Substrates

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  • 2,3,5-Triphenyltetrazolium chloride Tetrazolium Red   Triphenyl tetrazolium chloride, TTC, or Tetrazolium chloride   MOLECULAR BIOLOGY GRADE   Off white to yellowish powder   Forms colorless to yellowish (practically clear) solution when dissolved 5% in ethanol or…

  • (X-glu, X-glcA, BCIG)  5-Bromo-4-chloro-3-indoxyl-beta-D-glucuronide cyclohexylammonium salt   MOLECULAR BIOLOGY GRADE   Purity: greater than 99% (HPLC)   Thin Layer Chromatogram: single spot    Biological Activity: indigo-blue assay   Elemental…

  • 3,3’,5,5’-Tetramethylbenzidine, free base, TMB,    MOLECULAR BIOLOGY GRADE   TMB is an excellent colorimetric substrate for detection of horseradish peroxidase labelled probes and is used with peroxidase and peroxidase coupled systems, particularly in…

  • Phenyl-β-D- galactopyranoside Phenyl-β-D-galactoside Ph-β-D-Gal MOLECULAR BIOLOGY GRADE White to off white crystalline powder Phenyl-β-D-galactopyranoside is a noninducing β-galactosidase substrate. It is used in selection of mutants of lacZ plasmid-based…

  • 4-Methylumbelliferyl-β- D-galactopyranoside 4-MU-β-D-Gal   MOLECULAR BIOLOGY GRADE   White to off-white powder   Fluorogenic substrate for β-D-galactosidase, yielding a blue fluorescent solution upon cleavage.    Store at -15°C…

  • Resazurin, sodium salt

    G-Biosciences

    Resazurin, sodium salt Diazoresorcinol   MOLECULAR BIOLOGY GRADE Black crystalline powder   Transition of pH Indicator: 3.8 - 6.5 orange to violet. Used in Resazurin Microtiter Assay for rapid detection of multidrug-resistant Mycobacterium tuberculosis. Used in…

  • 1-O-Methyl-α- D- galactopyranoside 1-O-Methyl-galactoside 1-OMe-alpha-D-Gal   MOLECULAR BIOLOGY GRADE White to off white crystalline powder   A gratuitous α-galactoside inducer   Store at 2-8°C and protect from light. …

  • Magenta-gal is used as an alternative to X-gal that produces a red color. Magenta-Gal is hydrolyzed by β-galactosidase to produce a red precipitate. This precipitate is insoluble in alcohol and xylenes, thus making it suitable for immunoblotting and immunocytochemical assays. Bacterial…

  • 4-Nitrophenyl phosphate, disodium salt hexahydrate PNPP, p-nitrophenyl phosphate, para nitrophenyl phosphate, PNP-phos · 2 Na   MOLECULAR BIOLOGY GRADE   Off white to light yellow crystalline powder   Chromogenic substrate yielding a soluble yellow chromophor for the…

  • 4-Nitrophenyl-β-D- glucopyranoside p-Nitrophenyl-β-D-glucoside PNP-β-D-Glc   MOLECULAR BIOLOGY GRADE   White to off white powder   Chromogenic β-D-glucosidase substrate, yielding a yellow solution upon cleavage.   Store at -15°C and…

  • 5-Bromo-4-chloro-3-indoxyl-2-acetamido-2-deoxy-β-D-galactopyranoside; 5-Bromo-4-chloro-3-indoxyl-N-acetyl-β-D-galactosaminide X-β-D-GalNAc MOLECULAR BIOLOGY GRADE Used as a chromogenic substrate for N-Acetyl-β-D-galactosaminidase, yielding a blue precipitate. It is also…

  • 4-Methylumbelliferyl-N- acetyl-β-D- galactoside 4-Methylumbelliferyl-2-acetamido-2-deoxy-beta-D-galactopyranoside 4-MU-beta-D-GalNAc   MOLECULAR BIOLOGY GRADE   White to off white powder   Fluorogenic substrate for N-acetyl-beta-D-galactosaminidase.…

  • 5,5'-dithio-bis-(2-nitrobenzoic acid) (DTNB)   Ellman's reagent is a versatile, water-soluble compound for quantifying free sulfhydryl groups in solution. It reacts with a free sulfhydryl group to yield a mixed disulfide and 2-nitro-5-thiobenzoic acid (NTB), a measurable yellow…

  • X-Sulfate potassium salt; 5-Bromo-4-chloro-3-indoxyl sulfate, potassium salt MOLECULAR BIOLOGY GRADE X-Sulfate K is a slightly off-white to faint-yellowish powder that is used as a chromogenic substrate for aryl-sulfatase. X-Sulfate K yields a blue precipitate when cleaved. Store at…

  • 4-Nitrophenyl-α-D- maltohexaoside p-Nitrophenyl-alpha-D-maltohexaoside PNP-alpha-D-maltohexaoside   MOLECULAR BIOLOGY GRADE   Off-white to yellow crystalline powder   Chromogenic substrate for α-amylase, yielding a yellow solution upon cleavage. …

  • Tested and Certified for in vivo imaging Luciferin is a common bioluminescent reporter used for in vivo imaging of the expression of luciferase. This water soluble substrate for the Firefly luciferase enzyme utilizes ATP and Mg2+ as co-factors to emit a characteristic yellow-green emission in…

  • 3,3′,5,5′-Tetramethylbenzidine dihydrochloride; TMB·HCl; TMB dihydrochloride   MOLECULAR BIOLOGY GRADE   TMB is an excellent colorimetric substrate for detection of horseradish peroxidase labelled probes and is used with peroxidase and peroxidase coupled systems,…

  • MUG

    G-Biosciences

    Mug; 4-Methylumbelliferyl beta-D-glucuronide Hydrated form of MUG is CAS # 881005-91-0. MOLECULAR BIOLOGY GRADE 4-methylumbelliferyl-beta-D-glucuronide (MUG) is commonly used for the detection of Escherichia coli through its ability to detect activity of beta-glucuronidase…

  • 4-Aminophenyl phosphate monosodium salt hydrate p-aminophenyl phosphate (pAPP)    MOLECULAR BIOLOGY GRADE   Store at -15°C, protect from light.   Features CAS#:  108084-47-5 Molecular Formula:  C6H7NO4PNa …

  • PNPG (Gus); 4-Nitrophenyl-β-D-glucuronic acid; p-Nitrophenyl-β-D-glucuronide; PNP-β-D-GlcA   MOLECULAR BIOLOGY GRADE   Slightly off-white to yellow crystalline powder.   PNPG (Gus) is a chromogenic β-glucuronidase substrate yielding a yellow…

  • Coelenterazine 400 a

    G-Biosciences

    Coelenterazine 400A is a derivative of coelenterazine and is a good substrate for Renilla reniformis luciferase (RLuc), but does not oxidize well with Gaussia (GLuc). It is the preferred substrate for BRET (Bioluminescence Resonance Energy Transfer) since its emission maximum of 400nm has minimal…

  • 4-Nitrophenyl-β-D- xylopyranoside p-Nitrophenyl-β-D-xylopyranoside PNP-β-D-Xyl   MOLECULAR BIOLOGY GRADE   White to off white powder   Chromogenic β-xylosidase substrate, yielding a yellow solution upon cleavage.   Store at -15°C and…

  • 4-Nitrophenyl-N-acetyl-β- D-glucosaminide p-Nitrophenyl-2-acetamido-2-deoxy-β-D-glucopyranoside PNP-β-D-GlcNAc   MOLECULAR BIOLOGY GRADE   Off white crystalline powder   Chromogenic β-Glucosaminidase substrate yielding a yellow solution upon…

  • Tested and Certified for in vivo imaging. Luciferin is a common bioluminescent reporter used for in vivo imaging of the expression of luciferase. This water soluble substrate for the Firefly luciferase enzyme utilizes ATP and Mg2+ as co-factors to emit a characteristic yellow-green emission in…

  • 4-Methylumbelliferyl caprylate Octanoic acid 4-methylumbelliferyl ester 4-MU-caprylate   MOLECULAR BIOLOGY GRADE   White to off white crystalline powder   Fluorogenic substrate for esterase with C8 activity yielding a blue fluorescent solution upon cleavage. Used for the…

  • 4-Nitrophenyl-α-D- galactopyranoside p-Nitrophenyl-alpha-D-galactoside PNP-alpha-D-Gal PNPG   MOLECULAR BIOLOGY GRADE   Off white crystalline powder   Chromogenic substrate yielding a soluble chromophor for the colorimetric assay of alpha-D-galactosidase upon…

  • Nitrotetrazolium blue chloride; NBT; Nitro blue tetrazolium chloride; Nitro-TB MOLECULAR BIOLOGY GRADE NBT is used in combination with 5-bromo-4-chloro-3-indoxyl phosphate (BCIP) to detect alkaline phosphatase activity in blotting techniques and in situ hybridization chemistry. BCIP acts…

  • L-Lysine 7-amido-4- methylcoumarin acetate salt H-Lys-AMC · AcOH   MOLECULAR BIOLOGY GRADE Off white to white crystalline powder   Forms a colorless, clear solution when dissolved 5% in water.   Substrate for aminopeptidase b yielding a blue…

  • 2-Naphthyl-β- D-galactopyranoside β-Naphthyl-β-galactoside; β-Nap-β-D-Gal   MOLECULAR BIOLOGY GRADE   White to off-white crystalline powder   2-Naphthyl-beta-D-galactopyranoside is a substrate for beta-galactosidase. 2-naphthol is released upon…

  • 4-Nitrophenyl-α-D- glucopyranoside p-Nitrophenyl-alpha-D-glucoside PNP-alpha-D-Glc   MOLECULAR BIOLOGY GRADE   Off white crystalline powder   Chromogenic substrate for alpha-D-glucosidase yielding a yellow solution upon cleavage. Also used for the detection of…

  • 4-Aminophenyl-β-D-gal   MOLECULAR BIOLOGY GRADE   4-Aminophenyl-β-D-galactopyranoside is a substrate for β-galactosidase. It is also a weak β-D-galactosidase inducer and can be used in affinity chromatography for isolation of galactose-binding. The 4-aminophenyl…

  • X-pal 5-Bromo-4-chloro-3-indoxyl palmitate MOLECULAR BIOLOGY GRADE X-pal is an off white to light brownish powder that is used as a chromogenic substrate for carboxylesterase. X-pal yields a blue precipitate when cleaved. Store at -15°C and protect from light. Features …

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