α-Amylase isolated from porcine pancreas is a glycoprotein. It is a single polypeptide chain of ~475 residues containing two SH groups and four disulfide bridges and a tightly bound Ca2+ necessary for stability. Chloride ions are necessary for activity and stability. α-Amylase is…

α-Thrombin from Bovine Plasma is highly specific serine protease. Thrombin is the final coagulation protease in regard to hemostasis, promoting both procoagulant and anticoagulant effects. Thrombin is used for site specific cleavage of recombinant fusion proteins containing an accessible…

Protease and Phosphatase Inhibitor Cocktail contains a mixture of inhibitors and is designed to prevent protein degradation by endogenous proteases and phosphatases released during the extraction of proteins from animal cells or tissues. Application Notes: Dilute 1/10 in RIPA or Lysis Buffer…

Choline oxidase (EC 1.1.3.17) is an enzyme that catalyzes the chemical reaction Choline + O2 betaine aldehyde + H2O2. Thus, the two substrates of this enzyme are choline and O2, whereas its two products are betaine aldehyde and H2O2. This enzyme belongs to the family of oxidoreductases,…

Anticancer compound. Chemotherapeutic used in patients with cancer and advanced forms of Kaposi's sarcoma. Microtubule assembly stabilizer. Reversibly binds to polymerized tubulin. Anti-mitotic. Mitotic spindle assembly, chromosome segregation and cell division inhibitor. Induces cell cycle…

Cell permeable, potent and selective class IIb HDAC6 inhibitor (IC50 =5nM). Displays high selectivity over all other HDACs (IC50=0.2-11µM). Induces cellular alpha-tubulin, but not histone H3 hyperacetylation in Neuro-2a cells. Promotes mitochondrial transport. Shows improved kinetics and…

Fungus-Derived Proteinase K Purified Recombinant is prepared at 20 mg/mL in 10 mM Tris-HCl, 1 mM calcium acetate, pH 7.5 containing 50% glycerol. This Molecular Biology Grade Proteinase K solution is extremely effective on native proteins and can be used to rapidly inactivate endogenous nucleases…

Cellulase refers to a group of enzymes which, acting together, hydrolyze cellulose. The b-glucosidases, including cellobiose, are primarily active on the smaller molecular weight cellulose hydrolysates. During cellulose breakdown they are active on the dimers and oligomers of cellulose. …

This enzyme is useful for enzymatic determination of creatinine when coupled with creatine amidinohydrolase, sarcosine dehydrogenase or sarcosine oxidase and formaldehyde dehydrogenase in clinical analysis. Creatininase from Pseudomonas sp. is a homohexameric enzyme with a molecular mass of…

Phosphorylation and dephosphorylation of proteins controls the processes of cell differentiation, proliferation, signal transduction, and apoptosis. Covalent binding of phosphate groups can change the conformation of proteins and the binding capacity of ligands. During protein extraction from…

Catalase, Fungal suspension from from Aspergillus niger is long-acting, extremely stable form of catalase that is active over a wide pH range. Composed of four protein subunits Each subunit contains a heme group bound to its active site Catalase is used for the removal of peroxides,…

HRP is a plant glycohemoprotein that belongs to the ferroprotoporphyrin group of peroxidases. HRP is composed of seven isozymes. All isozymes contain photohemin IX as prosthetic group. Neutral and amino sugars account for approximately 18% of the enzyme. The iron-containing hemin group is…

Elastase is an enzyme from the class of proteases (peptidases) that break down proteins. It hydrolyzes peptide bonds, especially those adjacent to neutral amino acids. Hydrolyses elastin Digests hemoglobin, casein, and fibrin One unit will solublize 1 mg of elastin in 20 minutes at…

Ribonuclease belongs to pancreatic ribonuclease family. RNase A is an endoribonuclease that attacks at the 3¢ phosphate of a pyrimidine nucleotide. The sequence of pG-pG-pC-pA-pG will be cleaved to give pG-pG-pCp and A-pG. Ribonuclease A is used to remove RNA from DNA plasmid preparations…

Yeast lytic enzyme is used to digest the cell wall of active yeast cells. Yeast lytic enzyme is an enzyme preparation from a submerged culture of Arthrobacter luteus which effectively lyses cell walls of viable yeast cell. It is a lyophilized enzyme partially purified by affinity chromatography.…

Iodoacetamide is a thiol reagent; alkylating reagent for cysteine and histidine residues in proteins. In the alkylation reaction, it reacts with histidine (such as in RNase), methionine and sulfhydryl groups of many proteins. Iodoacetamide can react with low molecular weight thiol compounds such as…

Papain is a sulfhydryl protease from Carica papaya latex. (A second protease, chymopapain, and a lysozyme have also been isolated from this same source.) Since native crystalline papain is quite unreactive until acted upon by mild reducing agents such as cysteine or cyanide, it may exist as a…

Protease from Staphylococcus aureus strain V8 is composed of a single polypeptide chain. The Staphylococcus strain V8 protease specifically cleaves peptide bonds on the carboxyl (COOH) terminal side of aspartic and glutamic acid residues. Protease V8 is used for selective cleavage of proteins…

Oleoyl Coenzyme A Dipotassium Salt

Lactase is an enzyme preparation produced by Aspergillus oryzae fermentation. Hydrolyzes Lactose Produces β-D-Galactose and a-D-Glucose One unit will hydrolyze 1.0 µmole of o-nitrophenyl-beta-D-galactoside to o-nitrophenol per minute at pH 4.5 and 30°C …

Ethylenediamine Tetraacetic Acid is a polyamino carboxylic acid hexadentate ligand and a chelating agent. Ethylenediamine Tetraacetic Acid is a chelator of divalent cations. It also inhibits enzymes, such as metalloproteases, that require divalent cations for activity. Zinc-dependent…

2,2'-Azino-bis-(3-ethylbenzthiazoline-6-sulfonic Acid) Solution is oxidized to a radical action showing absorption maxima at 820 nm, 734 nm, 650 nm and 405 nm, in the presence of hydrogen peroxide and HRP. The latter frequency demonstrates a significant molar extinction coefficient and is…

Glucose oxidase is an FAD-containing glycoprotein. The enzyme is specific for β-D-glucose. O can be replaced by hydrogen acceptors such as 2,6-dichlorophenol indophenol. Glucose oxidase from Aspergillus niger is a dimer consisting of 2 equal subunits with a molecular mass of 80 kDa each. Each…

Collagenases degrade native helical collagen fibrils. The substrate, collagen, is the major fibrous component of animal extracellular connective tissue: skin, tendon, blood vessels, bone, etc. True collagenase may cleave simultaneously across all three chains or attack a single strand. The enzyme…

Pancreatin is a mixture of several digestive enzymes produced by the exocrine cells of the porcine pancreas and delivered to the small intestine for the hydrolysis of complex nutrients. It is a broad-spectrum protease composed of amylase, trypsin, lipase, ribonuclease and protease. Pancreatin…

Trypsin consists of a single chain polypeptide of 223 amino acid residues. Member of the serine protease family Dissolves blood clots in its microbial form Treats inflammation in its pancreatic form Trypsin cleaves peptides on the C-terminal side of lysine and arginine residues.…

Hyaluronidase is a glycoprotein containing 5% mannose and 2.17% glucosamine, it catalyzes the random hydrolysis of 1,4-linkages between 2-acetamido- 2-deoxy- b-D-glucose and D-glucose residues in hyaluronate. Hyaluronidase from bovine testes is a tetramer consisting of 4 equal subunits with a…

Phosphoglucose Isomerase(PGI) is an enzyme crucial for the interconversion of D-glucose 6 phosphate and D-fructose 6-phosphate. Responsible for the second step of glycolysis Involved in glucogenesis Highly conserved in bacteria and eukaryotes Phosphoglucose Isomerase fuctions as an…

Cell permeable mycotoxin. Actin polymerization inhibitor. Binds to the barbed end of actin, reversibly inhibiting the elongation and shortening of actin filaments. Induces nuclear extrusion. By disrupting actin polymerization, blocks diverse cellular functions, including cell division, migration,…

Acetyl-CoA is produced via beta-oxidation of fatty acids, via the metabolism of carbohydrates - glucose 6-phosphate to pyruvate to acetyl-CoA and via the catabolism of amino acids. Acetyl-CoA has a number of metabolic opportunities. It is metabolized in the tricarboxylic acid cycle to produce…

Collagenase is a protease which cleaves the triple-helical protein called collagen. There are three types of tissue collagenases, and these belong to the matrix metalloproteinases (MMP) family. Collagenase from Clostridium histolyticum has extensive use in biological studies, where it is used to…