Polyethyleneimine is a branched chain polymer having a ratio of 1:2:1 of primary:secondary:tertiary amines with a branching site every 3 to 3.5 nitrogen atoms and a base structure of (CH₂CH₂NH)x. The nitrogen atoms must be protonated to achieve cationic charge, so the cationic…

n-Butanol is useful for the removal of ethidium bromide from DNA purified by CsCl gradient ultracentrifugation. It is also suitable for use in the concentration of dilute nucleic acid solutions by repeated extractions. Increased nucleic acid concentration improves recovery by ethanol precipitation.

Trichloroacetic acid is an acetic acid analogue commonly used to precipitate proteins, DNA and RNA. In the presence of SDS Trichloroacetic acid will precipitate proteins which can then be quantified by the Lowry method. The compound has also found uses as a decalcifier and fixative in microscopy,…

Propylene glycol is a clear, colorless and hygroscopic liquid. Propylene glycol contains an asymmetrical carbon atom, so it exists in two stereoisomers. The freezing point of water is depressed when mixed with propylene glycol due to increased opportunity for hydrogen bonding. Propylene glycol…

Role of a balanced salt solution in cell culture is multifaceted and can be divided into four principal functions: Serves as an irrigating, transporting and diluting fluid while maintaining intra- and extracellular osmotic balance Provides cells with water and certain bulk inorganic ions…

Isoamy alcohol is routinely used in molecular biology, especially in the purification of DNA. It is widely used in conjunction with phenol and chloroform (25:24:1 phenol : chloroform : isoamyl alcohol) for the removal of proteins from the nucleic acid solutions by extraction. Isoamyl alcohol is…

Eosin Y, a red xanthene dye, has been noted to reduce the affinity of Mg2+- ATPase for ATP and increase the enzyme affinity for Mg2+ at concentrations of 10-50 μM. At higher concentrations near 100 μM the affinity for Mg2+- ATPase for the ion-activator has been shown to be reduced. This…

Ultrapure grade prepared from highly purified acrylamide (4X) and bis-acrylamide (2X) using deionized ultra-filtered water. Store at 4°C and protect from light. Final ratios: 19:1 is optimal for sequencing gels and also for separation of peptides 29:1 for sequencing gels and the separation…

Ammonium acetate is a chemical compound which is derived from the reaction of ammonia and acetic acid. Ammonium acetate is volatile at low pressures. Because of which it has been used to replace cell buffers with non-volatile salts, in preparing samples for mass spectrometry. It is used as a buffer…

Hexane is used in laboratory to extract oil and grease contaminants from water and soil for analysis.

TRITON X-114 is used in biochemical applications to solubilize and separate proteins due to its low cloud point (23 °C). At temperatures above the cloud point, detergent solutions separate into aqueous and detergent-enriched phases. This makes Triton X-114 particularly useful in…

Hexadecane (n-Hexadecane) is an aliphatic long-chain hydrocarbon. It is a reference fuel with the cetane number 100 and is employed in determining the cetane number of diesel fuels. The mechanism of the oxidation of hexadecane in the gas-phase has been described. The density, speed of sound,…

2-Merceptoethanol is used to reduce disulfide bonds and can act as a biological antioxidant by scavenging hydroxyl radicals. 2-Merceptoethanol is used to reduce disulfide linkages in solubilizing proteins for gel electrophoresis. 2-mercaptoethanol is readily oxidized in air to a disulfide,…

Murashige and Skoog Medium is widely used plant tissue culture growth medium. It has been proven effective in the culture of tissue derived from moncotyledons and dicotyledons. This medium, originally formulated to support tobacco callus, has been used to support callus initiation and growth, the…

A mineral oil is any of various colorless, odorless, light mixtures of alkanes in the C15 to C40 range from a non-vegetable (mineral) source, particularly a distillate of petroleum.Most often, mineral oil is a liquid by-product of the distillation of petroleum to produce gasoline and other…

Xylene is used in Giemsa cell staining protocols. Xylene is used as a solvent in pharmaceutical, printing, rubber, and leather industries. It is an important component of ink, rubber and adhesives as well as in thinning paints and varnishes. It is used in the laboratory to make baths with dry ice…

TMB is a chromogenic substrate for Horse Radish Peroxidase (HRP). MP Biolomedicals' TMB Solution is a convenient, non toxic and ready to use stable chromogen solution suitable for peroxidase and pseudo-peroxidase reactions in ELISA, it provides consistent performance and sensitivity to deliver…

1,4 Butanediol and its derivatives are used in the manufacturing of technical plastics, polyurethanes, solvents, electronic chemicals and elastic fibers.

2-Mercaptoethanol is a hybrid of ethylene glycol and 1,2-ethanedithiol. It is used to reduce disulfide bonds and can act as a biological antioxidant by scavenging hydroxyl radicals. It is widely used because the hydroxyl group confers solubility in water and lowers the volatility. Due to its…

Murashige and Skoog Medium contains Murashige and Skoog salts, as well as a mixture of vitamins. This modification of the original formula does not contain agar. Murashige and Skoog Medium is widely used plant tissue culture growth medium.

Xylenes are aromatic hydrocarbons. Xylene is used as a solvent. It is used in the laboratory to make baths with dry ice to cool reaction vessels, and as a solvent to remove synthetic immersion oil from the microscope objective in light microscopy.

Glycerol is a simple polyol compound which is a viscous liquid that is widely used in pharmaceutical formulations. It has three hydroxyl groups that are responsible for its solubility in water and its hygroscopic nature. It is a trihydroxy sugar alcohol that is an intermediate in carbohydrate and…

Premixed liquid, 10X concentrate. Contains 0.25M TRIS base, 1.92M Glycine, pH 8.5. Provides excellent separation of native large molecular weight proteins on TRIS-glycine polyacrylamide gels.

Glycerol is a simple polyol compound. Glycerol is used both in sample preparation and gel formation for polyacrylamide gel electrophoresis. Glycerol (5-10%) increases the density of a sample so that the sample will layer at the bottom of a gel’s sample well. Glycerol is also used to aid…

Ethyl acetate is used primarily as a solvent and diluent. Ethyl Acetate is suitable for HPLC, spectrophotometry, environmental testing.

Polysorbate 20 is typically used as an emulsifying agent for the preparation of stable oil-in-water emulsions, particularly in pharmaceutical applications. It has been used in pre-extraction of membranes to remove peripheral proteins. It has been used as a blocking agent on nitrocellulose. …

2,2'-Azino-bis-(3-ethylbenzthiazoline-6-sulfonic Acid) Solution is oxidized to a radical action showing absorption maxima at 820 nm, 734 nm, 650 nm and 405 nm, in the presence of hydrogen peroxide and HRP. The latter frequency demonstrates a significant molar extinction coefficient and is…

Dimethyl sulfoxide (DMSO) is a highly polar aprotic organic reagent that has exceptional solvent properties for organic and inorganic chemicals. Dimethyl Sulfoxide is ideal for use as a cryoprotectant. Also used in chemical reactions, in polymerase chain reactions (PCR) as a PCR cosolvent to…

This colorless liquid is the simplest organic nitrile (hydrogen cyanide is a simpler nitrile, but the cyanide anion is not classed as organic). It is produced mainly as a byproduct of acrylonitrile manufacture. It is used as a polar aprotic solvent in organic synthesis and in the purification of…

Dimethyl sulfoxide is a dipolar, aprotic solvent. Has been shown to accelerate strand renaturation (1-10% concentration) and is believed to give the nucleic acid thermal stability against depurination. Dimethyl sulfoxide is used to enhance dermal absorption of many chemicals. A solvent for many…

Convenient ready-to-use solutions of 4X high quality acrylamide for molecular biology, and NN’-methylenebisacrylamide in deonized water. Concentration is based on the total weight of both the acrylamide and bis-acrylamide.

Acetone is a commonly used material in the manufacture of various chemicals like methyl isobutyl ketone, mesityl oxide, diacetone alcohol, acetic acid (via the ketene process), iodoform, and bromoform. Other products where acetone is utilized in manufacture include rayon, photographic films, and…