Improve the speed, efficiency and consistency of single-tube RT-PCR processes
Reduced PCR cycling times and a one-tube, master mix format that is designed to reduce contamination, increase throughput and save valuable research time.
High affinity for primer-template complexes, delivering optimal cDNA yield.
Combined performance of Herculase II fusion DNA polymerase and the ArchaeMaxx PCR enhancing factor, delivering superior PCR yield and reliability.
Robust performance for difficult and GC-rich targets.;Optimized for target lengths up to 9.5 KB.
The high fidelity of Herculase II fusion DNA polymerase results in low error rates for RT-PCR cloning applications.
The Agilent AffinityScript One-Step RT-PCR Kit provides a complete system for fast, high-yield, reliable single-tube RT-PCR. The system features a master mix format which, when combined with robust and rapid cycling conditions, makes it ideal for gene profiling and other high-throughput end-point RT-PCR applications.
Using the kit cDNA is synthesized from total or poly(A)+ RNA. This is carried out using a genetically engineered, highly thermostable version of MMLV RT called AffinityScript reverse transcriptase (RT). In addition, a special enzyme formulation and the kit's optimized buffer system ensure consistent and robust performance when amplifying difficult and GC-rich targets.
This product is only available to U.S. Domestic Customers.
For research use only, not to be used in diagnostic procedures.