G-Biosciences Buffer Solutions
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RAB Buffer (Reassembly Buffer)
G-BiosciencesA high salt RAB (Reassembly) buffer for the lysis of mammalian cells, including CHO 1,2 , COS 3 , NT2N 4,5 and HEK293 10 ; C. elegans 6,10 and brain tissue 7-9 . Designed for the extraction of soluble proteins, does not extract detergent extractable insoluble proteins. Features …
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SDS-PAGE Running Buffer
G-BiosciencesFeatures SDS-PAGE Running Buffer (10X) Compatible with Tris-Glycine gels Separate proteins in the range 6-200 kDa under denaturing conditions 1 X concentration: 25mM Tris Base, 192 mM Glycine, 0.1% SDS Applications Tris-Glycine/SDS Running Buffer [10X] is compatible…
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Streptavidin Elution Buffer
G-BiosciencesFeatures 8M Guanidine.HCl, pH 1.5 Streptavidin resin also available
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Acetate Buffer
G-BiosciencesAcetate Buffer at pH 5.0 and 5.5 are supplied as ready to use solutions that eliminates the calculation, formulation and weighing errors. Features Precision in pH Guaranteed reproducibility
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Zymogram Sample Buffer
G-BiosciencesZymography is an electrophoretic technique, based on SDS-PAGE, that includes an enzyme substrate copolymerized with the polyacrylamide gel. Samples are prepared in Zymogram Sample Buffer without boiling to preserve the structure and activity of the enzyme. Following electrophoresis, the SDS is…
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PBST [10X]; Phosphate buffered saline with Tween-20
G-BiosciencesA 10X Concentrated solution of Phosphate Buffered Saline with Tween® 20. The 10X Concentration is 80mM Na2HPO4, 1.5M NaCl, 20mM KH2PO4, 30mM KCl, 0.5% Tween® 20, pH 7.4. PBST is commonly used as a wash solution for Western blot…
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SSC [20X] (Sodium Chloride-Sodium Citrate)
G-BiosciencesSaline-Sodium Citrate Hybridization Buffer [20X] controls stringency during the washing steps in Southern blotting, in situ hybridization, DNA microarray or Northern blotting. 20X SSC may be used to prevent the drying of agarose gels during a vacuum transfer. Features …
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TE Buffer [1X]
G-BiosciencesTE Buffer [1X], pH 8.0, Low EDTA (Tris-EDTA; 10mM Tris base, 0.1mM EDTA) are available in different sizes. Other concentrations of TE Buffer are offered, including a 100X concentration and a 10X concentration. 10X and 100X regular TE Buffers are also available Features Low EDTA…
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PBS [10X] (Phosphate Buffered Saline)
G-BiosciencesPBS (Phosphate Buffered Saline) Ready-to-use solutions, available in 1X and 10X concentrated solutions. Features 1X: 8mM NaH2PO4, 150mM NaCl, 3mM KCl, 2mM KH2PO4 (pH 7.4) 10X: 80mM NaH2PO4 1.5M NaCl,…
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Tris
G-BiosciencesTris is an abbreviation of the organic compound known as tris(hydroxymethyl)aminomethane, with the formula (HOCH2)3CNH2. Tris is extensively used in as a major component in buffer solutions. This 1M solution is has a pH of 9.0. Other 1M Tris solutions are…
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TNT Buffer
G-BiosciencesA general molecular biology and hybridization buffer Features 0.1M Tris·HCl (pH 7.5) 0.15M sodium chloride 0.05% Tween® 20
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Features Synonym: 1,4-Piperazinediethanesulfonic acid sesquisodium salt, Piperazine-1,4-bis(2-ethanesulfonic acid) sesquisodium salt CAS#: 100037-69-2 Molecular Formula: …
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IgG Binding, Wash & Elution Buffers
G-BiosciencesG-Biosciences IgG Binding, Wash & Elution Buffers are designed for the non-denaturing, high yield purification of antibodies from IgG affinity purification resins.The buffers are ideal for the purification of antibodies from Protein A , Protein G and Protein A/G resins. IgG Binding…
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STET-E1 Solution
G-BiosciencesFor DNA/RNA Extraction and Purification Features 3M NaCl, 165mM Tris-HCl, pH 8.0, 45mM EDTA, pH 8.0, and 16.4% Tween® 20
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TE Buffer [10X]
G-BiosciencesSynonyms: TE Buffer, Tris-EDTA Buffer, T10E1 Buffer The TE Buffer is a common molecular biology buffer used for protection of DNA and RNA from degradation. The Tris buffering agent and EDTA metal chelating properties help protect DNA and RNA. The TE Buffer [10X] is offered in 0.5 and 1L…
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Trypsin Digestion Buffer
G-BiosciencesThe Trypsin Digestion Mix provides optimal buffered conditions for in gel trypsin digestion of proteins. Suitable for 100 protein spots.
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Sodium Phosphate (Dibasic)
G-BiosciencesFeatures Synonym: Disodium hydrogen phosphate CAS#: 7558-79-4 Molecular Formula: Na2HPO4 Molecular Weight: 231-448-7
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IEF Cathode Buffer (pH 3-10) [10X]
G-BiosciencesIsoelectric Focusing Buffer for Cathode, pH3-10 Features pH 3-10
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TBST [10X]; Tris buffered saline with Tween-20
G-BiosciencesA 10X Concentrated solution of Tris Buffered Saline with Tween® 20. The 10X Concentration is 100mM Tris.HCl, 150mM NaCl, 0.5% Tween® 20 at pH7.5. The 1X Concentration is 10mM Tris.HCl, 15mM NaCl, 0.05% Tween® 20 at pH7.5. TBST…
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ACD (Acid Citrate Dextrose) Solutions
G-BiosciencesACD (Acid Citrate Dextrose) Solution A andACD (Acid Citrate Dextrose)Solution B are anticoagulants for whole blood and also act as acidifying agents to platelet poor plasma. Features Solution A: 38mM citric acid, 107mM sodium citrate, 136mM dextrose Solution B: 23mM citric acid,…
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Sodium Bicarbonate
G-BiosciencesFeatures Synonym: Natron CAS#: 144-55-8 Molecular Formula: NaHCO3 Molecular Weight: 84.01
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FOCUS™ Extraction Buffers
G-BiosciencesChaotropic extraction buffers that preserve the native charge of proteins making them s uitable for sample extraction for 2D gels One of the most important considerations before running 2D gel electrophoresis is the choice of protein solubilization buffers. A suitable buffer must…
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GST Binding/Wash Buffer (PBS + 1% Triton® X-100)
G-BiosciencesFeatures Glutathione resin also available
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IBS™ Buffer
G-BiosciencesThe expression of recombinant proteins is a popular and routinely used technique in protein studies. The expression of recombinant proteins often has one drawback and that is the recombinant proteins aggregate and form inclusion bodies, especially when expressed at high levels. The aggregated…
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Potassium Phosphate (Dibasic)
G-BiosciencesFeatures Synonym: Dipotassium hydrogenphosphate CAS#: 7758-11-4 Molecular Formula: HK2O4P Molecular Weight: 174.18
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Zymogram Development Buffer
G-BiosciencesZymography is an electrophoretic technique, based on SDS-PAGE, that includes an enzyme substrate copolymerized with the polyacrylamide gel. Samples are prepared in Zymogram Sample Buffer without boiling to preserve the structure and activity of the enzyme. Following electrophoresis, the SDS is…
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Tris-Acetate SDS Buffer Kit
G-BiosciencesTris-Acetate SDS Buffer Kit is a complete kit to resolve high molecular weight proteins (36-400 kDa) under denaturing conditions on Tris/Acetate gels. Features Tris-Acetate SDS Buffer Kit includes Tris-Acetate/SDS Running Buffer [20X], DTT [0.5 M,10X], Protein Antioxidant [200X] and LDS…
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Antigen Retrieval Solution, Citrate (pH 6.0) [100X]
G-BiosciencesSolution developed to unmask antigens in paraffin embeddedtissue sections. Optimized to work in conjunction with a high heat/pressure treatment.
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JAW™ Tris-Glycine Buffer Packs
G-BiosciencesTris-Glycine powder pouches are dry-blend, pre-measured powder packs used to make standard transfer buffer or running buffer for native PAGE. Features : Dissolve contents of packet in deionized water and 100 methanol to make up to final volume of 500 ml. The resulting buffer makes 25mM…
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His Elution Buffer
G-BiosciencesHis Elution Buffer for Immobilized Metal Affinity Chromatography. Suitable for our Immobilized Cobalt , Copper , Nickel , and Zinc chelating resins Features 250mM Imidazole, 300mM NaCl, 50mM sodium phosphate (pH8.0) Immobilized Cobalt , Copper , Nickel , and Zinc …
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Tris-HEPES/ SDS Running Buffer [20X]
G-BiosciencesTris-HEPES/ SDS Running Buffer [20X] is made to be used with Tris-HEPES gels. Features Compatible with Tris-HEPES gels Results in excellent protein separation and high-resolution protein bands. Run time for this gel system is less, around 45 minutes 1 X concentration: 0.1 M Tris…