G-Trap™ NiIDA Agarose Fast Flow columns are ready to use prepacked affinity columns used for purification of proteins based on immobilized metal ion affinity chromatography (IMAC).
IMAC is based on interaction of cations of transition metals such as Ni2+, Co2+ with protein residues histidine, cysteine and to some extent to tryptophan. In G-Trap™ Ni IDA Agarose Fast Flow , Ni2+ are immobilized on cross-linked 6% agarose using iminodiacetic acid groups (IDA) which are covalently bound to resin.
G-Trap™ Ni IDA Agarose Fast Floware specifically designed for purification of recombinant proteins fused with 6x histidine (6XHis) tag.
Other G-Trap™ IDA Agarose Fast Flow column available in 1 ml and 5 ml column volume is G-Trap™ CoIDA Agarose Fast Flow . One can chose between appropriate column as per requirement.
The properties of G-Trap™ Ni IDA Agarose Fast Flow resin and the Column are listed below.
G-Trap™ Ni IDA Agarose Fast Flowresin
Matrix: Cross-linked agarose beads, 6%
Mean particle size: 90 μm
Chelating Agent: Iminodiacetic acid
Active group: Ni2+
Metal iondensity: 20-40 μmol /ml
Binding Capacity: 5-10mg His-tagged protein/ml medium
pH stability Working Range: 3-12
pH stability Cleaning-in-Place (CIP): 2-14
Maximum Flow Velocity: 2-14
Recommended Flow rate: 1 ml/min and 5 ml/min for 1 ml and 5 ml column respectively
Exclusion limit(globular proteins): 4 x 106
Chemical Stability: Stable to all commonly used aqueous buffers: 8 M urea, 6 M guanidine hydrochloride
Storage Conditions: 2 to 8°C, 20% Ethanol
G-Trap™ Ni IDA Agarose Fast Flow?, 1 ml columns
Column Volume: 1 ml
Columns Dimension: 0.7 x 2.5 cm
Column Harware Pressure Limit: 0.5 MPa
Column Hardware: Polypropylene (Biocompatible)
G-Trap™ Ni IDA Agarose Fast Flow, 5 ml columns
Column Volume: 5 ml
Columns Dimension: 1.6 x 2.5 cm
Column Harware Pressure Limit: 0.5 MPa
Column Hardware: Polypropylene (Biocompatible)
Application(s):
- G-Trap™ Ni IDA Agarose Fast Flow columns are ready to use prepacked affinity columns used for purification of proteins based on immobilized metal ion affinity chromatography (IMAC).G-Trap™ Ni IDA Agarose Fast Flow are specifically designed for purification of recombinant proteins fused with 6x histidine (6XHis) tag.