Tricine was first prepared by Good for use as a buffer for chloroplast reactions. It is structurally similar to Tris, but is much less inhibitory at high concentrations. For ATP assays using firefly luciferase, tricine buffer at 25 mM was found to be the best of ten common buffers tested.
Tricine is used as buffer component for separation of low molecular weight peptides. Tricine can be used in cryopreservation medium for the preservation of tissues and organs. Cryopreservation depends on the physical and chemical characteristics of the preservation medium used. The pH values and pK values for tricine/DMSO mixtures has been reported down to -20°C. Tricine has been found to be an efficient scavenger of hydroxyl radicals in a study of radiation-induced membrane damage. Tricine is typically the buffer of choice in SDS-PAGE systems when separating proteins in the range of 1 to 100 kDa.
A buffer may be prepared by titrating with sodium hydroxide to the desired pH, using about a half-equivalent of NaOH.
Grade: Electrophoresis Grade
Purity: ≥99%
Suitability: Cell culture tested
Product Type: Biochemicals
Chemical Class: Buffers
Melting Point: 179-189°C (dec.)(Lit.)
UV/Visible Absorbance: OD 260 (0.1 M aq soln) ≤0.1. OD 280 ≤0.1
Presentation: White Powder
Format: Powder
pH: 4.5 - 5.5 (1% aq soln); Buffering pH range 7.4-8.8(Lit.)
pKa: 8.1 (25°C)(Lit.)
Heavy Metals: (as Pb) ≤10 ppm
Moisture content: ≤0.5%
NOTES: DpK/DT: -0.021(Lit.); Metal Binding Constants (log K) for 0.1 M solution at 20°C: Mg2+: 1.2 Ca2+: 2.4 Mn2+: 2.7 Cu2+: 7.3(Lit.)
Solubility: Very soluble in water (25% w/v - clear, colorless solution).
Storage & Handling: Store at Room Temperature (15-30°C).