Buffers

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  • Boric Acid

    MP Biomedicals

    Boric acid has a single boron; its salt forms complex equilibria in solution, with the ion B4O7-2 being the predominant species. Boric acid is used for weatherproofing wood and fireproofing fabrics; as a preservative; manufacturing of cements, crockery, porcelain, enamels, glass, borates,…

  • Tris has been useful as buffers in a wide variety of biological systems. Uses include pH control in vitro and in vivo for body fluids and in buffering systems for electrophoresis applications. Tris has been used as a starting material for polymers, oxazolones (with carboxylic acids) and…

  • Sodium Acetate

    MP Biomedicals

    Sodium acetate is the sodium salt of acetic acid. Sodium acetate is used as a buffer in the buffering range of pH 3.6-5.6. It is also used in the purification and precipitation of nucleic acids, protein crystallization, Staining of gels in protein gel electrophoresis, HPLC, mordant dyeing, DNA…

  • Borax, Anhydrous

    MP Biomedicals

    Borax anhydrous is typically used as a buffer component. The Merck Index lists other uses as: used in the manufacturing of glazes and enamels, a component in tanning agents; curing and preserving skins, an agent used in cleaning compounds, used to artificially age wood, as a preservative, either…

  • Sodium Phosphate Dibasic

    MP Biomedicals

    Sodium dibasic anhydrous is used as a sequestrant, emulsifier and buffer in foods, as mordant in dyeing for weighting silk, in tanning, in manufacturing of enamels, ceramics, detergents, boiler compounds; as fireproofing agent in soldering and brazing instead of borax; as reagent and buffer in…

  • Hepes

    MP Biomedicals

    HEPES is a zwitterionic organic chemical buffering agent; one of the twelve Good's buffers. HEPES has been utilized extensively for research and commercial applications in the cell culture and tissue culture industries. HEPES is widely used in cell culture, largely because it is better at…

  • Potassium phosphate is a reagent with high buffering capacity. It occurs in several forms: monobasic, dibasic, and tribasic (K3PO4). Most pH neutral potassium phosphate buffer solutions consist of mixtures of the monobasic and dibasic forms to varying degrees, depending on the desired pH. …

  • Tris-Borate-EDTA Buffer

    MP Biomedicals

    TBE buffer is a solution of 1M Tris, 0.9M boric acid and 0.01M EDTA disodium salt, prepared from MP's Ultra Pure products. TBE running buffer is the most commonly used buffer for DNA and RNA polyacrylamide gel electrophoresis. TBE is used with non-denaturing or denaturing (7 M urea) gels.…

  • HEPES is a zwitterionic organic chemical buffering agent; one of the twelve Good's buffers. HEPES is widely used in cell culture, largely because it is better at maintaining physiological pH despite changes in carbon dioxide concentration (produced by cellular respiration) when compared to…

  • PIPES

    MP Biomedicals

    PIPES is a zwitterionic N-substituted aminosulfonic acid "Good" buffer. Buffers can be made by adding a solution of base to PIPES free acid, titrating to the appropriate pH, or by mixing equimolar solutions of the sesquisodium salt and the disodium salt or dipotassium salt, titrating to…

  • Tricine

    MP Biomedicals

    Tricine, a zwitterionic buffer, was first prepared by Good for use as a buffer for chloroplast reactions. It is structurally similar to Tris, but is much less inhibitory at high concentrations. The name tricine comes from tris and glycine from which it was derived. Tricine can be used in…

  • HEPES is a zwitterionic organic chemical buffering agent. HEPES is widely used in many biochemical reactions and as a buffering agent in some cell culture media.

  • TAE buffer (10X), 1 L

    MP Biomedicals

    Premixed liquid, 10X concentrate. Contains 0.4M TRIS base, 0.4M Acetate, 10mM EDTA, pH 8.0. Used in agarose electrophoresis for separation of nucleic acids, DNA and RNA. Has lower buffer capacity than TBE but double-stranded DNA runs faster in TAE buffer.

  • MES Monohydrate

    MP Biomedicals

    MES is a zwitterionic buffer. One of the "Good" buffers developed for biological applications. It has the advantages of maximum water solubility and minimum solubility in all other solvents, minimal salt effects, minimal change in pK with temperature, chemically and enzymatically stable,…

  • MOPS Sodium Salt

    MP Biomedicals

    MOPS is used as a biological buffer. MOPS is a zwitterionic amino acid which acts as one of the "Good" buffers. It is a structural analog to MES; however, MOPS is a more suitable biological buffer than MES because the pH range is closer to pH 7.4. A buffer using MOPS sodium salt can be…

  • Bis-Tris

    MP Biomedicals

    Bis-Tris is zwitterionic buffer, an amine buffer similar to Tris. It exhibits only a very small shift in dissociation constant with respect to temperature. Hydrochloric acid can be used to adjust pH for a Bis-Tris buffer to the desired pH.

  • MES Monohydrate

    MP Biomedicals

    MES is a zwitterionic buffer. One of the "Good" buffers developed for biological applications. It has the advantages of: midrange pKa, maximum water solubility and minimum solubility in all other solvents, minimal salt effects, minimal change in pK with temperature chemically and…

  • Triethanolamine is a zwitterionic buffer. Triethanolamine(TEA) is used primarily as an emulsifier and surfactant. It neutralizes fatty acids, adjusts and buffers the pH, and solubilises oils and other ingredients that are not completely soluble in water. Triethanolamine is also used as organic…

  • MOPS

    MP Biomedicals

    MOPS is a zwitterionic amino acid which acts as one of the "Good" buffers. It is a structural analog to MES. However, MOPS is a more suitable biological buffer than MES because the pH range is closer to pH 7.4.

  • SSC 20X Buffer

    MP Biomedicals

    SSC Buffer is used in electrophoresis and in sedimentation studies of DNA-histone aggregates. SSC is used as a hybridization buffer in immunoblotting and DNA Microarray protocols. Molecular Biology Grade

  • MOPS

    MP Biomedicals

    MOPS is a zwitterionic amino acid which acts as one of the "Good" buffers. Concentrations higher than 20 mM should not be used with mammalian cell cultures.

  • Tris, Reagent Grade

    MP Biomedicals

    Tris is relatively non-hygroscopic; however, if needed, Tris Base can be dried at 100°C for up to 4 hours to remove any water. Neither Tris Base or Tris Hydrochloride by themselves provide adequate buffering capacity. Generally the two need to be mixed together to provide a buffer with pH…

  • Agar is a polysaccharide complex extracted by bleaching and hot water treatment of agarocytes from the red alga Rhodophyceae, and usually consisting of the genera Gelidium, Acanthopeltis, Ceramium,Pterocladia and Gracilaria. The algae are typically found in the Pacific and Indian Oceans and in the…

  • Tris, Ultra Pure

    MP Biomedicals

    Tris have been useful as buffers in a wide variety of biological systems. It has been used as a starting material for polymers, oxazolones (with carboxylic acids) and oxazolidines (with aldehydes). It does not precipitate calcium salts and is of value in maintaining solubility of manganese salts.…

  • Tricine was first prepared by Good for use as a buffer for chloroplast reactions. It is structurally similar to Tris, but is much less inhibitory at high concentrations. For ATP assays using firefly luciferase, tricine buffer at 25 mM was found to be the best of ten common buffers tested. …

  • HEPES Sodium Salt

    MP Biomedicals

    HEPES is a zwitterionic organic chemical buffering agent; one of the twelve Good's buffers. HEPES is widely used in cell culture largely because it is better at maintaining physiological pH despite changes in carbon dioxide concentration (produced by cellular respiration) when compared to…

  • HEPPSO

    MP Biomedicals

    Used as a buffer in biological research. HEPPSO is an efficient separator in protein resolution by IEF. HEPPSO is a Zwitterionic hydrogen buffer.

  • Bis-Tris

    MP Biomedicals

    Bis-Tris is a zwitterionic buffer; an amine buffer similar to Tris Hydrochloric acid that exhibits only a very small shift in dissociation constant with respect to temperature. It is structurally analogous to the the Good buffers that were developed to provide buffers in the pH range of 6.15 - 8.35…

  • Tris, USP Grade

    MP Biomedicals

    Tris is an excellent biochemical and biological buffer for all applications where ultra high purity is required.

  • SM buffer (sterile solution)

  • PIPES

    MP Biomedicals

    PIPES is a zwitterionic N-substituted aminosulfonic acid "Good" buffer. PIPES is used to buffer glutaraldehyde fixative solutions when fixing plant and/or animal tissue samples. This will help prevent the loss of lipids. It is also used to buffer glutaraldehyde and formaldehyde when…

  • BES

    MP Biomedicals

    BES is a zwitterionic buffer that is used in biochemistry and molecular biology research in the pH range of 6.15 - 8.35 for wide applicability to biochemical studies. A protocol has been reported for the use of BES buffered saline in the calcium phosphate mediated transfection of eukaryotic cells…

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