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Agilent Genomics

BL21(DE3) Competent Cells

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BL21(DE3) competent cells provide nontoxic protein expression and high transformation efficiency.

Cloning directly in the strain can save you two days of work on subcloning procedures

BL21-gold competent cells feature the high transformation efficiency phenotype (Hte) and have the gene encoding endonuclease I (endA) inactivated. Together, these two features allow direct cloning for most protein expression constructs. By cloning directly in the strain, you can save two days of work normally spent on subcloning procedures. The DE3-derivatives of BL21 contain the T7 RNA polymerase gene controlled by the lacUV5 promoter. This all-purpose derivative yields high-level expression and provides easy induction. Use this derivative with nontoxic proteins.

This product is only available to U.S. Domestic Customers.

For research use only, not to be used in diagnostic procedures.

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Product Detail
Thomas No.
CHM01R042
Mfr. No.
200131
UN #
UN1845
Description
BL21(DE3) Competent Cells 5 x 0.2 mL, For non-toxic proteins, encodes T7 RNA polymerase under the control of the lacUV5 promoter. Use with Variflex or Affinity pCAL vectors.

Domestic Only (Domestic Only)

Packaging Size
5 x 0.2 mL
list price/quantitytotal
$0.00
Thomas No.
CHM01R043
Mfr. No.
200132
UN #
UN1845
Description
BL21(DE3) pLysS Competent Cells 5 x 0.2 mL, For both toxic and non-toxic proteins, pLysS codes for T7 lysozyme, a T7 RNA polymerase inhibitor. Use with pET or pCAL vectors.

Domestic Only (Domestic Only)

Packaging Size
5 x 0.2 mL
list price/quantitytotal
$0.00
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Product is restricted and can only be purchased by customers with a web profile linked to a Thomas Business Account - Click here to login or create your web profile. If you do not have a Thomas Business Account, please Contact Us and someone will respond with details on how to apply for a business account with us.

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