Triton® X-100 is a alkylaryl polyether alcohol. It is a nonionic surfactant prepared by the reaction of octylphenol with ethylene oxide. The number ("-100") relates only indirectly to the number of ethylene oxide units in the structure. Triton X-100 is a non-ionic surfactant and…

Polyethyleneimine is a branched chain polymer having a ratio of 1:2:1 of primary:secondary:tertiary amines with a branching site every 3 to 3.5 nitrogen atoms and a base structure of (CH₂CH₂NH)x. The nitrogen atoms must be protonated to achieve cationic charge, so the cationic…

1× Park and Terasaki's Modification Of McCoy 5A is used for the primary culture of cells from a wide range of tissues. Recommend for production of virus in primary cell cultures. It is excellent for the propagation of leukocytes, biopsy tissues, a broad range of human and rat normal and…

MP Biomedicals offers a 1000X sterile filtered solution for use with serum-free media. It has the same composition as Cleveland’s Trace Element I. To be added aseptically to media as a supplement. Prior to use of media, it may be necessary to adjust pH and sterile filter (optional, reduces contamination).

Trichloroacetic acid is an acetic acid analogue commonly used to precipitate proteins, DNA and RNA. In the presence of SDS Trichloroacetic acid will precipitate proteins which can then be quantified by the Lowry method. The compound has also found uses as a decalcifier and fixative in microscopy,…

Mineral oil is used as an overlay covering microdrops of culture medium in petri dishes, during the culture of oocytes and embryos in IVF and related procedures. It also has its applications in food processing, cosmetics and pharmaceuticals. A mineral oil is any of various colorless, odorless,…

Isoamy alcohol is routinely used in molecular biology, especially in the purification of DNA. It is widely used in conjunction with phenol and chloroform (25:24:1 phenol : chloroform : isoamyl alcohol) for the removal of proteins from the nucleic acid solutions by extraction. Isoamyl alcohol is…

Human pooled serum is used as a media component to neutralize cell free virus. It's used as a reagent in liposome binding assay and in preparation of sperm. This serum is obtained from pooled, normal clotted human blood.

Dimethyl Sulfoxide is a dipolar, aprotic solvent. It has been shown to accelerate strand renaturation (1-10% concentration) and is believed to give the nucleic acid thermal stability against depurination. Dimethyl Sulfoxide is used to enhance dermal absorption of many chemicals, as a solvent for…

Isopropyl alcohol is widely used in chemistry, biochemistry and molecular biology. Industrial applications include its use in antifreezes, quick-drying oils, and quick-drying inks, and as a solvent for gums, shellac, and essential oils. Isopropyl alcohol has been utilized as a solvent in the…

Hydrogen peroxide is used as a reagent, disinfectant, antiseptic, and propellant. In synthetic chemistry, H2O2 is used for epoxidation and hydroxylation transformations, and is used as the raw oxidant source for generating more complicated and specific organic/inorganic peroxides.

Eosin Y, a red xanthene dye, has been noted to reduce the affinity of Mg2+- ATPase for ATP and increase the enzyme affinity for Mg2+ at concentrations of 10-50 μM. At higher concentrations near 100 μM the affinity for Mg2+- ATPase for the ion-activator has been shown to be reduced. This…

Ammonium acetate is a chemical compound which is derived from the reaction of ammonia and acetic acid. Ammonium acetate is volatile at low pressures. Because of which it has been used to replace cell buffers with non-volatile salts, in preparing samples for mass spectrometry. It is used as a buffer…

Trypan Blue is a blue acid dye with a strong affinity for cellulose containing substrates such as cotton; less affinity for proteinaceous materials. Trypan blue solution may be used in trypan blue based cytotoxitiy and proliferation assays.

Hexane is used in laboratory to extract oil and grease contaminants from water and soil for analysis.

Stabilized Chromogen Solution - This alkaline phosphatase chromogen solution contains the two components in a stabilized solution which may be used as the final step in color development. The resulting product is a permanent, deep-purple stain. It enhances results in both ELISA and immunohistology…

SDS is an anionic detergent used to solubilize and denature protein for Protein Electrophoresis & Blotting. SDS has also been used in large-scale phenol extraction of RNA to promote the dissociation of protein from nucleic acids when extracting from biological material. Surfactant used for…

This serum is collected from Type AB human blood.

Rabbit serum is a component of blood obtained from a rabbit that contains blood plasma without fibrinogen or other clotting factors. Rabbit Serum Combine with BSA to use as a blocking serum in immunocytochemistry and immunohistochemistry. In immunohistochemistry, antibodies are used to detect…

MP Biomedicals offers a 1000X sterile filtered solution for use with serum-free media. It includes selenium, copper, zinc, and iron, as well as ferric citrate for greater stability.

Pluronic® F-68 is a non-ionic surfactant in cell culture used to control shear forces in suspension cultures against possible damage during freezing and thawing, transfer and stirring. It can also be used to reduce foaming in stirred cultures and reduce cell attachment to glass. …

A buffer solution of HEPES can be prepared by any of several methods. The free acid can be added to water, then titrated with approximately one-half mole equivalent of sodium hydroxide or potassium hydroxide to the desired pH. A simple mixing table for preparing 0.05 M HEPES/NaOH has been…

TRITON X-114 is used in biochemical applications to solubilize and separate proteins due to its low cloud point (23 °C). At temperatures above the cloud point, detergent solutions separate into aqueous and detergent-enriched phases. This makes Triton X-114 particularly useful in…

Hexadecane (n-Hexadecane) is an aliphatic long-chain hydrocarbon. It is a reference fuel with the cetane number 100 and is employed in determining the cetane number of diesel fuels. The mechanism of the oxidation of hexadecane in the gas-phase has been described. The density, speed of sound,…

Tweens® are a series of nonionic surfactants derived from sorbitan esters. They are soluble or dispersible in water but differ widely in organic and oil solubilities. Used as oil-in-water emulsifiers in pharmaceuticals, cosmetics, cleaning compounds, etc. Tween® 80 has been widely used…

2-Merceptoethanol is used to reduce disulfide bonds and can act as a biological antioxidant by scavenging hydroxyl radicals. 2-Merceptoethanol is used to reduce disulfide linkages in solubilizing proteins for gel electrophoresis. 2-mercaptoethanol is readily oxidized in air to a disulfide,…

EDTA solution for use as a gentle non-enzymatic cell dissociation reagent.

TMB is a chromogenic substrate for Horse Radish Peroxidase (HRP). MP Biolomedicals' TMB Solution is a convenient, non toxic and ready to use stable chromogen solution suitable for peroxidase and pseudo-peroxidase reactions in ELISA, it provides consistent performance and sensitivity to deliver…

To rehydrate the powdered medium, dissolve 29.5 grams in one liter of water. Dispense as desired. Sterilize in the autoclave for 15 minutes at 15 pounds pressure at 121°C (Catalog number 16821 is a pre-sterilized solution). For more rapid growth of pathogenic bacteria, dissolve 1 gram of agar…

Sodium pyruvate is used by cells as an easily accessible carbohydrate source. Additionally, it is involved with amino acid metabolism and initiates the Kreb′s cycle. The 100 mM solution should be diluted 1:100 for most cell culture. The use of sodium pyruvate in Wallen fermentation medium to…

2-Mercaptoethanol is a hybrid of ethylene glycol and 1,2-ethanedithiol. It is used to reduce disulfide bonds and can act as a biological antioxidant by scavenging hydroxyl radicals. It is widely used because the hydroxyl group confers solubility in water and lowers the volatility. Due to its…

1,1,1,3,3,3-Hexafluoro-2-propanol is polar and exhibits strong hydrogen bonding properties enabling it to dissolve substances that serve as hydrogen-bond acceptors. 1,1,1,3,3,3-Hexafluoro-2-propanol is a polar solvent of high ionizing power and facilitates Friedel–Crafts-type reactions, using…