What is cell plating in cell culture?
Cell plating in cell culture refers to evenly distributing cells onto a culture dish, flask, or multi-well plate to allow them to attach and grow. This step is critical for creating a uniform cell monolayer, ensuring consistent growth conditions and experiment reproducibility.
What does a 96 well culture plate do?
People use 96-well culture plates in laboratories for high-throughput cell culture and assays. Each can serve as an individual microenvironment, allowing researchers to grow, treat, and analyze cells or perform experiments in parallel. People often use the plates for drug screening, enzyme activity assays, and ELISA (enzyme-linked immunosorbent assay).
Are there different 96 plate well sizes?
The size of each well in a standard 96-well plate can vary slightly depending on the manufacturer. Typically, the diameter of a well is around 6.4 mm, with a depth of approximately 10 to 15 mm, depending on the plate's design. Each well usually holds between 300 µL and 400 µL, although the working volume is often limited to 200 to 250 µL to prevent spillage or evaporation. The overall dimensions of a 96-well plate are standardized at 127.76 mm by 85.48 mm, ensuring compatibility with most laboratory equipment, such as plate readers and robotic systems.