Antibiotics play a crucial role in the manipulation, screening and killing of bacteria in a range of biotechnology processes.  This kit specifically teaches the basic principles of antibiotics, bacterial resistance and susceptibility. Students learn and understand the use of antibiotic…

A comprehensive program that prepares adults and teenagers for a variety of post-secondary options including community or career college biotechnology certificate programs, four-year biotechnology degree programs, and industry workplaces. The vast text and laboratory curriculum was development…

Agarose gel electrophoresis is a routinely used tool for separating nucleic acids.    Nucleic acids are negatively charged molecules, which when loaded onto the solid agarose matrix, migrate in the presence of an electric field, separating the nucleic acids by size.   …

Determination of protein concentration is an essential technique in all aspects of protein studies and proteomics.  The Assays for Protein Quantification kit includes three of the most widely used protein assays and allows for a direct comparison of the three assays that teaches students…

Our Bacterial Gram Staining kit is supplied with three strains of bacteria and all the necessary prealiquoted components to carry out the Gram staining to determine Gram positive and negative bacteria. The Gram staining method was first described in 1844 by the Danish bacteriologist Hans…

This lab activity teaches the use of highly specific matching properties of antigen-antibody for detection of antigenic or antibody proteins in complex samples.   One such immunodetection method is widely known as Dot Blot. The method involves applying small volumes of antigens or test…

Determination of protein concentration is an essential technique in all aspects of protein studies and proteomics.    The Assays for Protein Quantification kit includes three of the most widely used protein assays and allows for a direct comparison of the three assays that teaches…

Water pollution is a major problem throughout the world, raising severe concerns about health and sanitization issues.  A common problem in polluted water is the presence of bacteria, particularly coliform bacteria from fecal matter, which can directly result in disease, as well as act as a…

This Biotechnology Basics™ kit by Ellyn Daugherty is designed to teach the basic principles of a gel box, specifically horizontal agarose gel electrophoresis. The kit is supplied with all the reagents necessary to conduct 4 gel box science experiments, each using a gel box (not supplied). …

Nucleic acids (DNA and RNA) and proteins are routinely resolved by electrophoresis, which separates molecules based on size and/or charge. Following electrophoresis, the molecules need to be extracted from the electrophoresis medium for use in downstream applications and experiments.…

An important and essential tool in protein analysis is electrophoresis.  Protein electrophoresis allows researchers to separate complex protein samples by their molecular size and/or net charge on polyacrylamide gels.  The proteins can be subsequently visualized with various protein…

Dialysis is a routinely used technique in research laboratories to “change” the solution a biomolecule is dissolved in. Often the buffers used to isolate biomolecules, such as proteins, are not compatible with downstream applications due to high concentrations of reagents, such as…

This Biotechnology Basics™ by Ellyn Daugherty is designed to teach students the basic principles of measuring small volumes (<500µl) using micropipettes. The kit is supplied with all the reagents to allow 8 groups of 4 students to learn about P-1000, P-200 or P-100 and P-20 or P-10…

Enzymic proteins are functional molecular engines of life and as such carry out cellular reactions in cells. Enzymes convert a target molecule (substrate) into a different molecule (product). Enzymes have a number of distinct advantages over conventional chemical catalysts. The most distinctive…

The protein make-up of an organism is unique to that individual species, however many essential proteins are highly conserved.  This level of conservation can be seen when protein fingerprinting is used to compare and contrast different organisms. The Conservation of Genetic Information…

Determination of protein concentration is an essential technique in all aspects of protein studies and proteomics.    The Assays for Protein Quantification kit includes three of the most widely used protein assays and allows for a direct comparison of the three assays that teaches…

This kit is designed to teach 8 groups of four students the principles of cheese making.   The kit contains the reagents to allow students to: Set up a controlled experiment & collect numerical data Determine which curdling agent tested produces cheese at the fastest rate …

An interesting hands-on lab activity that teaches students the skills required for the isolation of bacteria from test samples. This kit teaches aseptic handling techniques and cultivation of bacteria.   Using bacterial culture techniques, students discover and isolate the bacteria…

Zymography is an electrophoretic technique that includes a substrate copolymerised with the polyacrylamide gel for the detection of enzymes and their activity. Samples are prepared without denaturing the active enzymes present in the samples. Following electrophoresis, the gel is placed in an…

Molecular biology and genetic engineering are important skills used in the scientific world that allow for the modification and manipulation of DNA to generate proteins that have been used as drugs or novel food sources.  The techniques of molecular biology and genetic engineering are crucial…

The aim of proteomics is to identify and characterize all the proteins in an organism.  The major restrictions in protein identification are the large number of proteins and the huge differences in abundance.  These restrictions mean low abundance, critical proteins are often masked and…

Our Genomic DNA Spooling kit allows for 8 groups of 2-4 students to spool salmon testes DNA onto a spooling rod.  This lab module allows students to precipitate the DNA from solution before spooling (winding) onto a rod.  The recovered DNA is returned to solution and the students then use…

Determination of protein concentration is an essential technique in all aspects of protein studies and proteomics.    The Assays for Protein Quantification kit includes three of the most widely used protein assays and allows for a direct comparison of the three assays that teaches…

This kit is designed to teach 8 groups of four students the principles of the polymerase chain reaction (PCR)   The kit contains the reagents to allow students to: Set up multiple PCR reactions using pAmylase plasmid DNA and bacterial genomic DNA templates Set up a PCR negative…

This kit is designed to teach 8 groups of four students the principles of restriction digestion and agarose electrophoresis. The kit contains the reagents to allow students to: Set up restriction digests of the p-Amylase plasmid with two enzymes Set up positive and negative controls for the…

Lab activity that teaches use of specific lock-key matching properties of antigen-antibody for detection and characterization of antibody or antigenic proteins in complex samples. One such widely used method is known as Western Blot analysis. Western Blot analysis involves electrophoresis…

This kit is designed to teach 8 groups of four students the principles of enzyme assays.   The kit contains the reagents to allow students to test for the presence and activity of 2 different types of amylase by: Visualizing the breakdown of the starch substrate by the amylase…

This kit is designed to teach 8 groups of four students the principles of plasmid DNA purification by minipreps. The kit contains the reagents to allow students to: Collect p-Amylase-transformed bacteria from an overnight culture Resuspend bacteria, lyse and isolate the p-Amylase…

This kit is designed to teach 8 groups of four students the principles of protein concentration estimation assays. The kit contains the reagents to allow students to: Conduct a serial dilution of a stock amylase solution to create standards of known concentration Use a Bradford protein assay…

This kit is designed to teach 8 groups of four students the principles of bacterial transformation.   The kit contains the reagents to allow students to: Generate E.coli comptent cells Transform competent cells with the p-Amylase plasmid Grow transformed colonies on selection…

A PCR Based Laboratory Research Experiment.  A major challenge for molecular biologists and genetic engineers is to easily detect and analyze genetic mutations that occur naturally, causing diseases, or during genetic engineering or cloning, whether deliberate or accidental.   …

Restriction enzymes are DNA-cutting enzymes found in bacteria and as they cut within the molecule, are often called restriction endonucleases. A restriction enzyme recognizes and cuts DNA only at a particular unique sequence of nucleotides, allowing for restriction sites to be mapped.   …