A high salt RAB (Reassembly) buffer for the lysis of mammalian cells, including CHO1,2, COS3, NT2N4,5 and HEK29310; C. elegans6,10 and brain tissue7-9.
Designed for the extraction of soluble proteins, does not extract detergent extractable insoluble proteins.
Features
- Composition: 100mM MES, 1mM EGTA, 0.5mM MgSO4, 0.75M NaCl, 20mM NaF
Applications
- Designed for the extraction of soluble proteins, does not extract detergent extractable insoluble proteins.