Buffer Solutions

A buffer is a solution created to resist pH changes when introducing internal and external influences. Buffers also maintain intercellular and extracellular pH within the physiological range, providing minimal interference in biological processes. At Thomas Scientific, we have an extensive range of buffers available, including sodium phosphate buffers, blue buffers and LDS sample buffers. We offer a selection of buffers and native gels that carry both positive and negative charges. Buffers are suitable for biochemical research applications such as cell cultivation, isolation of cells, and enzyme assays.

Buffer Solutions

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  • pH Electrode Solutions

    Thermo Scientific Orion

    Electrode Storage Solution 475 mL plastic bottle pH of solution is 6.2 to 7.4 For short term storage of all electrodes for one day to a week. Electrode Filling Solution Internal filling solution for Ross-type electrodes (3M KCl). In packages of five 60 mL bottles.

  • Clear pH Buffer Solutions

    chemForce® (powered by Thomas Scientific)

    Harmonized #: 2806.10.0000|0000 Shelf Life (months): 24 Specific Gravity: 1.00 DOT: Not regulated Appearance: Colorless Physical State: Liquid Flash Point: Not applicable Solubility: Miscible Storage Temperature: Ambient Storage Code: General

  • Color-Coded pH Buffer Solutions

    chemForce® (powered by Thomas Scientific)

    Harmonized #: 3822.00.0002|5090 Shelf Life (months): 24 Specific Gravity: 1.00 DOT: Not regulated Physical State: Liquid Flash Point: Not applicable Solubility: Miscible Storage Temperature: Ambient Storage Code: General Traceable to NIST standards

  • Color-Coded pH Buffers

    Standardized and certified traceable to NIST Standard Color-coded for quick reference Accuracy ±0.01 pH at 25°C pH temperature chart, lot numbers and expiration dates on each label pH 4.0 buffer is colored red, 7.0 buffer is yellow or green and 10.0 buffer is blue. Thomas numbers 4120R54,...

  • TG Buffer (TRIS-GLYCINE) 10x Premix powder - 1PK(4L)

    The Bio Basic Inc. TG buffer (tris-glycine) 10x premix powder is suitable for laboratory and research use.

  • TG-SDS Buffer (Trisbase-Glycine-SDS) 10X Premix powder - 1PK(4L)

    The Bio Basic Inc. TG-SDS buffer (trisbase-glycine-SDS) 10x premix powder is suitable for laboratory and research use.

  • Buffer, Water Hardness, with Magnesium EDTA

    Formula: Mixture

  • Orion® pH Buffers

    Thermo Scientific Orion

    Orion pH buffers are manufactured under ISO 9000 quality standards. Buffers are color-coded for ease of use, packaged with buffer/temperature tables for accurate calibrations and NIST traceability. All-in-One Buffer kit (1233M21) includes pint bottles of 4 (pink), 7 (yellow) and 10 (blue) buffers,...

  • Buffer Solution, pH 7.38

    pH (@ 25°C) 7.38 +/- 0.02 NIST Traceable

  • 1X PBS, pH 7.4

    Quality Biological

    PBS (phosphate buffered saline) is a balanced salt solution used for a variety biological and cell culture applications, such as washing cells before dissociation, transporting cells or tissue, diluting cells for counting, and preparing reagents. Endotoxin and Mycoplasma tested Applications PBS is...

  • MOPS, Free Acid

    RPI (Research Products International)

    Molecular Biology Grade Heavy Metals:<5 ppm pka: (20°c) 7.20 ± 0.2 ph range: 6.5-7.9<>

  • Potassium Phosphate Monobasic

    Potassium phosphate is a reagent with high buffering capacity. It occurs in several forms: monobasic, dibasic, and tribasic (K3PO4). Most pH neutral potassium phosphate buffer solutions consist of mixtures of the monobasic and dibasic forms to varying degrees, depending on the desired pH. Potassium...

  • ACK Lysing Buffer

    Quality Biological

    Ammonium-Chloride-Potassium (ACK) Lysing Buffer is used for the lysis of red blood cells in samples containing white blood cells, such as EDTA-treated whole blood, buffy coats, and bone marrow. Applications ACK Lysing Buffer is used to lyse red blood cells in samples, such as EDTA-treated whole...

  • Calibration, Maintenance & Cleaning Solutions

    Milwaukee offers a wide range of calibration, maintenance & cleaning solutions. The use of calibration and cleaning solutions is fundamental for the correct use of electrodes and for obtaining the most accurate and reproducible readings. Often readings are not correct because the sensors have not...

  • Traceable® pH Buffer Standards (CRM)

    The most accurate pH Standards available anywhere Certified Reference Material Traceable® Certificate supplied Highest accuracy available Traceable® pH Standards, Certified Reference Materials, are 100% compatible with all instruments and probes. Accuracy for the Traceable® Buffers at...

  • Traceable® ph Buffer Standards (CRM), Color Coded

    labForce® (powered by Thomas Scientific)

    The MOST accurate pH Standards available anywhere now color-coded for convenience Traceable® pH Standards, Certified Reference Materials, are 100% compatible with all instruments and probes. Accuracy for the Traceable® Buffer at 25°C is ±0.010 pH—the most precise available....

  • Optimum Results Filling Solutions

    Thermo Scientific Orion

    For use with ionplus® Sure-Flow® ISE electrodes Little or none of the ion of interest present helps prevent sample contamination Application-specific solution formulation improves response time Small temperature coefficients reduce electrode potential shifts from changing sample...

  • HEPES, Free Acid, Molecular Biology Grade

    HEPES, Free Acid, Molecular Biology Grade | CAS 7365-45-9 | Calbiochem | 25GM

  • MES Monohydrate

    RPI (Research Products International)

    Buffer for use in plant tissue culture. pKa (20°C): 6.15 pH range: 5.5 - 6.7

  • Color-Coded pH Buffer Solution Set

    chemForce® (powered by Thomas Scientific)

    Size: 3 x 500 mL Harmonized #: 3822.00.0002|5090 Shelf Life (months): 24 Specific Gravity: 1.00 DOT: Not regulated Appearance: Color coded Physical State: Liquid pH: 4, 7, 10 Flash Point: Not applicable Solubility: Miscible Storage Temperature: Ambient Storage Code: General

  • HEPES, Sodium Salt

    RPI (Research Products International)

    pKa (20°C): 7.45-7.65 pH Range: 6.8-8.2

  • pH Buffer Powders

    Micro Essential

    Triple protective foil envelope of aluminum, polyethylene and paper which keeps the buffers dry and free flowing Makes a precise buffer solution when dissolved in 500 mL of distilled water Five envelopes per package 

  • Hepes

    MP Biomedicals

    HEPES is a zwitterionic organic chemical buffering agent; one of the twelve Good's buffers. HEPES has been utilized extensively for research and commercial applications in the cell culture and tissue culture industries. HEPES is widely used in cell culture, largely because it is better at...

  • SIGMA Triethylammonium bicarbonate buffer, 1.0 M, pH 8.5&#x00b1;0.1

    Synonyms: Triethylammonium hydrogen carbonate buffer CAS No.: 15715-58-9 Density: 1.02 g/mL at 25 °C(lit.) Application: Buffer for use in ion-exchange chromatography and electrophoresis.

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What is nuclease free water?

Nuclease-free water is highly purified water removed of nucleases. This type of water is essential in molecular biology experiments, such as PCR, RNA sequencing, or DNA extraction, where the integrity of nucleic acids is crucial. By using nuclease-free water, scientists ensure that their samples won't be degraded by unwanted enzymes, preserving the accuracy and reliability of their experiments.

Why is nuclease free water used in PCR?

Nuclease-free water is essential in PCR (Polymerase Chain Reaction) to prevent degradation of the DNA template and primers. Nucleases are enzymes that can degrade DNA or RNA, which would compromise the accuracy and success of the PCR process.