SUPER-COMP is an extremely rich medium that significantly increases the competency of the cells when using a simple RbCl/CaCl means of preparing competent host cells. After cells are made competent, they are immediately ready for DNA uptake. For maximum transformation efficiency, store for…

Potassium Iodide is known as Potassium Salt of Hydriodic Acid [HI]. Its Ionic form is K+ I-, and iodide ion being mild reducing agent, it can be easily oxidized to Iodine by powerful oxidizing agent like chlorine. Potassium iodide is often used as a fluorescence quencher. The use of KI to…

Papain is a sulfhydryl protease from Carica papaya latex. (A second protease, chymopapain, and a lysozyme have also been isolated from this same source.) Since native crystalline papain is quite unreactive until acted upon by mild reducing agents such as cysteine or cyanide, it may exist as a…

(H199 Powder) Hanks' salts with L-glutamine Without sodium bicarbonate

Bromocresol purple binds quantitatively with human serum albumin forming a stable complex with absorbance maximum at 600 nm. The intensity of the color produced is directly proportional to the albumin concentration in the sample. Bromocresol Purple is used as a pH indicator (5.2-6.8 : yellow to…

Mineral oil is used as an overlay covering microdrops of culture medium in petri dishes, during the culture of oocytes and embryos in IVF and related procedures. It also has its applications in food processing, cosmetics and pharmaceuticals. A mineral oil is any of various colorless, odorless,…

Pectinase is a macerating enzyme comprised of pectintranseliminase, polygalacturonase, pectinesterase, xylanase, hemicellulases and cellulases. Pectinase is used in plant protoplast preparation to digest cell wall prior to organelle isolation. Pectinase causes random hydrolysis of…

The antibiotics penicillin and streptomycin are used to prevent bacterial contamination of cell cultures due to their effective combined action against gram-positive and gram-negative bacteria. This solution contains 17,000 - 21,000 units/mL of penicillin and 18.0 - 23.0 mg/mL of streptomycin. …

Taurocholic Acid Sodium Salt is the conjugation product of cholic acid with taurine and the principal constituent of the bile of carnivorous animals. Taurocholic Acid Sodium Salt is an anionic detergent used for protein solubilization. Non-sulfated bile salt, physiological transport substrate…

E-64 is an inhibitor for thiol protease, papain, actinidin, ananain, bromelain, calpain and cathepsin B. It is an irreversible cysteine protease inhibitor that has no action on cysteine residues in other plants and does not react with low molecular weight thiols such as 2-mercaptoethanol. Specific…

Lipoprotein- cholesterol is a mixture of lipoprotein cholesterol, phospholipids and fatty acids. For use as a serum-free or serum-reduced media nutrient supplement, calibration of cholesterol values, and enzymatic or non-enzymatic cholesterol determinations.

Preparation Method: Add 5% less distilled water than the desired total volume to a mixing container just large enough to hold the final media volume. Add powdered medium to room temperature (20 -30°C) water with gentle stirring. Do not heat water. Rinse inside of pouch to remove all traces of…

Quinine Hydrochloride is an alkaloid originally isolated from the Cinchona family of South American trees. It is an antimalarial agent, anticholinergic, antihypertensive and hypoglycemic agent. Quinine is an antimalarial agent, anticholinergic, antihypertensive and hypoglycemic agent; also used…

MPure Tissue DNA Extraction Kit is used with the MPure-12 nucleic acid purification system for the extraction and purification of genomic DNA from a variety of animal tissues, swab samples and blood stain. Nucleic acids extracted and purified from MPure Tissue DNA Extraction Kits can be used in a…

Formaldehyde is used as bactericidal agent. Formaldehyde is used as an embalming agent due to its ability to crosslink primary amino groups in proteins through a methylene linkage; however, due to its extremely toxic nature it tends to be avoided. Formaldehyde is also used in numerous industrial…

Dextran is a high molecular weight polymer of anhydroglucose. It is composed of approximately 95% alpha-D-(1-->6) linkages. The remaining a(1-->3) linkages account for the branching of dextran. Dextran has long and hydrophilic spacer arms that improves the performance of immobilized proteins…

Polyethyleneimine is a branched chain polymer having a ratio of 1:2:1 of primary:secondary:tertiary amines with a branching site every 3 to 3.5 nitrogen atoms and a base structure of (CH₂CH₂NH)x. The nitrogen atoms must be protonated to achieve cationic charge, so the cationic…

Analysis Note The total protein is measured using the Biuret procedure with bovine albumin as the standard; F/P ratio is calculated using absorbance at 491.5nm. Antibody titer of the IgG fraction is standardized by equivalence-point precipitation. The IgG fraction is tested for purity and…

Used primarily for lysis of gram positive and gram negative bacteria, fungal tissue and spores, yeasts, molds, filamentous algaes and biofilms. 0.5mm Yttria-Stabilized Zirconium Oxide Spheres High density and hardness Smooth surface minimizes shearing Ideal for cracking very tough…

This human protein is prepared by a modification of the Cohn procedure. Albumin may be used to eliminate background interference in ELISA's or other enzyme assay systems. It is also used in absorption, distribution, metabolism, and excretion (ADME) pharmacological research; cell culture;…

To ensure that the product titer falls within the required range, antibody titer is standardized by microtiter plate ELISA with rabbit IgG. The antibody is tested for purity at 10 mg/mL by immunoelectrophoresis. The antibody is goat IgG; no trace of albumin is detected. Antibody and highly…

Peptone a water-soluble product, is a mixture of polypeptides and amino acids formed by the partial hydrolysis of protein. Peptone is produced from porcine and/or bovine tissues. The tissues are digested enzymatically to create small peptides and then spray dried. Peptone is used in culture…

L-glutamic dehydrogenase is a pyridine nucelotide enzyme which catalyzes the reversible oxidative deamination of L-glutamate to α-keto-glutarate and ammonia. L-glutamic dehydrogenase plays a unique role in mammalian metabolism. The reverse reaction catalyzed by this enzyme is the only pathway…

Roll & Grow Plating Beads are sterile, easy to use, and spread cells in just seconds per plate. Even colony distribution Eliminates hazards of flame and ethanol No need for fragile glass "hockey sticks" Sterile Roll & Grow Plating Beads are provided in two skirted…

Thioglycolic acid protects tryptophan in amino acid analysis,and also mediates formation of ATP from ADP. It lowers the oxidation-reduction potential and neutralises mercurial preservatives. An inhibitor of fatty acid oxidation. An agent that prevents the metabolism of fatty acids and stimulates…

Cetylpyridinium chloride is used is in some types of mouthwashes, toothpastes, lozenges, throat sprays, breath sprays, and nasal sprays. It is an antiseptic that kills bacteria and other microorganisms. It has been shown to be effective in preventing dental plaque and reducing gingivitis.

2-8 CELLsium is a cytoprotective, protein free, ready-to-use bio-solution without Dimethyl Sulfoxide (DMSO) for the short-term storage of cells or tissue. For short-term storage, add cold (2-8 °C) 2-8 CELLsium at a recommended cell concentration of 1 x 10 6 mL to 8 x 10 6 mL. The 2-8 CELLsium…

For use with DOB or YNB with the appropriate nitrogen and carbon sources to custom formulate your own selective Synthetic Defined yeast media. Single dropout of Complete Supplement Mixture (CSM) of amino acids for S. cerevisiae growth media. Time tested formulation is available in complete and…

Goat Serum is a pooled preparation obtained from a normal donor herd. Applications Serum, Goat is suitable replacement for FBS on most cell lines and primary cultures for Biomedical Research - virus isolation and identification, virological studies, etc. It is used as an effective NBCS…

Red dye facilitates phase visualization Improves recovery Ready-Red is a proprietary mixture of chloroform and isoamylalcohol that can be used to remove traces of phenol from the aqueous phase or can be mixed directly with AquaPhenol (void volume in AquaPhenol bottle is adapted to this…

10-100uL Positive Displacement Pipettes Tips (Thomas No. 1177Q83) Replacement tips for for 10-100uL postive displacement pipettor catalog number 07WS1075 pipettor. Capillaries and pistons are pre-assembled and presterilized, in racks of 60. Test Parameters PeroxySafe MSA; PeroxySafe STD;…