MP Biomedicals Buffer Solutions

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MP Biomedicals

MP Biomedicals is a worldwide corporation manufacturing and distributing systems, kits and reagents for the life science research, medical device, biopharma manufacturing and diagnostics markets. Our extensive portfolio and custom services deliver the dependability that research and industrial communities rely on to worry less and discover more. From small scale research to large scale manufacturing, MP Biomedicals has been advancing life science projects for over 50 years.


  • Triethanolamine is a zwitterionic buffer. Triethanolamine(TEA) is used primarily as an emulsifier and surfactant. It neutralizes fatty acids, adjusts and buffers the pH, and solubilises oils and other ingredients that are not completely soluble in water. Triethanolamine is also used as organic…

  • PIPES is frequently used as a buffering agent in biochemistry; it is an ethanesulfonic acid buffer developed by Good et al. in the 1960s. PIPES has a pKa near the physiological pH which makes it useful in cell culture work.

  • Tris has been useful as buffers in a wide variety of biological systems. Uses include pH control in vitro and in vivo for body fluids and in buffering systems for electrophoresis applications. Tris has been used as a starting material for polymers, oxazolones (with carboxylic acids) and…

  • MP Biomedicals

    TBE buffer is a solution of 1M Tris, 0.9M boric acid and 0.01M EDTA disodium salt, prepared from MP's Ultra Pure products. TBE running buffer is the most commonly used buffer for DNA and RNA polyacrylamide gel electrophoresis. TBE is used with non-denaturing or denaturing (7 M urea) gels.…

  • HEPES is a zwitterionic organic chemical buffering agent; one of the twelve Good's buffers. HEPES is widely used in cell culture, largely because it is better at maintaining physiological pH despite changes in carbon dioxide concentration (produced by cellular respiration) when compared to…

  • MP Biomedicals

    Bis-Tris is zwitterionic buffer, an amine buffer similar to Tris. It exhibits only a very small shift in dissociation constant with respect to temperature. Hydrochloric acid can be used to adjust pH for a Bis-Tris buffer to the desired pH.

  • MP Biomedicals

    PIPES is a zwitterionic N-substituted aminosulfonic acid "Good" buffer. Buffers can be made by adding a solution of base to PIPES free acid, titrating to the appropriate pH, or by mixing equimolar solutions of the sesquisodium salt and the disodium salt or dipotassium salt, titrating to…

  • MP Biomedicals

    HEPES is a zwitterionic organic chemical buffering agent; one of the twelve Good's buffers. HEPES is widely used in cell culture largely because it is better at maintaining physiological pH despite changes in carbon dioxide concentration (produced by cellular respiration) when compared to…

  • MP Biomedicals

    SSC Buffer is used in electrophoresis and in sedimentation studies of DNA-histone aggregates. SSC is used as a hybridization buffer in immunoblotting and DNA Microarray protocols. Molecular Biology Grade

  • MP Biomedicals

    BES is a zwitterionic buffer that is used in biochemistry and molecular biology research in the pH range of 6.15 - 8.35 for wide applicability to biochemical studies. A protocol has been reported for the use of BES buffered saline in the calcium phosphate mediated transfection of eukaryotic cells…

  • Tricine was first prepared by Good for use as a buffer for chloroplast reactions. It is structurally similar to Tris, but is much less inhibitory at high concentrations. For ATP assays using firefly luciferase, tricine buffer at 25 mM was found to be the best of ten common buffers tested. …

  • MP Biomedicals

    MES is a zwitterionic buffer. One of the "Good" buffers developed for biological applications. It has the advantages of: midrange pKa, maximum water solubility and minimum solubility in all other solvents, minimal salt effects, minimal change in pK with temperature chemically and…

  • MP Biomedicals

    Tris is an excellent biochemical and biological buffer for all applications where ultra high purity is required.

  • MP Biomedicals

    Tris and Tris Hydrochloride have been useful as buffers in a wide variety of biological systems. Uses include pH control in vitro and in vivo for body fluids and in buffering systems for electrophoresis applications. Tris has been used as a starting material for polymers, oxazolones (with…

  • MP Biomedicals

    Borax anhydrous is typically used as a buffer component. The Merck Index lists other uses as: used in the manufacturing of glazes and enamels, a component in tanning agents; curing and preserving skins, an agent used in cleaning compounds, used to artificially age wood, as a preservative, either…

  • MP Biomedicals

    Used as a buffer in biological research. HEPPSO is an efficient separator in protein resolution by IEF. HEPPSO is a Zwitterionic hydrogen buffer.

  • HEPES is a zwitterionic organic chemical buffering agent. HEPES is widely used in many biochemical reactions and as a buffering agent in some cell culture media.

  • MP Biomedicals

    Tris is relatively non-hygroscopic; however, if needed, Tris Base can be dried at 100°C for up to 4 hours to remove any water. Neither Tris Base or Tris Hydrochloride by themselves provide adequate buffering capacity. Generally the two need to be mixed together to provide a buffer with pH…

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