Yeast lytic enzyme is used to digest the cell wall of active yeast cells.
Yeast lytic enzyme is an enzyme preparation from a submerged culture of Arthrobacter luteus which effectively lyses cell walls of viable yeast cell. It is a lyophilized enzyme partially purified by affinity chromatography. The primary enzyme in this preparation responsible for lysis of viable yeast cells is β-1,3-glucan laminaripentaohydrolase. It hydrolyzes glucose polymers at the β-1,3-glucan linkages releasing laminaripentaose as the principal product.
Yeast Lytic Enzyme hydrolyzes poly-β(1→3)-glucose such as yeast cell wall glucan.
One unit will produce a delta A800 of 0.001 per minute at pH 7.5 and 25°C, using a suspension of brewers yeast as a substrate in a 3 mL reaction mixture.
Specific Activity: 20,000 u/g
Key Applications: Lysis of yeast cells
Application Areas: Molecular biology
Product Type: Proteins, Enzymes & Peptides
Presentation: Tan Powder
Format: Powder
pH: Optimum pH and temperature: pH 7.5, 35°C for lysis of viable yeast cells; pH 6.5, 45° for hydrolysis of yeast glucan.
pH Range: Stable through pH range 5 - 10(Lit.)
NOTES: Essential Enzyme:β-1,3-glucan laminaripentaohydrolase. Other Enzymes Present:β-1,3-glucanase, ca. 1.0 x 107 units/g. Protease, ca. 1.7 x 104 units/g. Mannase, ca. 6.0 x 104 units/g. DNase, RNase (none detectable).
Activators: Dithiothreitol, 2-mercaptoethanol and other sulfhydryl compounds (such as cystein).
Specificity: The lytic spectrum of this enzyme includes organisms from the following genera: Ashbya, Candida, Debaryomyces, Eremothecium, Endomyces, Hansenula, Hanseniaspora, Kloekera, Kluyveromyces, Lipomyces, Metschikowia, Pichia, Pullularia, Torulopsis, Saccharomyces, Saccharomcopsis, Saccharomycodes, Schwanniomyces and others.
Storage & Handling -20°C.