Buffer Solutions

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A buffer is a solution created to resist pH changes when introducing internal and external influences. Buffers also maintain intercellular and extracellular pH within the physiological range, providing minimal interference in biological processes. At Thomas Scientific, we have an extensive range of buffers available, including sodium phosphate buffers, blue buffers and LDS sample buffers. We offer a selection of buffers and native gels that carry both positive and negative charges. Buffers are suitable for biochemical research applications such as cell cultivation, isolation of cells, and enzyme assays.

Buffer Solutions

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  • pH Electrode Solutions

    Thermo Scientific Orion

    Electrode Storage Solution 475 mL plastic bottle pH of solution is 6.2 to 7.4 For short term storage of all electrodes for one day to a week. Electrode Filling Solution Internal filling solution for Ross-type electrodes (3M KCl). In packages of five 60 mL bottles.

  • spex® Certified Buffer Standard

    Certified pH Buffers SPEX CertiPrep pH Buffers are accurate and certified to ISO/IEC 17025:2005 and ISO 17034:2016 requirements. Buffers pH 2 through pH 12 are conveniently packaged in 500 mL bottles. Certified pH buffers are made using high purity starting materials and ASTM Type I Water and are...

  • Buffer, Phosphate, pH 7.2

    For Biochemical Oxygen Demand (BOD) testing Formula: Mixture

  • Color-Coded pH Buffers

    Standardized and certified traceable to NIST Standard Color-coded for quick reference Accuracy ±0.01 pH at 25°C pH temperature chart, lot numbers and expiration dates on each label pH 4.0 buffer is colored red, 7.0 buffer is yellow or green and 10.0 buffer is blue. Thomas numbers 4120R54,...

  • Color-Coded pH Buffer Solutions

    chemForce® (powered by Thomas Scientific)

    Harmonized #: 3822.00.0002|5090 Shelf Life (months): 24 Specific Gravity: 1.00 DOT: Not regulated Physical State: Liquid Flash Point: Not applicable Solubility: Miscible Storage Temperature: Ambient Storage Code: General Traceable to NIST standards

  • VeriSpec® pH Buffer and Conductivity Standard

    Ricca’s VeriSpec® pH Buffer and Conductivity Standard Certified Reference Materials are ISO 17025/17034 accredited to provide the highest level of quality assurance. These products have undergone rigorous evaluations of manufacturing methods, quality control, stability, and homogeneity. As...

  • Orion® pH Buffers

    Thermo Scientific Orion

    Orion pH buffers are manufactured under ISO 9000 quality standards. Buffers are color-coded for ease of use, packaged with buffer/temperature tables for accurate calibrations and NIST traceability. All-in-One Buffer kit (1233M21) includes pint bottles of 4 (pink), 7 (yellow) and 10 (blue) buffers,...

  • pH Buffer Solutions

    Choose from economical solution bottles or individually sealed pH buffer pouches. NIST traceable materials Labeled with pH vs. temperature tables for accurate calibration All Oakton pH buffer solutions are freshness dated, and standardized against NIST-traceable references to ensure quality....

  • pH Buffers

    Various pH buffers for pH probe calibration. Buffer cases are available with a single buffer type or with an assortment of buffers (2 pints of pH 4, 7, and 10 buffers).

  • Colored pH Buffer Standards

    Inorganic Ventures

    Inorganic Ventures' pH Standard is a certified reference material set in a water matrix for stability. This standard is Traceable to NIST and manufactured in accordance with our stringent Quality Assurance guidelines. One year Expiration Date.

  • Buffer Solution, pH 8.00

    pH (@ 25°C) 8.00 +/- 0.02 NIST Traceable

  • 1X PBS (Phosphate Buffered Saline)

    Ready to use, sterile 1X Phosphate-Buffered Saline solution prepared with 18.2 megOhm water, 0.2 micron sterile filtered and steam sterilized in final packaging. Balanced salt solution used in many biomolecular applications. Ultra pure quality makes buffer ideal for use in a variety of cell culture...

  • Traceable® pH Buffer Standards (CRM)

    The most accurate pH Standards available anywhere Certified Reference Material Traceable® Certificate supplied Highest accuracy available Traceable® pH Standards, Certified Reference Materials, are 100% compatible with all instruments and probes. Accuracy for the Traceable® Buffers at...

  • Molecular Biology Grade UltraPure Water

    Rockland Immunochemicals Inc

    Molecular Biology Grade Ultrapure water is suitable for molecular immunology, molecular biology and nucleic acid methodologies, when applicable.

  • 1X PBS, pH 7.2

    Quality Biological

    PBS (phosphate buffered saline) is a balanced salt solution used for a variety biological and cell culture applications, such as washing cells before dissociation, transporting cells or tissue, diluting cells for counting, and preparing reagents. Endotoxin and Mycoplasma tested Applications PBS is...

  • Buffer Solution, pH 6.00

    pH (@ 25°C) 6.00 +/- 0.02 NIST Traceable

  • TAE [50X] (Tris-Acetate-EDTA)

    Features 2M Tris-Acetate, 0.05M EDTA (pH8.3)    

  • Tricine

    MP Biomedicals

    Tricine, a zwitterionic buffer, was first prepared by Good for use as a buffer for chloroplast reactions. It is structurally similar to Tris, but is much less inhibitory at high concentrations. The name tricine comes from tris and glycine from which it was derived. Tricine can be used in...

  • High-Accuracy pH Buffer Solutions

    pH Calibration buffers for tight tolerances For meters with 0.001 resolution Buffers are color-coded for easy visual identification.

  • Certipur® Buffer Solution, pH 7.00

    pH: 7.0 (H2O, 20°C) Solubility: (20°C) soluble Melting Point: -5°C Boiling Point: 109°C Density: 1.01 g/cm3 (20°C)

  • Buffer solution pH 10.00

    109409 Buffer solution pH 10.00 (boric acid/potassium chloride/sodium hydroxide), traceable to SRM from NIST and PTB pH 10.00 (25°C) Certipur®

  • NPP (1X) ELISA ALKALINE PHOSPHATASE SUBSTRATE, 100mL, Liquid - clear to pale yellow

    NPP (1X) ELISA ALKALINE PHOSPHATASE SUBSTRATE, 100mL, Liquid - clear to pale yellow, Expiration date is one (1) year from date of opening.

  • 1X RIPA Lysis Buffer 5-PACK (5 x 50 ml), 1Each, Liquid (sterile filtered)

    This product is ready-to-use as a working 1X solution and requires no further dilution. 1X RIPA Lysis Buffer is intended for the extraction of cellular proteins for the efficient lysis of cells and solubilization of protein, while minimizing protein degradation and maintaining protein...

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What is nuclease free water?

Nuclease-free water is highly purified water removed of nucleases. This type of water is essential in molecular biology experiments, such as PCR, RNA sequencing, or DNA extraction, where the integrity of nucleic acids is crucial. By using nuclease-free water, scientists ensure that their samples won't be degraded by unwanted enzymes, preserving the accuracy and reliability of their experiments.

Why is nuclease free water used in PCR?

Nuclease-free water is essential in PCR (Polymerase Chain Reaction) to prevent degradation of the DNA template and primers. Nucleases are enzymes that can degrade DNA or RNA, which would compromise the accuracy and success of the PCR process.