitors and substrates
Chemical Class: Carbohydrates
Melting Point: 190 - 202°C
Optical Rotation: -66 ° ± 7 ° (c=1, H2O)
UV/Visible Absorbance: 262 ± 3 nm λ max (H2O)
Presentation: White Solid
Format: Crystalline
Solubility: Soluble in water (10 mg/mL- clear, colorless solution; 15 mg/mL - clear, faint yellow solution; 20 mg/mL with heat - clear, yellowish solution).
Storage & Handling: Store at + 4°C.