Ribonuclease belongs to pancreatic ribonuclease family. RNase A is an endoribonuclease that attacks at the 3¢ phosphate of a pyrimidine nucleotide. The sequence of pG-pG-pC-pA-pG will be cleaved to give pG-pG-pCp and A-pG.
Ribonuclease A is used to remove RNA from DNA plasmid preparations and protein samples. It is also used in RNA sequence analysis and protection assays.
Ribonuclease A cleaves single stranded RNA after pyrimidine nucleotides.
Chromatographically Purified.
Grade: Molecular Biology Reagent
Specific Activity: ≥70 Kunitz units/mg
Key Applications: Purification of DNA, Analysis of RNA sequences, RNase protection assays, Hydrolyze RNA contained in protein samples
Application Areas: Genomics, Molecular Biology
Product Type: Proteins, Enzymes & Peptides
Extinction Coefficient (E1%): E1%280 = ~7.3
Presentation: White Powder
Format: Lyophilized powder, Salt-free and protease-free
Isoelectric point (pI): 9.45
pH: Optimum pH: 7.0 - 7.5
NOTES:
Inhibitors: Ribonuclease is inhibited by heavy metal ions and is competitively inhibited by DNA. The effect of denatured DNA is much greater than that of the native nucleic acid.
Composition: The composition and structure of ribonuclease A has been extensively investigated. The tertiary structure indicates that flexibility in a three-dimensional structure is essential for optimal catalytic activity.
Specificity: The enzyme is specific for pyrimidine nucleoside linkages, i.e., those cleaving cyclic 2',3'-pyrimidine nucleotide residues either singly or at the termination of purine nucleotide chains.
Solubility: Dissolves readily at 2 mg/mL in analytical grade water to give a clear colorless solution.
Storage & Handling: -20°C, desiccate