Sodium pyruvate is used by cells as an easily accessible carbohydrate source. Additionally, it is involved with amino acid metabolism and initiates the Kreb′s cycle. The 100 mM solution should be diluted 1:100 for most cell culture. The use of sodium pyruvate in Wallen fermentation medium to enhance the conversion of oleic acid to 10-ketostearic acid by Bacillus sphaericus has been described. A protocol that uses sodium pyruvate to establish stably transfected human B cell lines has been published. It improves coliform recovery when present in culture medium.
In muscle, pyruvic acid (derived from glycogen) is reduced to lactic acid during exertion, which is reoxidized and partially retransformed to glycogen during rest. Involved in a metabolic regulatory pathway activated by mitochondrial oxidants. Pyruvate is involved in respiratory regulation in plants by interacting with alternative oxidase at a conserved cysteine residue. May help prevent hydrogen peroxide mediated cell death. Pyruvate, the anion of pyruvic acid, is the end product of the glycolysis pathway, whereby glucose is converted to pyruvate with the production of ATP.
Concentration: 100 mM
Suitability: Suitable for cell culture
Key Applications: Improves coliform recovery when present in culture medium
Application Areas: Primary Cell Culture
Product Type: Reagents
Density: ~1.267 g/mL (Lit.)
Presentation: Clear, Slightly Yellow Solution
pH: 6.8 (Lit.)
NOTES: Osmolality: 430 - 500 mOsm/kg H2O
Storage & Handling: Store at +4°C