G-Biosciences, Protein Purification Chromatography Media

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  • Prepacked purification columns for purification are available in packs of 5.The columns available are the anionic chromatography columns, hydrophobic columns, size exclusion (gel filtration) columns.

  • An important tool in research is the generation of peptide and protein affinity columns for the purification of antibodies and for the discovery of important interacting proteins and cofactors. G-Biosciences offersa complete kit for the coupling of peptides and proteins to agarose through their…

  • G-Biosciences

    G-Sep™ is a gel filtration resin comprised of ultrapure cross-linked dextran for desalting and buffer exchange in industrial applications. It exhibits high selectivity, superb resolution, low non-specific adsorption and robust chemical stability. Quickly desalts, removes contaminants and…

  • G-Trap™ GT-1200 Desalting Column is prepacked ready to use column for separation of low molecular weight substances from high molecular weight substances based on size exclusion chromatography. G-Trap™ GT-1200 Desalting Columns are mostly used for removal of salt or buffer exchange…

  • G-Biosciences

    Thiophilic adsorption or thiophilic chromatography is a routinely used technique for the low cost, simple purification of immunoglobulins. Thiophilic adsorption was first developed by Porath et al 1 in 1984 and is a group specific, salt-dependent purification technique that has distinct affinity…

  • The 5ml empty, plastic, disposable columns have an internal volume of 6.5ml and is designed for small scale gravity flow purifications. The resin bed volume is 2.5ml. Supplied with caps and stoppers. Features Total Volume: 6.5ml Resin Volume: 2.5ml Reservoir Volume: 4ml …

  • Our 20mL empty, plastic, disposable columns, designed for small scale gravity flow purifications, have an internal volume of 32mL and a resin bed volume of 20mL.  The columns are supplied with screw caps and stoppers. Features Total Volume: 32mL Resin Volume: 20mL Reservoir…

  • Lectins are proteins or glycoproteins that have at least one noncatalytic domain that binds specifically and reversibly to monosaccharides or oligosaccharides. Lectins are important as they are found in applications in science, medicine, agriculture, pharmaceutical companies, biotechnology and…

  • G-Biosciences

    Immobilized Metal Ion Affinity Chromatography (IMAC), developed by Porath (1975), is based on the interaction of certain protein residues (histidines, cysteines, and to some extent tryptophans) with cations of transition metals. The Ni-NTA Resin is specifically designed for the purification of…

  • G-Trap™ Phenyl Agarose 6 Fast Flow (High Sub) are prepacked ready to use columns for seperation of biomolecules via Hydrophobic Interaction chromatography (HIC). Biomolecules are separated on the HIC column based upon the hydrophobicity of the ligand attached to the resin, the…

  • G-Biosciences

    Immobilized Metal Ion Affinity Chromatography (IMAC), developed by Porath (1), is based on the interaction of certain protein residues (histidines, cysteines, and to some extent tryptophans) with cations of transition metals. The Cobalt Chelating Resin is specifically designed for the…

  • G-Biosciences

    Immobilized Procainamide is designed for affinity purification of butyrylcholinesterase (BuChE), which is also known as non-specific cholinesterase. Butyrylcholinesterase can be purified by batch adsorption on immobilized Procainamide followed by ion-exchange chromatography on DEAE-Sepharose…

  • G-Trap™ ProteinG are prepacked ready to use columns for affinity purification of Immunoglobulins G. The column is packed with resin, which is covalently coupled to recombinant Protein G. Recombinant Protein G is coupled covalently by proprietary coupling method to highly crosslinked 6%…

  • G-Sep™ Phenyl Agarose Fast Flow (FF) is a 6% cross-linked agarose with phenyl groups for Hydrophobic Interaction Chromatography (HIC). The resin is used to separate biomolecules on the basis of relative hydrophobicity. G-Sep™ Phenyl Agarose Fast Flow is available in two levels of…

  • G-Biosciences

    Reducing agents are used in the reduction of disulfide bonds of proteins and peptides. It is often necessary to remove the reducing agents from the protein/peptide solutions to prevent them from interfering with subsequent procedures. However, for small proteins, and particularly peptides, it is…

  • G-Biosciences CDI Amine Reactive Agarose consists of 6% cross-linked agarose activated with CDI (1,1’-carbonyl diimidazole) to form reactive imidazole carbamates. The activation of the resin occurs in solvent and to maintain its activity the resin is supplied in acetone to prevent…

  • Immobilized Protein A/G consists of recombinant protein A/G ligand covalently immobilized onto 6% highly cross-linked agarose.The dynamic binding capacity will vary depending on several factors such as target antibody, flow rate etc. Protein A/G binds well to IgG subclasses but does not bind…

  • G-Trap™ SP Agarose Fast Flow is ready to use prepacked column used for seperation of proteins and other biomolecules based on charge. The resin in G-Trap™ SP Agarose Fast Flow is a strongcation exchanger composed of highly crosslinked 6% agarose beads coupled with sulphopropyl (SP)…

  • Immobilized protein G is for binding the constant domains of immunoglobulin (Ig) molecules (see Figure 1). It is ideal for the easy one-step purification of classes, subclasses and fragments of immunoglobulins for biological fluids and cell culture media. Protein G is a modified form of…

  • Epoxy-Activated Agarose is preactivated resin used for affinity chromatography. Epoxy-Activated Agarose comprise of high density epoxy groups that form covalent bonds with ligand s containing amine groups, thiol or hydroxyl groups. Epoxide-Acivated Agarose is prepared by immobilization of…

  • The Carbohydrate Coupling Resin is designed for the simple and efficient coupling of glycoproteins to a solid agarose support through oxidized sugar groups. The coupling is through the hydrazide group that is immobilized to agarose beads. The resin is ideal for immobilized polyclonal antibodies as…

  • G-Sep™ DEAE Agarose Fast Flow (FF) resin is a weak anion exchanger composed of highly cross-linked 6% agarose beads, with diethylaminoethyl (DEAE) weak anion exchange groups. The resin is extensively used for biomacromolecule separations in both research and industrial applications. The…

  • G-Trap™ Butyl Agarose 6 Fast Flow are prepacked ready to use columns for seperation of biomolecules via Hydrophobic Interaction chromatography (HIC). Biomolecules are separated on the HIC column based upon the hydrophobicity of the ligand attached to the resin, the distribution of…

  • G-Trap™ Octyl Agarose 6 Fast Flow are prepacked ready to use columns for seperation of biomolecules via Hydrophobic Interaction chromatography (HIC). Biomolecules are separated on the HIC column based upon the hydrophobicity of the ligand attached to the resin, the distribution of…

  • NHS-Activated Agarose consists of 4% cross-linked agarose that has been activated by the addition of a reactive NHS (N-hydroxysuccinimide) group. The NHS group forms covalent, chemically stable amide bonds with ligand s that contain primary amines. The NHS-Activated Agarose also contains a spacer…

  • G-Trap™ Q Agarose Fast Flow is ready to use prepacked column used for seperation of proteins and other biomolecules based on charge. The resin in G-Trap™ Q Agarose Fast Flow is a strong anionexchanger composed of highly crosslinked 6% agarose beads coupled with quaternary ammonium…

  • G-Sep™ SP Agarose Fast Flow (FF) resin is a strong cation exchanger composed of highly cross-linked 6% agarose beads, with Sulphopropyl (SP) strong cation exchange groups. The resin is extensively used for biomacromolecule separations in both research and industrial applications. The…

  • G-Trap™ NiIDA Agarose Fast Flow columns are ready to use prepacked affinity columns used for purification of proteins based on immobilized metal ion affinity chromatography (IMAC). IMAC is based on interaction of cations of transition metals such as Ni2+, Co2+ with protein residues…

  • G-Trap™ Ion Exchange Selection Kit contains 4 different types of 1 ml G-Trap™ Ion Exchange Agarose Fast Flow Columns which are used for initial screening of the suitable ion exchange resin and /or pH or ionic strength optimization of start and elution buffer to separate the target…

  • G-Biosciences

    Immobilized metal affinity chromatography (IMAC) resin utilizing nickel (Ni 2+ ) for the purification of 6x histidine tagged proteins. This resin binds to six histidine residues (6x His), a common tag used in protein purification. The resin consists of iminodiacetate coupled to 6% cross-linked…

  • Size Exclusion Chromatography or gel filtration is a widely used technique for separation of proteins and other molecules based on their sizes. G-Sep™ Size Exclusion Columns are composed of high performance rigid resin made up of co-polymer of agarose and dextran. The Columns are available…

  • G-Biosciences

    ECH-Agarose contains carboxyl functional group for coupling of affinity ligand s via their amine groups for affinity chromatography. ECH-Agarose is prepared by condensation of Carboxyl Coupling Resin (Cat. # 786-797) with succinic anhydride, resulting in 15-atom hydrophilic spacer arm. Amine…

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