Buffers

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  • Potassium Phosphate Dibasic

    MP Biomedicals

    Potassium phosphate is a reagent with high buffering capacity. It occurs in several forms: monobasic, dibasic, and tribasic (K3PO4). Most pH neutral potassium phosphate buffer solutions consist of mixtures of the monobasic and dibasic forms to varying degrees, depending on the desired pH. …

  • Tris, Molecular Biology Reagent

    MP Biomedicals

    Tris has been useful as buffers in a wide variety of biological systems. Uses include pH control in vitro and in vivo for body fluids and in buffering systems for electrophoresis applications. Tris has been used as a starting material for polymers, oxazolones (with carboxylic acids) and…

  • Tris-Borate-EDTA Buffer

    MP Biomedicals

    TBE buffer is a solution of 1M Tris, 0.9M boric acid and 0.01M EDTA disodium salt, prepared from MP's Ultra Pure products. TBE running buffer is the most commonly used buffer for DNA and RNA polyacrylamide gel electrophoresis. TBE is used with non-denaturing or denaturing (7 M urea) gels.…

  • Borax

    MP Biomedicals

    Borax is a salt of boric acid which is used as a buffer. It has a co-complexing ability with other agents in water to form complex ions with various substances. It is also commonly utilized in chromatography and electrophoresis studies. Borax is typically used as a buffer component,…

  • HEPES Sodium Salt

    MP Biomedicals

    HEPES is a zwitterionic organic chemical buffering agent; one of the twelve Good's buffers. HEPES is widely used in cell culture largely because it is better at maintaining physiological pH despite changes in carbon dioxide concentration (produced by cellular respiration) when compared to…

  • 1M HEPES Buffer

    MP Biomedicals

    A buffer solution of HEPES can be prepared by any of several methods. The free acid can be added to water, then titrated with approximately one-half mole equivalent of sodium hydroxide or potassium hydroxide to the desired pH. A simple mixing table for preparing 0.05 M HEPES/NaOH has been…

  • Hepes

    MP Biomedicals

    HEPES is a zwitterionic organic chemical buffering agent; one of the twelve Good's buffers. HEPES has been utilized extensively for research and commercial applications in the cell culture and tissue culture industries. HEPES is widely used in cell culture, largely because it is better at…

  • MOPS Sodium Salt

    MP Biomedicals

    MOPS is used as a biological buffer. MOPS is a zwitterionic amino acid which acts as one of the "Good" buffers. It is a structural analog to MES; however, MOPS is a more suitable biological buffer than MES because the pH range is closer to pH 7.4. A buffer using MOPS sodium salt can be…

  • Tris, Ultra Pure

    MP Biomedicals

    Tris have been useful as buffers in a wide variety of biological systems. It has been used as a starting material for polymers, oxazolones (with carboxylic acids) and oxazolidines (with aldehydes). It does not precipitate calcium salts and is of value in maintaining solubility of manganese salts.…

  • MOPS

    MP Biomedicals

    MOPS is a zwitterionic amino acid which acts as one of the "Good" buffers. Concentrations higher than 20 mM should not be used with mammalian cell cultures.

  • MES Monohydrate

    MP Biomedicals

    MES is a zwitterionic buffer. One of the "Good" buffers developed for biological applications. It has the advantages of: midrange pKa, maximum water solubility and minimum solubility in all other solvents, minimal salt effects, minimal change in pK with temperature chemically and…

  • Tris Hydrochloride

    MP Biomedicals

    Tris and Tris Hydrochloride have been useful as buffers in a wide variety of biological systems. Uses include pH control in vitro and in vivo for body fluids and in buffering systems for electrophoresis applications. Tris has been used as a starting material for polymers, oxazolones (with…

  • Tricine

    MP Biomedicals

    Tricine, a zwitterionic buffer, was first prepared by Good for use as a buffer for chloroplast reactions. It is structurally similar to Tris, but is much less inhibitory at high concentrations. The name tricine comes from tris and glycine from which it was derived. Tricine can be used in…

  • Sodium Phosphate Monobasic

    MP Biomedicals

    Sodium phosphate monobasic is a reagent with very high buffering capacity widely used in molecular biology, biochemistry and chromatography. Useful in conjunction with Sodium Dibasic Phosphate, in the preparation of biological buffers. Used in many applications including the purification of antibodies.

  • MES Monohydrate

    MP Biomedicals

    MES is a zwitterionic buffer. One of the "Good" buffers developed for biological applications. It has the advantages of maximum water solubility and minimum solubility in all other solvents, minimal salt effects, minimal change in pK with temperature, chemically and enzymatically stable,…

  • Bis-Tris

    MP Biomedicals

    Bis-Tris is a zwitterionic buffer; an amine buffer similar to Tris Hydrochloric acid that exhibits only a very small shift in dissociation constant with respect to temperature. It is structurally analogous to the the Good buffers that were developed to provide buffers in the pH range of 6.15 - 8.35…

  • Potassium Phosphate Monobasic

    MP Biomedicals

    Potassium phosphate is a reagent with high buffering capacity. It occurs in several forms: monobasic, dibasic, and tribasic (K3PO4). Most pH neutral potassium phosphate buffer solutions consist of mixtures of the monobasic and dibasic forms to varying degrees, depending on the desired pH. …

  • Borax, Anhydrous

    MP Biomedicals

    Borax anhydrous is typically used as a buffer component. The Merck Index lists other uses as: used in the manufacturing of glazes and enamels, a component in tanning agents; curing and preserving skins, an agent used in cleaning compounds, used to artificially age wood, as a preservative, either…

  • Sodium Acetate

    MP Biomedicals

    Sodium acetate is the sodium salt of acetic acid. Sodium acetate is used as a buffer in the buffering range of pH 3.6-5.6. It is also used in the purification and precipitation of nucleic acids, protein crystallization, Staining of gels in protein gel electrophoresis, HPLC, mordant dyeing, DNA…

  • Sodium Phosphate Dibasic

    MP Biomedicals

    Sodium dibasic anhydrous is used as a sequestrant, emulsifier and buffer in foods, as mordant in dyeing for weighting silk, in tanning, in manufacturing of enamels, ceramics, detergents, boiler compounds; as fireproofing agent in soldering and brazing instead of borax; as reagent and buffer in…

  • MOPS

    MP Biomedicals

    MOPS is a zwitterionic amino acid which acts as one of the "Good" buffers. It is a structural analog to MES. However, MOPS is a more suitable biological buffer than MES because the pH range is closer to pH 7.4.

  • Protexidase Peroxidase Protective Buffer

    MP Biomedicals

    Protexidase buffer is 500 mL of PBS, pH 7.2, containing 0.5% BSA, antimicrobial preservatives and proprietary stabilizing compounds. This product stabilizes high dilutions of HRP-antibody conjugates, up to 1:16,000 dilution and inhibits microbial growth in dilute conjugates. This protective…

  • Triethanolamine Hydrochloride

    MP Biomedicals

    Triethanolamine is a zwitterionic buffer. Triethanolamine(TEA) is used primarily as an emulsifier and surfactant. It neutralizes fatty acids, adjusts and buffers the pH, and solubilises oils and other ingredients that are not completely soluble in water. Triethanolamine is also used as organic…

  • Agar from Red Alga Rhodophyceae

    MP Biomedicals

    Agar is a polysaccharide complex extracted by bleaching and hot water treatment of agarocytes from the red alga Rhodophyceae, and usually consisting of the genera Gelidium, Acanthopeltis, Ceramium,Pterocladia and Gracilaria. The algae are typically found in the Pacific and Indian Oceans and in the…

  • PIPES

    MP Biomedicals

    PIPES is a zwitterionic N-substituted aminosulfonic acid "Good" buffer. PIPES is used to buffer glutaraldehyde fixative solutions when fixing plant and/or animal tissue samples. This will help prevent the loss of lipids. It is also used to buffer glutaraldehyde and formaldehyde when…

  • Boric Acid

    MP Biomedicals

    Boric acid has a single boron; its salt forms complex equilibria in solution, with the ion B4O7-2 being the predominant species. Boric acid is used for weatherproofing wood and fireproofing fabrics; as a preservative; manufacturing of cements, crockery, porcelain, enamels, glass, borates,…

  • HEPES, Cell Culture Reagent

    MP Biomedicals

    HEPES is a zwitterionic organic chemical buffering agent; one of the twelve Good's buffers. HEPES is widely used in cell culture, largely because it is better at maintaining physiological pH despite changes in carbon dioxide concentration (produced by cellular respiration) when compared to…

  • HEPES, Molecular Biology Reagent

    MP Biomedicals

    HEPES is a zwitterionic organic chemical buffering agent. HEPES is widely used in many biochemical reactions and as a buffering agent in some cell culture media.

  • HEPES Buffer, Cell Culture Grade

    MP Biomedicals

  • Tris, Ultra Pure

    MP Biomedicals

    Tris have been useful as buffers in a wide variety of biological systems. It has been used as a starting material for polymers, oxazolones (with carboxylic acids) and oxazolidines (with aldehydes). It does not precipitate calcium salts and is of value in maintaining solubility of manganese salts.…

  • Tris, Reagent Grade

    MP Biomedicals

    Tris is relatively non-hygroscopic; however, if needed, Tris Base can be dried at 100°C for up to 4 hours to remove any water. Neither Tris Base or Tris Hydrochloride by themselves provide adequate buffering capacity. Generally the two need to be mixed together to provide a buffer with pH…

  • BES

    MP Biomedicals

    BES is a zwitterionic buffer that is used in biochemistry and molecular biology research in the pH range of 6.15 - 8.35 for wide applicability to biochemical studies. A protocol has been reported for the use of BES buffered saline in the calcium phosphate mediated transfection of eukaryotic cells…

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