G-Biosciences Chemicals Enzymes and Inhibitors
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LongLife™ DNase
G-BiosciencesEnzymes regularly used in laboratory applications often require preparation of fresh solution before each use. Making fresh enzyme solution for each application is time consuming and wasteful. A wide variety of enzyme preparations in a ready-to-use format are offered. LongLife™ enzyme…
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Proteinase K
G-BiosciencesProteinase K (also protease K, endopeptidase K, peptidase K or Tritirachiumalkaline phosphatase) (EC 3.4.21.64) is a non-specifc, broad spectrum serine protease that is isolated from the saprophytic fungus Tritirachium album. Proteinase K is routinely used for the purification of target…
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Immobilized Trypsin
G-BiosciencesImmobilized Trypsin is TPCK Treated Trypsin immobilized on 4% agarose that eliminates the contamination of protein digests by the trypsin. The immobilized trypsin is readily removed by separating the agarose from the digestion solution. Trypsin is a serine endopeptidase that specifically…
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Staurosporine
G-BiosciencesStaurosporine is a strong inhibitor of phosphalipid/Ca2+ dependent protein kinase and platelet aggregation. It is most commonly used as an inducer of apoptosis in several mammalian cell types. It is also used to study role of protein kinases in signal transduction as it inhibits other protein…
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Taq DNA Polymerase
G-BiosciencesTaq DNA Polymerase is a highly thermostable recombinant DNA polymerase derived from the thermophile, Thermus aquaticus. The molecular weight of the recombinant protein is 94kD.. The Taq polymerase is able to amplify DNA up to 5kb with an elongation velocity of 0.9-1.2kb/min at 70-75°C. The…
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Trypsin
G-BiosciencesTrypsin is a serine endopeptidase that specifically cleaves peptide bonds on the carboxy side of s-aminoethyl cysteine, arginine and lysine residues. Typically there is little or no cleavage at arginyl-proline and lysyl-proline bonds. This trypsin is purified from bovine pancreas, 1X…
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Pfu Polymerase
G-BiosciencesPfu DNA polymerase, derived from the hyperthermophilic archae Pyrococcus furiosus, has superior thermostability and proofreading properties compared to other thermostable polymerase. Its molecular weight is 90 kD. It can amplify DNA target up to 2kb. The elongation velocity is 0.2~0.4kb/min…
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ProteaseArrest™ for Plant
G-BiosciencesA broad range, 100X concentrated, ready-to-use protease inhibitor cocktail. Plant ProteaseArrest™ inhibits plant serine, cysteine and other plant specific proteases including aminopeptidases, aspartic and metalloproteases. ProteaseArrest Family Our ProteaseArrest™ is a…
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ProteaseArrest™ for Yeast/ Fungal
G-BiosciencesA broad range, 100X concentrated, ready-to-use protease inhibitor cocktail. Yeast/ Fungal ProteaseArrest™ inhibits yeast and fungal serine, cysteine and metalloproteases. ProteaseArrest Family Our ProteaseArrest™ is a highly efficient, liquid format general protease…
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Immobilized Pepsin
G-BiosciencesPepsin is a proteolytic enzyme that is routinely used for the generation of F(ab)2 fragments from immunoglobulin G (IgG). The pepsin has the ability to cleave the heavy chains near the hinge region. One or more of the disulfide bonds that join the heavy chains in the hinge region are…
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TCM Protease Arrest™
G-BiosciencesFor use in tissue culture media A broad range, 200X concentrated, ready-to-use protease inhibitor cocktail sterile filtered for tissue culture media. TCM ProteaseArrest™ inhibits a wide range of serine, cysteine and other specific proteases including aminopeptidasestrypsin-like and acid…
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Trypsin (Porcine), Mass Spectrometry Grade
G-BiosciencesA Chemically Modified, TPCK treated, Affinity Purified Trypsin Trypsin is a serine endopeptidase that specifically cleaves peptide bonds on the carboxy side of s-aminoethyl cysteine, arginine and lysine residues, and typically, there is little to no cleavage at arginyl-proline and lysyl-proline…
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Apoptotic Inducer Set
G-BiosciencesReady-to-use high quality apoptosis inducers allow users an easy tool for induction of apoptosis in cultured cells. These stabilized reagent solutions have a long storage life and are easy to use. The selection of apoptosis inducers include actinomycin D, camptothecin, cycloheximide, dexamethasone,…
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LongLife™ Lysozyme
G-BiosciencesEnzymes regularly used in laboratory applications often require preparation of fresh solution before each use. Making fresh enzyme solution for each application is time consuming and wasteful. A wide variety of enzyme preparations in a ready-to-use format are offered. LongLife™ enzyme…
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Immobilized Pepstatin
G-BiosciencesG-Biosciences Immobilized Pepstatin is for the purification of cathepsins, pepsin, bacterial aspartic proteases, HIV proteases and other molecules that bind pepstatin. Pepstatin is isovaleryl-Val-Val-AHMHA-Ala-AHMHA where AHMHA= (3S, 4S)-4-amino-3-hydroxy-6-methyl-heptanoic acid and is a…
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Recom ProteaseArrest™
G-BiosciencesA broad range, bacterial, 100X concentrated, ready-to-use protease inhibitor cocktail. Recom ProteaseArrest™ offers greater protection for recombinant proteins expressed and purified from bacteria. Inhibits bacterial serine, cysteine, metallo-and other bacterial specific proteases including…
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Adenine Deaminase (EC 3.5.4.2) [2µM]
G-BiosciencesAdenine Deaminase (EC 3.5.4.2) converts Adenine to Hypoxanthine. A highly purified recombinant enzyme protein supplied at a 2µM concentration.
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Luciferase (Firefly, Recombinant)
G-BiosciencesFirefly luciferase is a unique photoprotein used in bioluminescence for high sensitive detection and quantification of ATP and as a reporter for studying gene function and regulation.Luciferase (Firefly, Recombinant) is recombinant 62 kDa protein expressed in E. Coli. Click here to request…
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PhosphataseArrest™
G-BiosciencesThe PhosphataseArrest™ phosphatase inhibitor cocktails are 100X concentrated, ready-to-use solutions that are simply added to your extraction buffers or samples. PhosphataseArrest™ is ideal for inhibition in tissue extractions and cell lysis experiments and is compatible with most…
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Protease Inhibitor Set
G-BiosciencesContains 12 ready-to-use individual protease inhibitors for characterization of protease activity. Each set contains the following protease inhibitors. See individual inhibitors for their specificities and other information.: AEBSF ALLN Antipain, dihydrochloride Aprotinin …
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ProteCEASE™
G-BiosciencesProteCEASE™ is a dry format version of our ProteaseARREST™ for large scale preparative applications and for those who prefer reconstitution prior to use. ProteCEASE™ is a superior general protease inhibitor cocktail that is suitable for purification from mammalian, plant,…
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LongLife™ Zymolyase®
G-BiosciencesEnzymes regularly used in laboratory applications often require preparation of fresh solution before each use. Making fresh enzyme solution for each application is time consuming and wasteful. G-Biosciences offersa wide variety of fresh, ready-to-use enzyme preparations. Our high performance…
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ProteaseArrest™
G-BiosciencesProteaseArrest™ is a general protease inhibitor cocktail solution that is provided as a 100X concentrated, ready-to-use solution. The ProteaseArrest™ 100X solution format is suitable for small, analytical sample applications, as >95% inhibition is achieved by adding 10µl…
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FOCUS™ ProteaseArrest™
G-BiosciencesFOCUS™ ProteaseArrest™ is a ready-to-use, 100X concentrated, broad range protease inhibitor cocktail that is fully compatible with 2D electrophoresis and subsequent mass spectrometry. The protease inhibitor cocktail contains reversible and irreversible inhibitors of serine, cysteine,…
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Aprotinin
G-BiosciencesAlso known as bovine pancreatic trypsin inhibitor. Activity: Approx 6,000 KIU/mg Specificity: A broad range, competitive and reversible inhibitor of chymotrypsin, plasmin, trypsin, kallikrein and other serine proteases Solubility: Soluble in water (Stock solution: 10mM) A…
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Cycloheximide [100mM]
G-Biosciences(4-{(2R)-2-[(1S,3S,5S)-3,5-dimethyl-2-oxocyclohexyl]-2-hydroxyethyl}piperidine-2,6-dione) An inhibitor of protein synthesis by interfering with the translocation step (movement of two tRNA molecules and mRNA on ribosome) in protein synthesis, thus blocking translational elongation. …
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SG-Glutamic-C™
G-BiosciencesSG -Glutamic-C ™ is a serine endopeptidase, from S. aureus V8 , that is highly specific for the cleavage of peptide bonds at the carboxy side of either aspartic or glutamic acid, depending on the buffer used. In Tris-HCl buffer, in particular in the absence of phosphate ions, the enzyme is…
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Recombinant Human Caspases
G-BiosciencesRecombinant human caspases 1-3 and 6-10 are supplied lyophilized in quantities of 25 units. One unit of the specific recombinant caspase is the enzyme activity that cleaves 1nmol of a specific caspase substrate per hour at 37ºC in a reaction solution containing 50mM HEPES (pH7.2), 50mM…
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Immobilized Papain
G-BiosciencesPapain is a cysteine protease enzyme (EC 3.4.22.2) that has the endopeptidase activity to cleave immunoglobulin G molecules in the hinge region. This cleavage results in the generation of three ~50kDa fragments; two Fab domains and a Fc domain. The papain-digested antibody is unable to promote…
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Taq PLUS DNA Polymerase Mastermix
G-BiosciencesTaq Plus Mix [2X] is a premixed, ready-to-use solution containing Taq Plus DNA Polymerase , dNTPs , Mg2+ and Reaction Buffer at optimal concentrations for efficient amplification of DNA templates by PCR. To prepare the final PCR, only primers and template DNA are added. Plus Mix contributes to…
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4-(2-Aminoethyl) benzenesulfonyl fluoride hydrochloride Specificity: Specific irreversible inhibitor of serine proteases, including chymotrypsin, kallikrein, plasmin, thrombin and trypsin. A stable non-toxic alternative to PMSF. Solubility: Soluble in water Features …
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Immobilized Aprotinin
G-BiosciencesAprotinin is a monomeric globular protein which inhibits several serine proteases including trypsin, chymotrypsin, plasmin and kallikrein. Furthermore, its action on kallikrein leads to inhibition of formation of factorXIIa. The binding of aprotinin to proteases is reversible with dissociation of…