Glycerol is a simple polyol compound.
Glycerol is used both in sample preparation and gel formation for polyacrylamide gel electrophoresis. Glycerol (5-10%) increases the density of a sample so that the sample will layer at the bottom of a gel’s sample well. Glycerol is also used to aid in casting gradient gels and as a protein stabilizer and storage buffer component. It is used in the concentration and storage of enzymes and also prevents back-diffusion of protein samples into the buffer.
Key Applications: Sample preparation in PAGE, Gel formation in PAGE
Application Areas: Molecular Biology
Product Type: Solvents
Chemical Class: Polar
Density: 1.2613 g/cm3 at 20°C (Lit.)
Auto Ignition: 392.78°C (Lit.)
Boiling Point: 166.1°C at 13.3 hPa (Lit.)
Melting Point: 20°C(lit.)
Vapor Density: 3.17 (air = 1)(Lit.)
Vapor Pressure: .0033 hPa at 50°C(Lit.)
Refractive Index: n20/D 1.474 (Lit.)
pH: 5.0 -7.5 (1% aq soln)
Detection Method: FTIR
Storage & Handling: Store at Room Temperature (15-30°C)