Dispase

Dipase is an extremely stable microbial metalloprotease suitable for use in cell cultures. Dispase is suitable for tissue disaggregation and subcultivation procedures since it does not damage cell membranes. Dissolve the lyophilized enzyme in sterile water to 5 mg/mL. Further dilution with…

Synonym(s): Protease from Bacillus polymyxa MDL Number: MFCD02253017 Activity: ≥0.5 units/mg solid Storage: 2-8°C

Synonym(s): Protease from Bacillus polymyxa MDL Number: MFCD02253017 Activity: ≥10 unit/mg solid Storage: 2-8°C

… EDTA, EGTA, Hg 2+ , other heavy metals. Dispase is not inhibited by serum. Working concentration: 0.6 to 2.4 U/ml Working solution: Preparation of stock and working solutions: To produce a 10 mg/ml stock solution, dissolve the lyophilized Dispase ® II enzyme in HEPES-buffered saline…

…should be frozen in aliquots. Avoid repeated freezing and thawing. The working solution diluted with PBS is stable at 2 to 8 °C for 3 days. Specificity: Dispase ® is a non-specific metalloprotease. Legal Information: Dispase is a registered trademark of Godo Shusei Co., Ltd.

…analysis). • Obtain higher experimental reproducibility due to higher lot-to-lot consistency. Application: Liberase ™ DH (Dispase High) Research Grade is used for the dissociation of a broad range of tissue types, where high purity of the enzyme blend is necessary for high…

…concentration: Approximately 1mg/mL (Collagenase: 0.1U/mL, Dispase: 0.8U/mL) Enzyme activity: Collagenase: 0.1 Wünsch U/mg lyophilizate (+25°C, 4-phenyl-azobenzyl-oxycar bonyl-Pro-Leu-Gly-Pro-D-A rg as the substrate) Dispase ® : 0.8 Wünsch U/mg lyophilizate (+37°C, casein…

…culture and passage of human embryonic and induced pluripotent stem cells.Dispase-II enzyme is produced in Bacillus polymyxa. Synonyms: Dispase, Dispase 2 Application: Research Sub CategoryEnzymes & Biochemistry Legal Information: PLURISTEM is a registered trademark of Merck KGaA,…

…from Clostridium histolyticum and Bacillus polymyxa cultures devoid of animal containing reagents. This product is manufactured in an ISO9001:2008 certified enviroment. Synonyms: MMP, Matrix metalloprotease, Dispase, Neutral Protease Application: Research Sub CategoryEnzymes & Biochemistry

…from Clostridium histolyticum and Bacillus polymyxa cultures devoid of animal containing reagents. This product is manufactured in an ISO9001:2008 certified enviroment. Synonyms: MMP, Matrix metalloprotease, Dispase, Neutral Protease Application: Research Sub CategoryEnzymes & Biochemistry

Neutral protease (Dispase®) is designed for primary cell isolation and tissue dissociation. It is commonly used to separate skin epidermis from dermis leaving intact epithelial sheets and stem cell, hepatocyte and other cell isolation applications. However, due to the diversity of the variables…

…enabling the recovery of precious samples and time. EZ-LiFT does not suffer from the drawbacks of other enzyme-based dissociation reagents such as Dispase, Collagenase IV and Accutase. The disadvantages of enzyme-based reagents are that they still require manual removal of differentiated cells,…