A hands on lab activity to study immunoaffinity chromatography and use a specific antibody to purify antigenic proteins from complex samples. This technique involves performing a chromatography procedure using antigen or antibody immobilized on a chromatographic resin.   The solution…

An interesting hands-on lab activity that teaches students the skills required for the isolation of bacteria from test samples. This kit teaches aseptic handling techniques and cultivation of bacteria.   Using bacterial culture techniques, students discover and isolate the bacteria…

Ion exchange chromatography is used to separate charged molecules from complex biological samples. The charged molecules bind to a solid support carrying an opposite charge to the molecule. Proteins contain regions of charged groups on the surface which interact with the ion exchange groups…

Water pollution is a major problem throughout the world, raising severe concerns about health and sanitization issues.  A common problem in polluted water is the presence of bacteria, particularly coliform bacteria from fecal matter, which can directly result in disease, as well as act as a…

This Biotechnology Basics™ kit by Ellyn Daugherty is designed to teach students about RFLP (restriction fragment length polymorphisms) and its use in crime scene investigations. Students will use restriction enzyme digestions of victim, suspect and crime scene DNA and then visualize the…

This kit is designed to teach 8 groups of four students the principles of restriction digestion and agarose electrophoresis. The kit contains the reagents to allow students to: Set up restriction digests of the p-Amylase plasmid with two enzymes Set up positive and negative controls for the…

Antibiotics play a crucial role in the manipulation, screening and killing of bacteria in a range of biotechnology processes.  This kit specifically teaches the basic principles of antibiotics, bacterial resistance and susceptibility. Students learn and understand the use of antibiotic…

Size exclusion chromatography (SEC), also called gel filtration chromatography or gel-permeation chromatography (GPC), uses porous particles to separate molecules of different sizes.  It is generally used to separate biological molecules and to determine molecular weights and molecular weight…

Dialysis is a routinely used technique in research laboratories to “change” the solution a biomolecule is dissolved in. Often the buffers used to isolate biomolecules, such as proteins, are not compatible with downstream applications due to high concentrations of reagents, such as…

This Biotechnology Basics™ kit by Ellyn Daugherty is designed to teach the basic principles of a gel box, specifically horizontal agarose gel electrophoresis. The kit is supplied with all the reagents necessary to conduct 4 gel box science experiments, each using a gel box (not supplied). …

This lab activity teaches the use of highly specific matching properties of antigen-antibody for detection of antigenic or antibody proteins in complex samples.   One such immunodetection method is widely known as Dot Blot. The method involves applying small volumes of antigens or test…

The kit utilizes the principle of diffusion of nucleic acids on a nylon membrane to determine their concentration. No spectrophotometers required. Nucleic acid concentration can be measured by measuring the diameter of diff used nucleic acid spots or comparing the color density of the spots with…

Determination of protein concentration is an essential technique in all aspects of protein studies and proteomics.  The Assays for Protein Quantification kit includes three of the most widely used protein assays and allows for a direct comparison of the three assays that teaches students…

Our Bacterial Gram Staining kit is supplied with three strains of bacteria and all the necessary prealiquoted components to carry out the Gram staining to determine Gram positive and negative bacteria. The Gram staining method was first described in 1844 by the Danish bacteriologist Hans…

An important and essential tool in protein analysis is electrophoresis.  Protein electrophoresis allows researchers to separate complex protein samples by their molecular size and/or net charge on polyacrylamide gels.  The proteins can be subsequently visualized with various protein…

Agarose gel electrophoresis is a routinely used tool for separating nucleic acids.    Nucleic acids are negatively charged molecules, which when loaded onto the solid agarose matrix, migrate in the presence of an electric field, separating the nucleic acids by size.   …

Southern blot analysis, named after its inventor Edwin M. Southern, is a common research technique for enhancing the results of agarose electrophoresis. DNA is resolved on an agarose gel and is subsequently transferred to a nylon membrane.    The resulting membrane can be probed for…

A PCR Based Laboratory Research Experiment.  A major challenge for molecular biologists and genetic engineers is to easily detect and analyze genetic mutations that occur naturally, causing diseases, or during genetic engineering or cloning, whether deliberate or accidental.   …

RNA isolation is an important tool in understanding gene and protein expression regulation. The RNA Isolation kit is a safe RNA isolation kit as it does not utilize toxic phenol or chloroform. Each kit is provided with an extraction buffer for the lysis of biological samples and inhibition of…

A comprehensive program that prepares adults and teenagers for a variety of post-secondary options including community or career college biotechnology certificate programs, four-year biotechnology degree programs, and industry workplaces. The vast text and laboratory curriculum was development…

Determination of protein concentration is an essential technique in all aspects of protein studies and proteomics.    The Assays for Protein Quantification kit includes three of the most widely used protein assays and allows for a direct comparison of the three assays that teaches…

Today’s media routinely covers forensic stories of criminals captured and innocents released using the powerful DNA fingerprinting technique. This kit allows students to carry out their own criminal investigation by comparing DNA samples collected from suspects to DNA collected at a…

Can E. coli cells be transformed into cells that have two new characteristics (ampicillin-resistance and green fluorescent protein production)? This Biotechnology Basics™ kit by Ellyn Daugherty is designed to teach students the basic principles of plasmids, antibiotic resistance,…

Membranes play a crucial role in cells by enclosing specific compartments and regulating the entry and exit of metabolites and other crucial components in and out of a cell.  They also play a key role in signaling from plasma membrane receptors to the cell nucleus.     The…

This kit is designed to teach 8 groups of four students the principles of ELISA (Enzyme Linked ImmunoSorbent Assays).   The kit contains the reagents to allow students to: Conduct a serial dilution of a stock amylase solution to create standards of known concentration Bind known…

Molecular cloning is a commonly used technique for the generation of recombinant proteins.  The gene for a protein of interest is cloned into a vector, or plasmid, in frame with the gene for a protein tag.  These tags are used to purify expressed protein by established…

Enzymic proteins are functional molecular engines of life and as such carry out cellular reactions in cells. Enzymes convert a target molecule (substrate) into a different molecule (product). Enzymes have a number of distinct advantages over conventional chemical catalysts. The most distinctive…

Our Genomic DNA Spooling kit allows for 8 groups of 2-4 students to spool salmon testes DNA onto a spooling rod.  This lab module allows students to precipitate the DNA from solution before spooling (winding) onto a rod.  The recovered DNA is returned to solution and the students then use…

Our Genomic DNA Isolation kits is supplied with dried E.coli and all the ncessary prealiquoted components to isolate bacterial genomic DNA from the bacteria and use G-Biosicences Nucleic Acid Assay to determine the amount of DNA extracted. In addition, a class demonstration is include to…

This kit is designed to teach 8 groups of four students the principles of the polymerase chain reaction (PCR)   The kit contains the reagents to allow students to: Set up multiple PCR reactions using pAmylase plasmid DNA and bacterial genomic DNA templates Set up a PCR negative…

This kit is designed to teach 8 groups of four students the principles of plasmid DNA purification by minipreps. The kit contains the reagents to allow students to: Collect p-Amylase-transformed bacteria from an overnight culture Resuspend bacteria, lyse and isolate the p-Amylase…

The aim of proteomics is to identify and characterize all the proteins in an organism.  The major restrictions in protein identification are the large number of proteins and the huge differences in abundance.  These restrictions mean low abundance, critical proteins are often masked and…