Buffer Solutions

A buffer is a solution created to resist pH changes when introducing internal and external influences. Buffers also maintain intercellular and extracellular pH within the physiological range, providing minimal interference in biological processes. At Thomas Scientific, we have an extensive range of buffers available, including sodium phosphate buffers, blue buffers and LDS sample buffers. We offer a selection of buffers and native gels that carry both positive and negative charges. Buffers are suitable for biochemical research applications such as cell cultivation, isolation of cells, and enzyme assays.

Buffer Solutions

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  • Buffer, pH 7.00, Yellow, Standardized

    Composition: Water 98.73%, Sodium Phosphate Dibasic 0.79%, Potassium Dihydrogen Phosphate 0.47%, Sodium Azide 0.01%, Brilliant Yellow 0% Boiling Point: Approximately 100°C Density: 1 Melting Point: Approximately 0°C Color: Yellow liquid Physical State: Liquid pH Range: 7 Solubility Information:...

  • Orion® pH Buffers

    Thermo Scientific Orion

    Orion pH buffers are manufactured under ISO 9000 quality standards. Buffers are color-coded for ease of use, packaged with buffer/temperature tables for accurate calibrations and NIST traceability. All-in-One Buffer kit (1233M21) includes pint bottles of 4 (pink), 7 (yellow) and 10 (blue) buffers,...

  • pH Electrode Solutions

    Thermo Scientific Orion

    Electrode Storage Solution 475 mL plastic bottle pH of solution is 6.2 to 7.4 For short term storage of all electrodes for one day to a week. Electrode Filling Solution Internal filling solution for Ross-type electrodes (3M KCl). In packages of five 60 mL bottles.

  • Color-Coded pH Buffers

    Standardized and certified traceable to NIST Standard Color-coded for quick reference Accuracy ±0.01 pH at 25°C pH temperature chart, lot numbers and expiration dates on each label pH 4.0 buffer is colored red, 7.0 buffer is yellow or green and 10.0 buffer is blue. Thomas numbers 4120R54,...

  • RPI Sodium Phosphate Dibasic, Anhydrous

    RPI (Research Products International)

    The RPI anhydrous sodium phosphate dibasic is suitable for use in research facilities and chemical laboratories to conduct wide range of experiments. It is available in sturdy bottle that endures heavy and rigorous usage. Application: For Research or Further Manufacturing use only

  • pH Buffer Solutions

    Choose from economical solution bottles or individually sealed pH buffer pouches. NIST traceable materials Labeled with pH vs. temperature tables for accurate calibration All Oakton pH buffer solutions are freshness dated, and standardized against NIST-traceable references to ensure quality....

  • Buffer Standard, pH 1.00

    Meets requirements of both ISO 17025 and ISO 17034 Certified Reference Materials, made under the strictest standards for stability, homogeneity, and accuracy Tested to tolerances of 0.01 pH units Supported by detailed Certificate of Analysis including temperature variation values and stated...

  • pH 4, 7, 10 Buffer Kit

    6 x 500mL kit contains 2 x 500 ml of each pH 4 red (GFS 1634), pH 7 yellow (GFS 1639) and pH 10 blue (GFS 1645). The 3 x 4L kit contains 1 x 4L of each of the above items.

  • Buffer Standard, pH 9.18

    Meets requirements of both ISO 17025 and ISO 17034 Certified Reference Materials, made under the strictest standards for stability, homogeneity, and accuracy Tested to tolerances of 0.01 pH units Supported by detailed Certificate of Analysis including temperature variation values and stated...

  • Buffer Standard, pH 6.86

    Meets requirements of both ISO 17025 and ISO 17034 Certified Reference Materials, made under the strictest standards for stability, homogeneity, and accuracy Tested to tolerances of 0.01 pH units Supported by detailed Certificate of Analysis including temperature variation values and stated...

  • Fluka Sodium hydroxide solution volumetric, 0.5  M NaOH (0.5N)

    Chemical name: Sodium hydroxide Product Brand: Analytical Material form: Solution Density (g/cm3): 1.02 Molecular weight: 40 Molecular formula: HNaO UN Number: UN 1824

  • BD BBL™ Prepared Bottled Media: Buffered Sodium Chloride Peptone Solution (pH 7.0)

    Used to make suspensions of organisms for testing growth promoting and inhibitory properties of media when examining non-sterile pharmaceutical products for specified microorganisms Fluid provides osmotic stability, stable pH value Maintains viability of microorganisms during preparation of samples...

  • Buffer Standard, pH 10.00

    Meets requirements of both ISO 17025 and ISO 17034 Certified Reference Materials, made under the strictest standards for stability, homogeneity, and accuracy Tested to tolerances of 0.01 pH units Supported by detailed Certificate of Analysis including temperature variation values and stated...

  • pH Buffers

    Jenway (Cole-Parmer)

    pH 4 Buffer, Red, 500mL

  • Traceable® pH Buffer Standards (CRM)

    The most accurate pH Standards available anywhere Certified Reference Material Traceable® Certificate supplied Highest accuracy available Traceable® pH Standards, Certified Reference Materials, are 100% compatible with all instruments and probes. Accuracy for the Traceable® Buffers at...

  • DuraCal pH Buffers

    Our complete range of patented buffer solutions provide pH stability of up to five years. High buffering capacity provides rapid and stable calibration.

  • 1M TRIS-HCl, pH 7.5

    Quality Biological

    Tris is compatible with many enzymes in molecular biology, for example DNA modifying enzymes, because its buffering range is between 7.1-8.9. The lower pH 7.4 can be associated more with maintaining RNA, however, the pH 8.0 works equally as well with both DNA and RNA nucleic acids. Applications Tris...

  • pH Electrode Cleaning Kits

    Thermo Scientific Orion

    Extends the life of electrodes Prevents cross-contamination Removes bacterial or protein contaminants All kits contain four 1 oz. bottles of cleaning solution, a 15 mL beaker and a pipette. Sample Kit - includes one bottle each of cleaning solutions A, B, C, D Solution A - for removing protein...

  • High-Accuracy pH Buffer Solutions

    pH Calibration buffers for tight tolerances For meters with 0.001 resolution Buffers are color-coded for easy visual identification.

  • Certipur® Buffer Solution, pH 7.00

    pH: 7.0 (H2O, 20°C) Solubility: (20°C) soluble Melting Point: -5°C Boiling Point: 109°C Density: 1.01 g/cm3 (20°C)

  • Buffer solution pH 10.00

    109409 Buffer solution pH 10.00 (boric acid/potassium chloride/sodium hydroxide), traceable to SRM from NIST and PTB pH 10.00 (25°C) Certipur®

  • NPP (1X) ELISA ALKALINE PHOSPHATASE SUBSTRATE, 100mL, Liquid - clear to pale yellow

    NPP (1X) ELISA ALKALINE PHOSPHATASE SUBSTRATE, 100mL, Liquid - clear to pale yellow, Expiration date is one (1) year from date of opening.

  • 1X RIPA Lysis Buffer 5-PACK (5 x 50 ml), 1Each, Liquid (sterile filtered)

    This product is ready-to-use as a working 1X solution and requires no further dilution. 1X RIPA Lysis Buffer is intended for the extraction of cellular proteins for the efficient lysis of cells and solubilization of protein, while minimizing protein degradation and maintaining protein...

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What is nuclease free water?

Nuclease-free water is highly purified water removed of nucleases. This type of water is essential in molecular biology experiments, such as PCR, RNA sequencing, or DNA extraction, where the integrity of nucleic acids is crucial. By using nuclease-free water, scientists ensure that their samples won't be degraded by unwanted enzymes, preserving the accuracy and reliability of their experiments.

Why is nuclease free water used in PCR?

Nuclease-free water is essential in PCR (Polymerase Chain Reaction) to prevent degradation of the DNA template and primers. Nucleases are enzymes that can degrade DNA or RNA, which would compromise the accuracy and success of the PCR process.