Alkaline phosphatase is a hydrolase enzyme responsible for removing phosphate groups from many types of molecules, including nucleotides, proteins, and alkaloids. It is most effective in an alkaline environment. It is sometimes used synonymously as basic phosphatase.
Alkaline phosphatase is used for conjugation to antibodies and other proteins for ELISA, Western blotting, and histochemical detection. It may be used for protein labeling when high sensitivity is required.
Alkaline phosphatase, from Escherichia coli, is a dimeric, non-glycosylated protein which mainly reside in the periplasmic space. Three known isoforms exist. The enzyme requires zinc, and is activated by magnesium. E. coli akaline phosphatase has a broad specificity for phosphate esters.
Specific Activity: ≥30 u/mg protein , Activity- ~1000 u/mL
Key Applications: Conjugation, Protein Labelling
Application Areas: Cell Biology & Analysis; Molecular biology
Research Areas: Cell biology
Product Type: Proteins, Enzymes & Peptides
Presentation: Suspension
Format: Ammonium Sulfate Suspension
Storage & Handling: Store at +4°C, do not freeze.