Protein
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FABP3 (human recombinant)
Cayman ChemicalSource: recombinant protein N-terminal His-tag expressed in E. coli Mr: 19 kDa Protein concentration: 0.5 mg/ml
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SET8 (human recombinant)
Cayman ChemicalSource: Active recombinant N-terminal His-tagged protein amino acids 190-352, expressed in E. coli Mr: 21.1 kDa SET8 selectively mono-methylates histone H4 at lysine 20, an event proven to have an important role in chromatin structure and transcriptional activation. SET8 is also a novel…
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UHRF1 PHD domain (human recombinant)
Cayman ChemicalSource: Recombinant N-terminal GST-tagged protein expressed in E. coli Mr: 35.5 kDa
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G9a-like protein (human recombinant)
Cayman ChemicalActive recombinant N-terminal GST-tagged protein expressed in E. coli • G9a-like protein (GLP) is a SET domain-containing methyltransferases that specifically mono- and di-methylate histone H3 at lysine 9 (H3K9).
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CBX3 (human recombinant)
Cayman ChemicalSource: Recombinant N-terminal GST-tagged protein expressed in E. coli Mr: 48.4 kDa
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Cholera Toxin (azide free)
Cayman ChemicalA multimeric enterotoxin that catalyzes the ADP-ribosylation of the Gsα protein, which activates adenylate cyclase and increases the intracellular concentration of cAMP; causes the hypersecretion of chloride and bicarbonate ions, which results in increased fluid secretion and the severe…
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Glutaredoxin 1 from E. coli
Cayman ChemicalSource: one ampoule contains salt-free GRX from E. coli Mr: 10 kDa
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GDF11 (human recombinant)
Cayman ChemicalSource: Recombinant protein expressed in E. coli MW: 12.5 kDa
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Diphtheria Toxin (unnicked) from Corynebacterium diphtheriae
Cayman ChemicalAn intact toxin that catalyzes the ADP-ribosylation and inactivation of EF-2, terminating protein synthesis and causing cell death.
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PPARd (human recombinant)
Cayman ChemicalSource: recombinant protein expressed in Sf21 cells Mr: 54 kDa PPARs are members of the nuclear receptor family of ligand activated transcription factors that heterodimerize with retinoic acid like receptors, regulating gene expression and differentiation. PPARδ is a mediator of diverse…
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Streptavidin:HRP
Cayman ChemicalStreptavidin HRP Binding Moiety: Streptavidin Enzyme: Horseradish Peroxidase Uses: Western blot detection. Recommended dilution of 1:500-1:10,000. Starting dilution: 1:2,000.
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Nitrotyrosine BSA
Cayman ChemicalNitrotyrosine BSA is a positive control for detection of protein tyrosine nitration by WB using nitrotyrosine antibodies.
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HDAC3/NCOR2 (human recombinant)
Cayman ChemicalSource: 50 µg of a complex of full length recombinant C-terminal His-tagged HDAC3 and recombinant N-terminal GST-tagged NCOR2 amino acids 395-489, co-expressed in baculovirus expression system Mr: 49.7 kDa (HDAC3); 37.6 kDa (NCOR2) HDAC3 is a Class I HDAC that is inactive alone and…
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Thrombin (human)
Cayman ChemicalSource: Human blood; all source material tested and found non-reactive to HTLV-III and HBsAg using FDA approved assay methods Mr: 38 kDa
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5-Lipoxygenase (human recombinant)
Cayman ChemicalRecombinant enzyme isolated from a Baculovirus overexpression system in Sf21 cells.
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PCSK9 (human recombinant)
Cayman ChemicalSource: Active recombinant N-terminal His-tagged enzyme isolated from a HEK293 overexpression system MW: 71 kDa (~16 & 63 kDa purified) PCSK9 is a member of the subtilisin serine protease family with an important role in lipoprotein metabolism. Mutation in the PCSK9 gene is associated…
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sPLA2 (Type III)
Cayman ChemicalEC 3.1.1.4 Active enzyme isolated from bee venom One unit of enzyme hydrolyzes one µmol of Diheptanoyl Thio-PC per minute at 25°C PLA2 catalyzes the hydrolysis of fatty acids at the sn-2 position of glycerophospholipids, yielding a free fatty acid and a lysophospholipid. PLA2…
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STING (human recombinant)
Cayman ChemicalSource: Recombinant N-terminal His- and SUMOpro-tagged protein expressed in E. coli Mr: 34.5 kDa
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TP53BP1 BRCT domains (human recombinant)
Cayman ChemicalSource: Recombinant human N-terminal GST-tagged protein expressed in E. coli amino acids 1,717-1,972 (N- and C-terminal truncation)
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nNOS Electrophoresis Standard
Cayman ChemicalEach vial contains nNOS (rat) to be used as a standard for WB and electrophoresis. The enzyme has been carefully purified to exclude all unrelated proteins; may not be catalytically active.
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JMJD2E-Strep tagged (human recombinant)
Cayman ChemicalSource: Active recombinant N-terminal Strep II-tagged protein expressed in E. coli Mr: 40.8 kDa JMJD2E catalyzes the demethylation of histone H3 at lysince residue 9. JMJD2E is an α-ketoglutarate-dependent Fe (II) oxygenase. Purification of Fe-dependent JmjC family members by IMAC can…
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Source: Human recombinant PP2A catalytic subunit expressed in insect cells with an N-terminal octahistidine-tag followed by a streptactin-tag. The C-terminal leucine 309 was deleted. MW: 38.6 kDa PP2A is a divalent cation-independent protein serine/threonine phosphatase involved in regulating…
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PTEN (human recombinant)
Cayman ChemicalSource: Active human recombinant N-terminal His-tagged protein purified from Sf21 cells Mr: 50.8 kDa PTEN functions as a key regulatory enzyme in many signal transduction pathways by dephosphorylating proteins and lipids such as Akt and PIP3. Mutation of PTEN results in many human cancers…
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Spectral Characteristics: Visible absorption maximum = 483 nm, Emission maximum = 673 nm; Peridinin Chlorophyll (PerCP), crosslinked with SMCC for stability.
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Annexin A1 (human recombinant)
Cayman ChemicalSource: Recombinant N-terminal His-tagged protein expressed in E. coli MW: 40.48 kDa Annexin A1 is an endogenous mediator of inflammation, promoting resolution in a number of ways. Normally expressed in intracellular compartments, it is drawn to the cell membrane and both induced and…
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Thioredoxin 1 (mouse recombinant)
Cayman ChemicalSource: one ampoule contains 100 µg of salt-free recombinant mouse cytosolic thioredoxin Activity: fully active with mammalian thioredoxin reductase
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Adenosine Receptor A2A Blocking Peptide
Cayman ChemicalAdenosine Receptor A2A is a multi-pass membrane protein that is normally localized to the plasma membrane. This receptor is part of the G-protein coupled receptor family that binds adenosine and keeps it in equilibrium inside and outside the cell. Along with establishing this…
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Melittin
Cayman ChemicalThe principal cytotoxic component of bee venom; assembles as tetramers on membrane surfaces to form pores, resulting in cell death.
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PtdIns-(3,4,5)-P3 Binding Protein
Cayman ChemicalSource: recombinant N-terminal GST-tagged protein purified from E. coli Mr: ~41.7 kDa (15.4 kDa native; 26.3 kDa GST-tagged) PtdIns-(3,4,5)-P3 Binding Protein contains a highly specific PH domain that recognizes and binds PtdIns-(3,4,5)-P3. PtdIns phosphates play a critical role in the…
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BRD4 bromodomain 1 (human recombinant; GST-tagged)
Cayman ChemicalSource: recombinant N-terminal GST-tagged protein expressed in E. coli Mr: 41.4 kDa The isolated individual or tandem bromodomains of BRD4 have been shown to bind acetylated histone tails, serving to couple histone acetylation marks to the transcriptional regulation of target promoters.
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EED (human recombinant)
Cayman ChemicalSource: Recombinant N-terminal His-tagged protein expressed in Sf21 cells Mr: 53.5 kDa EED is a WD40 repeat-containing protein that forms part of the Polycomb repressive complex 2 (PRC2). The EED subunit does not contain methyltransferase activity. However, transcriptional repression by…
