G-Trap™ Phenyl Agarose 6 Fast Flow (High Sub) are prepacked ready to use columns for seperation of biomolecules via Hydrophobic Interaction chromatography (HIC).
Biomolecules are separated on the HIC column based upon the hydrophobicity of the ligand attached to the resin, the distribution of surface-exposed hydrophobic amino acids present on the protein or enzyme to be separated and the concentration and type of salt used in binding or start buffer. In HIC, the binding of biomolecules to the column (ligand ) is facilitated by using higher concentrations of anti-chaotropic salts such as ammonium sulfate, sodium sulphate etc in the binding buffer or start buffer.
Straight alkyl chains ligand s such as butyl, octyl show pure hydrophobic character whereas aryl ligand s such as phenyl show mixed mode behavior where aromatic, hydrophobic as well as lack of charge play role in protein/biomolecules adsorption to the column.
G-Biosciences offer a wide range of G-Trap™ HIC Columnsincluding G-Trap™ Butyl Agarose 6 Fast Flow , G-Trap™ Octyl Agarose 6 Fast Flow , G-Trap™ Phenyl Agarose 6 Fast Flow (High Sub), G-Trap™ Phenyl Agarose 6 Fast Flow (Low Sub ) so that one can choose the HIC column that is suitable for separation of the target protein. In addition G-Trap™ HIC Selection Kit is available for initial screening for appropriate hydrophobic column and purification conditions for the target protein
The properties of G-Trap™ Phenyl Agarose 6 Fast Flow (High Sub) resin and column are listed below
G-Trap™ Phenyl Agarose 6 Fast Flow (High Sub) Resin
Mean particle size: 90 µm
Bead structure: Highly cross-linked 6% Agarose
Ligand : Phenyl
Ligand Concentration: About 40μmol/ml
Binding Capacity: About 40mg HSA/ml resin
pH stability Working Range: 3-13
pH stability Cleaning-in-Place (CIP): 2-14
Maximum Pressure (MPa): 0.3
Maximum Flow Velocity: 450cm/h
Exclusion Limit (Globular Proteins): 4 x 106
Physical Stability: Negligible volume variation due to changes in pH or ionic strength
Chemical Stability: Stable to all commonly used aqueous buffers:1 M NaOH, 8 M urea, 8 M guanidine hydrochloride, 70% ethanol
Storage Conditions: 4 to 30°C, 20% Ethanol
G-Trap™ Phenyl Agarose 6 Fast Flow (High Sub), 1 ml columns
Column Volume: 1 ml
Columns Dimension: 0.7 x 2.5 cm
Column Harware Pressure Limit: 0.5 MPa
Column Hardware: Polypropylene (Biocompatible)
G-Trap™ Phenyl Agarose 6 Fast Flow (High Sub), 5 ml columns
Column Volume: 5 ml
Columns Dimension: 1.6 x 2.5 cm
Column Harware Pressure Limit: 0.5 MPa
Column Hardware: Polypropylene (Biocompatible)
Application(s):
- G-Trap™ Phenyl Agarose 6 Fast Flow (High Sub) are prepacked ready to use columns for separation of biomolecules via Hydrophobic Interaction chromatography (HIC).