Produce more uniform mutational spectra during error-prone PCR.
Superior mutagenesis over a wide target range of 0.1 kb to 6 kb
Easily change mutation frequency by altering the concentration of the input template
Delivers efficient mutagenesis rates of 1 to 16 bases per kb;More accurate than methods relying on Taq DNA polymerase which skews the representation of random mutant libraries
GeneMorph II Random Mutagenesis Kits produce highly uniform mutational spectra when performing error-prone PCR. This makes achieving random mutagenesis faster and easier. Alternative methods relying on Taq DNA polymerase have shown biases towards mutating A’s and T’s more frequently than G’s and C’s, skewing representation of random mutant libraries.
GeneMorph II kits utilize Mutazyme II DNA polymerase
A novel error-prone PCR enzyme blend with equivalent mutation rates at A’s and T’s vs. G’s and C’s.This method performs targeted random mutagenesis on protein domains and promoter elements, eliminating the need for specific restriction sites.
This product is only available to U.S. Domestic Customers.
For research use only, not to be used in diagnostic procedures.