G-Trap™ ProteinG are prepacked ready to use columns for affinity purification of Immunoglobulins G. The column is packed with resin, which is covalently coupled to recombinant Protein G.
Recombinant Protein G is coupled covalently by proprietary coupling method to highly crosslinked 6% agarose. The coupling chemistry used shows high coupling efficiency and low Protein G leaching and thus high binding efficiency. The recombinant Protein G, produced in E. coli, has been designed to genetically remove albumin and cell surface binding regions of the protein.
G-Trap™ rProtein A FF, 1 ml and G-Trap™ rProtein A FF, 5 ml are also available for affinity purification of Immunoglobulins.
The properties of the column and resin are listed below
G-Trap™ Protein G resin
- Mean particle size: 90 µM
- Bead Structure:Highly cross-linked 6% agarose
- Ligand :Recombinant Streptococcal Protein G lacking the albumin binding domain produced in E. coli
- Binding capacity:38mg human IgG/ml resin ; >20mg sheep IgG/ml resin
- Maximum flow rate:1000 cm/hr
- Chemical Stability:Stable in all commonly used buffers
- pH stability: Long term: pH 3-9, short term pH 2-9
- Storage:2°C to 8°C in 20% ethanol
G-Trap™ Protein G, 1 ml columns
- Column Volume: 1 ml
- Columns Dimension: 0.7 x 2.5 cm
- Column Harware Pressure Limit: 0.5 MPa
- Column Hardware: Polypropylene
G-Trap™ Protein G, 5ml columns
- Column Volume: 5ml
- Columns Dimension: 1.6 x 2.5 cm
- Column Harware Pressure Limit: 0.5 MPa
- Column Hardware: Polypropylene
Application(s):
- G-Trap™ Protein G are prepacked ready to use columns for affinity purification of Immunoglobulins G