Catalase from bovine liver is a homotetrameric enzyme that is primarily located in peroxisomes. Activates the decomposition of hydrogen peroxide Natural antioxidant used to study roles of reactive oxygen species Catalyzes the decomposition of hydrogen peroxide into water and oxygen …

Alcohol oxidase has been detected in several strains of yeast, such as Candida, Pichia, and Hansenula, that utilize methanol as a sole carbon and energy source. Some of these enzymes have been isolated and partially characterized. Alcohol oxidase plays a major role in the metabolism of methanol…

Choline oxidase (EC 1.1.3.17) is an enzyme that catalyzes the chemical reaction Choline + O2 betaine aldehyde + H2O2. Thus, the two substrates of this enzyme are choline and O2, whereas its two products are betaine aldehyde and H2O2. This enzyme belongs to the family of oxidoreductases,…

Hyaluronidase is a glycoprotein containing 5% mannose and 2.17% glucosamine, it catalyzes the random hydrolysis of 1,4-linkages between 2-acetamido- 2-deoxy- β-D-glucose and D-glucose residues in hyaluronate. Hyaluronidase from bovine testes is a tetramer consisting of 4 equal subunits with a…

Hyaluronidase is a glycoprotein containing 5% mannose and 2.17% glucosamine, it catalyzes the random hydrolysis of 1,4-linkages between 2-acetamido- 2-deoxy- b-D-glucose and D-glucose residues in hyaluronate. Hyaluronidase from bovine testes is a tetramer consisting of 4 equal subunits with a…

Alkaline phosphatase is a hydrolase enzyme responsible for removing phosphate groups from many types of molecules, including nucleotides, proteins, and alkaloids. It is most effective in an alkaline environment. It is sometimes used synonymously as basic phosphatase. Alkaline phosphatase is used…

Collagenases degrade native helical collagen fibrils. Plays an important role in connective tissue metabolism Produced by specific cells involved in repairs and remodeling processes Type II enzyme that contains greater clostripain activity Collagenase is used for collagen…

Aldehyde dehydrogenase belongs to the family of oxidoreductases, specifically those acting on the aldehyde or oxo group of donor with NAD+ or NADP+ as acceptor. Aldehyde dehydrogenase oxidizes a number of aliphatic and aromatic aldehydes. Acetyl-GSH is not hydrolyzed. Aldehyde…

Trypsin consists of a single chain polypeptide of 223 amino acid residues. Member of the serine protease family Dissolves blood clots in its microbial form Treats inflammation in its pancreatic form Trypsin cleaves peptides on the C-terminal side of lysine and arginine residues.…

α-Thrombin is a trypsin-like serine protease involved in a multitude of processes in the human body. Thrombin generation is the result of limited proteolysis of the vitamin K-dependent zymogen prothrombin. Thrombin is the last enzyme in the clotting cascade functioning to cleave fibrinogen to…

Dipase is an extremely stable microbial metalloprotease suitable for use in cell cultures. Dispase is suitable for tissue disaggregation and subcultivation procedures since it does not damage cell membranes. Dissolve the lyophilized enzyme in sterile water to 5 mg/mL. Further dilution with…

Pepsin, an acid protease, contains a proteolytic enzyme. Pepsin contains the "cathepsin" component which has milk curdling activity. It has a broad range of substrate activity and demonstrates an esterase acitivity. It generally attacks peptide bonds. Pepsin is a peptidase used to…

Cholesterol Esterase is useful for enzymatic determination of total cholesterol when coupled with cholesterol oxidase in clinical analysis. Stored intracellularly as cholesterol esters Reversible enzyme Hydrolyzes and synthesizes fatty acid esters of cholesterol and other sterols …

Elastase is an enzyme from the class of proteases (peptidases) that break down proteins. It hydrolyzes peptide bonds, especially those adjacent to neutral amino acids. Hydrolyses elastin Digests hemoglobin, casein, and fibrin One unit will solublize 1 mg of elastin in 20 minutes at…

Papain is a sulfhydryl protease from Carica papaya latex. Cleaves peptide bonds of basic amino acids, leucine, or glycine Hydrolyzes esters and amides One unit will hydrolyze 1.0 µmole of N-alpha-benzoyl-L-arginine ethyl ester (BAEE) per minute at 25°C and pH 6.2 Papain…

β-Galactosidase is useful for the structural investigation of carbohydrate, the determination of lactose (foodstuff analysis) and as an enzyme label for enzyme immunoassay. β-Galactosidase cleaves terminal galactose residues, which are β -1-->14 linked to a monosaccharide,…

Yeast lytic enzyme is used to digest the cell wall of active yeast cells. Yeast lytic enzyme is an enzyme preparation from a submerged culture of Arthrobacter luteus which effectively lyses cell walls of viable yeast cell. It is a lyophilized enzyme partially purified by affinity chromatography.…

Deoxyribonuclease from beef pancreas, DNase I, was first crystallized by Kunitz. It is an endonuclease which splits phosphodiester linkages, preferentially adjacent to a pyrimidine nucleotide yielding 5'-phosphate terminated polynucleotides with a free hydroxyl group on position 3'. The…

Iodoacetamide is a thiol reagent; alkylating reagent for cysteine and histidine residues in proteins. In the alkylation reaction, it reacts with histidine (such as in RNase), methionine and sulfhydryl groups of many proteins. Iodoacetamide can react with low molecular weight thiol compounds such as…

Pepsin, an acid protease, contains a proteolytic enzyme. Pepsin contains the "cathepsin" component which has milk curdling activity. It has a broad range of substrate activity and demonstrates an esterase acitivity. It generally attacks peptide bonds. Pepsin is a peptidase used to…

Pectinase is a macerating enzyme comprised of pectintranseliminase, polygalacturonase, pectinesterase, xylanase, hemicellulases and cellulases. Pectinase is used in plant protoplast preparation to digest cell wall prior to organelle isolation. Pectinase causes random hydrolysis of…

β-Glucosidase is a glucosidase enzyme. It is an exocellulase with specificity for a variety of β-D-glycoside substrates. β-Glucosidase is used in the synthesis of glucosides and fucosides with various potential applications in pharmaceutical, cosmetic and detergent industries,…

MAO inhibitor; inhibits platelet aggregation.

Thiamine (Vitamin B1) is one of the essential vitamins and is required for carbohydrate metabolism. Thiamine hydrochloride is used as a food-additive to add a brothy/meaty flavor to gravies or soups. It is also used as food supplement. Used in fluorometric determination of mercury.

Catalase, Fungal suspension from from Aspergillus niger is long-acting, extremely stable form of catalase that is active over a wide pH range. Composed of four protein subunits Each subunit contains a heme group bound to its active site Catalase is used for the removal of peroxides,…

Lactase is an enzyme preparation produced by Aspergillus oryzae fermentation. Hydrolyzes Lactose Produces β-D-Galactose and a-D-Glucose One unit will hydrolyze 1.0 µmole of o-nitrophenyl-beta-D-galactoside to o-nitrophenol per minute at pH 4.5 and 30°C …

Acetyl-CoA is produced via beta-oxidation of fatty acids, via the metabolism of carbohydrates - glucose 6-phosphate to pyruvate to acetyl-CoA and via the catabolism of amino acids. Acetyl-CoA has a number of metabolic opportunities. It is metabolized in the tricarboxylic acid cycle to produce…

This enzyme is useful for enzymatic determination of creatinine when coupled with creatine amidinohydrolase, sarcosine dehydrogenase or sarcosine oxidase and formaldehyde dehydrogenase in clinical analysis. Creatininase from Pseudomonas sp. is a homohexameric enzyme with a molecular mass of…

Ribonuclease belongs to pancreatic ribonuclease family. RNase A is an endoribonuclease that attacks at the 3¢ phosphate of a pyrimidine nucleotide. The sequence of pG-pG-pC-pA-pG will be cleaved to give pG-pG-pCp and A-pG. Ribonuclease A is used to remove RNA from DNA plasmid preparations…

Biliverdin Dihydrochloride is a bile pigment component. An oxidized metabolite of bilirubin caused when bilirubin reacts with reactive oxygen species. Biliverdin IX is composed of alpha, beta, gamma and delta isomers. Biliverdin dihydrochloride was found responsible for cytoprotection. It was…

Glucose oxidase is an FAD-containing glycoprotein. The enzyme is specific for β-D-glucose. O can be replaced by hydrogen acceptors such as 2,6-dichlorophenol indophenol. Glucose oxidase from Aspergillus niger is a dimer consisting of 2 equal subunits with a molecular mass of 80 kDa each. Each…

Ethylenediamine Tetraacetic Acid is a polyamino carboxylic acid hexadentate ligand and a chelating agent. Ethylenediamine Tetraacetic Acid is a chelator of divalent cations. It also inhibits enzymes, such as metalloproteases, that require divalent cations for activity. Zinc-dependent…