Phosphorylation and dephosphorylation of proteins controls the processes of cell differentiation, proliferation, signal transduction, and apoptosis. Covalent binding of phosphate groups can change the conformation of proteins and the binding capacity of ligands. During protein extraction from animal cells or tissues, phosphorylated proteins are easily degraded by cell bodies and exogenous phosphatases.
Our Phosphatase Inhibitor Cocktail contains a mixture of various phosphatase inhibitors, including tyrosine phosphatase, serine phosphatase, threonine phosphatase, acid, and alkaline phosphatase. It is suitable for protein extraction from animal cells or tissue and can maintain protein phosphorylation to the greatest extent. It is often used in the study of protein kinase activity and signal transduction in animal cells or tissues.
Application Notes:
Dilute 1/10 in RIPA or Lysis Buffer
Usage Statement:
Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use.
Key Applications:
Inhibitor cocktail for the inhibition of phosphatase activity in cell lysis samples
Key features and details
- Inhibits the activity of phosphatases in cell lysate samples
- Helps maintain proteins in their phosphorylated state
Application Notes: Dilute 1/10 in RIPA or Lysis Buffer
Pack Size: 10 mL
Personal Protective Equipment: Nitrile/Latex Gloves
Preparation Method: Dilute as the user manual describes
Storage : 4 °C
Usage Statement: Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.