BES is a zwitterionic buffer that is used in biochemistry and molecular biology research in the pH range of 6.15 - 8.35 for wide applicability to biochemical studies. A protocol has been reported for the use of BES buffered saline in the calcium phosphate mediated transfection of eukaryotic cells with plasmid DNA. BES, a sulfonic acid-containing cross-linking agent, induces cross-linking between sulfonated polyimide chains in sulfonated polyimide membranes.
The effect of BES and other zwitterionic buffers on the Bicinchoninic acid (BCA) measurement of microgram quantities of protein has been studied. BES was found to diminish color development of the proteinBCA complex by 30-35% at 50 mM, and by <10% at 5 mM, measuring in the 1-10 mg range of BSA against protein-BCA complexes formed in water. This color loss may result from binding of Cu2+ to BES.
BES may be employed as binding buffer in modified Eagle′s medium during the binding assay of human melanoma cells. It may be used as biobuffer to investigate the aqueous medium self-assembly of heterometallic CuII/Li 3D coordination polymers.
Purity: ≥99%
Key Applications: Buffer
Product Type: Biochemicals
Biochemical Category: Buffers
Chemical Class: Biochemicals > Buffers
Melting Point: 151- 159°C
Presentation: White Powder
Format: Crystalline
pH: 3-5, Useful pH range-6.4-7.8
pKa: 7.1 at 25°C
Solubility: Soluble 0.1M in water (clear, colorless solution)
Storage & Handling: Store at Room Temperature (15-30°C)