Dimethyl Sulfoxide is a dipolar, aprotic solvent. It has been shown to accelerate strand renaturation (1-10% concentration) and is believed to give the nucleic acid thermal stability against depurination.
Dimethyl Sulfoxide is used to enhance dermal absorption of many chemicals, as a solvent for many organic and inorganic compounds including fats, carbohydrates, dyes, resins, and polymers, in antifreeze or hydraulic fluids, as a cryopreservative for cell cultures, oxidation of thiols and disulfides to sulfonic acids, as a PCR cosolvent to help improve yields, especially in long PCR. DMSO is routinely used in polymerase chan reaction (PCR), amplification of cDNA libraries, DNA sequencing, column-loading buffers for poly(A)+ RNA selection, buffers for the transformation of competent E. coli, and transfection protocols.
Grade: Molecular biology reagent
Assay: ≥99.9%
Key Applications: PCR Cosolvent
Application Areas: Molecular Biology
Product Type: Solvents
Chemical Class: Polar Aprotic
Density: 1.104 g/cm3 at 20°C (Lit.)
Auto Ignition: 573°F (Lit.)
Boiling Point: 189°C at 1013 hPa (Lit.)
Melting Point: 16 -19°C(lit.)
Vapor Density: 2.7 (vs air) (Lit.)
Vapor Pressure: 0.42 mmHg (20°C) (Lit.)
Refractive Index: n20D 1.479 (lit.)
Presentation: Liquid
Format: Liquid
Dielectric Constant: 45 (Lit.)
Water Content: ≤0.1%
NOTES: Residue after Evaporation: ≤0.01%, Titratable Acid: ≤0.001meq/g; Autoprotolysis constant ~33 at 25°C
Solubility: Soluble in water, methanol, acetone, ether, benzene, chloroform.
Sterilization of Solutions: To prepare sterile solutions use a teflon or nylon membrane to sterile-filter the DMSO - do not use a cellulose acetate membrane.
Storage & Handling: Room Temperature, desiccate, store under nitrogen
Certifications: Meets ACS specifications