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Agilent Genomics

XL1-Blue MR & MRF Competent Cells

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NOTE: Due to special handling or shipping requirements, these products will have additional fees added during checkout. Click HERE for a description of what fees might be charged.

XL1-Blue MRF’ and XL1-Blue MR competent cells allow representational cloning of methylated DNA.

Transformation efficiency for XL1-Blue MR and XL-1 Blue MRF’ supercompetent cells of =1 x 10^9 transformants/µg

Transformation efficiency for XL1-Blue MRF´ Kan supercompetent cells of >1 x 10^9 transformants/µg

Transformation efficiency for XL1-Blue MRF’ electroporation competent cells of >1 x 10^10 transformants/µg XL-1

Blue MRF’ and XL-1 Blue MR competent cells allow high efficiency and representational cloning of methylated DNA. All known E. coli K12 restriction systems have been deleted. Use XL1-Blue MRF’ cells when cloning methylated cDNA or genomic DNA, or when cloning methylated PCR products.

When the F’ episome and blue-white screening are unnecessary, use XL1-Blue MR supercompetent cells. When cloning tetracycline-resistant plasmids, use XL1-Blue MRF’ Kan supercompetent cells to select for the F’ episome and provide a more intense blue color for blue-white screening.

This product is only available to U.S. Domestic Customers.

For research use only, not to be used in diagnostic procedures.

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Product Detail
Thomas No.
CHM01R302
Mfr. No.
200229
UN #
UN1845
Description
XL1-Blue MR Supercompetent Cells 5 x 0.2 mL, For use with cloning without the F' episome, = 1 x 10<sup>9</sup> transformants/µg

Domestic Only (Domestic Only)

Packaging Size
5 x 0.2 mL
list price/quantitytotal
$0.00
Thomas No.
CHM01R303
Mfr. No.
200158
UN #
UN1845
Description
XL1-Blue MRF’ Electroporation-Competent Cells 5 x 0.1 mL, Restriction minus for cloning methylated DNA, = 1 x 10<sup>10</sup> transformants/µg

Domestic Only (Domestic Only)

Packaging Size
5 x 0.1 mL
list price/quantitytotal
$0.00
$0.00 (0 Items)
Product is restricted and can only be purchased by customers with a web profile linked to a Thomas Business Account - Click here to login or create your web profile. If you do not have a Thomas Business Account, please Contact Us and someone will respond with details on how to apply for a business account with us.

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