Address bacterial gene expression hurdle of protein insolubility Low-growth temperatures (or cold-adapted technology) enhance protein folding and solubility Cells are derived from the high-performance Agilent BL21-Gold competent cells;Feature chaperonins Cpn60 and Cpn10 to facilitate proper…

The industry standard for high-fidelity PCR, achieve extreme accuracy with downstream apps. Highest fidelity (1 error/2.5 million bp) for the most reliable, error-free PCR Fast extension for 70-80% quicker time-to-results Accurately amplifies up to 19 Kb genomic DNA targets …

Reduce the time is takes to generate a recombinant adenovirus for gene delivery and expression by several weeks 300% more recombinants with specialty competent cells with the AdEasy system;Infects both dividing and non-dividing cells Human proteins expressed using this system are abundant…

XL1-Blue MRF’ and XL1-Blue MR competent cells allow representational cloning of methylated DNA. Transformation efficiency for XL1-Blue MR and XL-1 Blue MRF’ supercompetent cells of =1 x 10^9 transformants/µg Transformation efficiency for XL1-Blue MRF´ Kan…

Produce more uniform mutational spectra during error-prone PCR. Superior mutagenesis over a wide target range of 0.1 kb to 6 kb Easily change mutation frequency by altering the concentration of the input template Delivers efficient mutagenesis rates of 1 to 16 bases per kb;More…

ABLE K Competent Cells for toxic clones reduce copy number 10-fold compared to the XL1-Blue strain. Reduces the copy number of ColE1-derived plasmids (such as pUC and pBluescript® plasmids) 10-fold Decreases level of cloned protein product to increase cell viability …

Brilliant HRM ultra-fast Loci master mix is for high-resolution melt (HRM) analysis. MgCl₂ and dNTPs included at optimized concentrations Freeze thaw stability cuts waste and increases batch-to-batch reliability EvaGreen release-on-demand dye is non-toxic Freeze thaw…

SURE 2 competent cells are ideal for routine cloning of DNA with secondary structures. Lack components of the pathways that catalyze the rearrangement and deletion of nonstandard secondary and tertiary structures Allow for blue-white color screening Replicating eukaryotic DNA in…

Brilliant III Ultra-Fast SYBR qRT-PCR Master Mixes utilize a novel faster-activating hot start method Provides specificity by utilizing a novel faster-activating hot start method that minimizes formation of primer-dimers and other off-target reactions Pre-blended formulations are compatible…

BL21(DE3) competent cells provide nontoxic protein expression and high transformation efficiency. Cloning directly in the strain can save you two days of work on subcloning procedures BL21-gold competent cells feature the high transformation efficiency phenotype (Hte) and have the gene…

One of our fastest, high-performance, ultrasensitive, next generation qPCR master mix reagents for reliable quantification across a wide range of targets and templates. Fast kit with speeds at 40 min per 40 cycles;Kit comes in master mix format and includes hotstart enzyme Low-abundance RNA,…

Brilliant III qRT-PCR Master Mixes are perfect for researchers who want their data faster. More sensitive and faster via the quick-activating hot start and the engineered Taq-mutant Shortens total cycle time by ~60% compared to standard cycling Maintains amplification efficiency, R2,…

qPCR Brilliant II probe MM with ROX Fast kit in master mix format that includes a hotstart enzyme and ROX reference dye;Low-abundance RNA Amplification and detection from total RNA, poly(A+), and synthetic RNA samples Multiplexing capability The Brilliant II qPCR probe master kit with ROX…

The qRT-PCR core reagent kit includes the components necessary to carry out cDNA synthesis and PCR amplification in one tube and one buffer. Individual optimization of qRT-PCR reagent conditions One-step capability Multiplexing capability Low-abundance RNA Amplification and…

Increases specificity and yield of primary PCR amplification products. Increases yield and specificity of primary PCR amplification products Minimizes formation of poorly matched primer-template complexes Add to genomic PCR reactions to destabilize many mismatched primer-template…

Test your qPCR system for errors with the AriaMx SYBR Qualification Plate Kit. Contains one 96-well plate Each well contains 20 OL of qPCR reagents The AriaMx SYBR Qualification Plate Kit is for use in a qualification test on the AriaMx Real-Time PCR System. Running a qualification test is one…

Agilent PfuTurbo Cx HotStart DNA Polymerase combines high fidelity with improved reliability to amplify problematic systems with greater success. Amplifies problematic systems with greater success Combines high fidelity with improved reliability Tolerates varying PCR conditions with…

Dramatically improve protein expression in E. coli by overcoming codon bias Suitable for expressing proteins from all species, including mammals;Ideal for difficult protein expression, especially when codon bias is a problem;Increases transformation efficiency with Hte phenotype;Derived from…

Agilent PCR-grade dNTP and 10x DNA Polymerase Buffers can be used with any PCR application, including real-time quantitative PCR. Optimizes PCR performance Produces high-quality reaction products Withstands multiple freeze-thaw cycles without compromising efficiency Agilent…

Get easy detection of expression with humanized hrGFP, qualitatively assess transfection efficiency Qualitatively assess the transfection efficiency of the producer cell line Determine the viral titer using fluorescence microscopy or by fluorescence activated cell sorting (FACS) Use…

This primer is for use with components of our High-Specificity miRNA QRT-PCR Detection Kit. For use with Agilent Human U6 Forward Primer;Provided as 200 µL aliquot Workflow component of Agilent’s High-Specificity miRNA QRT-PCR Detection Kit The universal reverse primer is…

Enhance gene expression analysis with cell lysis and immediate stabilization. Save time and money by purifying only the most relevant samples after efficiently screening. RNA is stabilized for at least six months to allow sample archival. Multiple product formats offer maximum choice…

AAV-293 cells are an HEK293 cell line optimized for packaging of AAV virions in helper-free systems. AAV-293 cells are derived from the commonly used HEK293 cell line but produce higher viral titers. HEK293 cells are human embryonic kidney cells transformed by sheared adenovirus type 5…

Improve the speed, efficiency and consistency of single-tube RT-PCR processes Reduced PCR cycling times and a one-tube, master mix format that is designed to reduce contamination, increase throughput and save valuable research time. High affinity for primer-template complexes, delivering…

Competent cells used to prepare plasmid or phagemid cells for more accurate analysis High quality competent cells for preparing methylation-free DNA. The SCS110 strain lacks both dam and dcm activity, and DNA propagated in this strain can be digested by methylation-sensitive enzymes such…

Agilent’s AffinityScript Multiple Temperature cDNA synthesis kit delivers high yields of cDNA using a broad range of temperatures for your application. Robust cDNA synthesis across a broad range of temperatures Recommended up to 60 °C for RNA with increased 2 °C…

The high-purity DpnI restriction enzyme is ideal for cloning and Southern blot analysis. High purity enzyme preparation;Includes 10X universal reaction buffer The high-purity DpnI restriction enzyme is ideal for cloning and Southern blot analysis, and used for site-directed mutagenesis.…

Recombination proficient bacterial strain for production of adenovirus plasmid DNA 300% more recombinants with specialty competent cells Recombination proficient bacterial cell strain Infects dividing and non-dividing cells BJ5183 electroporation competent cells are a…

The Zymo-Seq UDI Primer Set contains 12 Unique Dual Index (UDI) primers that can be used to barcode any library containing TruSeq adapters. Indexed libraries are compatible with any Illumina® sequencer. These indexes are compatible with the Zymo-Seq RiboFree Total RNA Library Kit.

Provides the highest efficiency of conversion of RNA to cDNA, fully optimized for qPCR applications. Optimized for real-time quantitative PCR (qPCR) application;Produces cDNA in 15 minutes with linear detection from 3 pg to 3 µg total RNA;Creates up to 12 kb cDNA in 25 minutes or…

Brilliant III qPCR probe master mix with ROX is one of our fastest, high-performance, ultrasensitive, next generation qPCR master mix reagents. Fast kit with speeds at 40 min per 40 cycles Comes in master mix format, including hotstart enzyme and reference dye Low-abundance RNA, down to…

The Brilliant Multiplex qPCR Master Mix allows four-target amplification in one real-time PCR run. Allows researchers to amplify up to four targets in a single real-time PCR reaction Provides sufficient reaction components to accurately quantify low- and high-abundance targets in the same tube…