Cayman Chemical

A fluorescently-tagged cholesterol with the hydrophilic NBD fluorophore attached to carbon 3, at the hydrophilic end of cholesterol, separated by a 6-carbon spacer; allows the cholesterol to properly orient in membrane bi-layers while the fluorescent tag is presented outside of the bi-layer.

A pentacyclic triperpene isolated from birch tree bark that inhibits the SREBP-driven pathway of cholesterol and fatty acid biosynthesis; 15-30 mg/kg/day decreases lipid levels and increases insulin sensitivity in mice fed a western-type diet and reduces the size and improves the stability of...

An endogenous CB receptor ligand that is 10-100 times less potent than 2-AG in ligand binding affinity and agonist activity at the CB1 receptor; 2-AG undergoes rapid isomerization to 1-AG, which makes it a frequent contaminant in synthetic 2-AG preparations, markedly reducing their cannabinergic...

Analog of PEA which incorporates isopropyl amide in place of the native ethanolamide; inhibits the re-uptake of AEA, as well as FAAH (IC₅₀ = 10 µM).

Cayman’s cGMP Assay is a competitive ELISA that can be used for quantification of cGMP directly obtained from cell lysates, tissue homogenates, plasma or urine. Since the antibody used in this assay was prepared against a cGMP-carrier protein conjugate, antibody binding is increased if an...

An agonist of GPR81 (EC₅₀ = 16 µM) that is inactive against the related GPR109a (HCAR2) receptor; stimulates lipolysis (increased serum free fatty acids) in mice fed high fat chow for 10 weeks; blocks basal ghrelin secretion by primary gastric mucosal cells.

A potent and selective inhibitor of CECR2 (IC₅₀ = 0.047 µM by Alpha screen, K_d = 0.80 µM by ITC); shows robust activity in cells, by FRAP assay, due to its slow off-rate.

One of a number of lipophilic, long acyl side-chain bearing AHLs produced by the LuxI AHL synthase homolog SinI involved in quorum-sensing signaling in Sinorhizobium meliloti, a nitrogen-fixing bacterial symbiont of certain legumes; the most abundant AHL produced by the proteobacterium Rhodobacter...

A synthetic analog of natural PtdIns containing C8:0 fatty acids at the sn-1 and sn-2 positions; contains the same inositol and DAG stereochemistry as the natural compound.

INDIGO’s panel of LXR Assays utilizes non-human mammalian cells engineered to express human estrogen receptors alpha (NR1H3) and beta (NR1H2), ligand-dependent transcription factors commonly referred to as LXRα and LXRβ. INDIGO’s reporter cells include the luciferase reporter...

An internal standard for the quantification of 25-hydroxy cholesterol by GC- or LC-MS.

A selective inhibitor of sAC with an IC₅₀ value between 3-10 µM; decreases basal cAMP accumulation in sperm at 50 µM; used in diverse research applications including cAMP-mediated signaling events required for mammalian egg fertilization, sAC regulation of Na+ transport in the...

A microtubule-stabilizing agent that inhibits the growth of a variety of cancer cells both in vitro (IC₅₀ values range from 0.97 to 21 nM) and in mice; brain penetrant and reduces neurodegeneration in aged tau transgenic mice; attenuates nigrostriatal degeneration in a mouse model of...

A substrate of S1P lyase, leading to the production of the fluorescent product umbelliferone; intended to be used to monitor or measure S1P lyase activity.

A calcimimetic that allosterically activates the calcium-sensing receptor (CaSR; pEC₅₀ = 7.3 in HEK293T cells expressing CaSR) inhibiting parathyroid hormone secretion.

Peptide sequence: 2-221 - Full-length Source: Recombinant N-terminal His- and SUMOpro-tagged protein expressed in E. coli Mr: 37.6 kDa

A selective sEH inhibitor with IC50 values of 160 and 90 nM for recombinant human and mouse sEH, respectively; at 10 µM, blocks the hydrolysis of cis- and trans-EETs and completely inhibits 14,(15)-EET-induced PPAR activation.

Racemic version of the 9,10-epoxide of linoleic acid generated by neutrophils during the oxidative burst.

A methyl hydrazine derivative that is converted by metabolism into a methylating species which converts DNA to various DNA adducts, including O6-methylguanine.

A specific inhibitor of the proteolytic activity of Omi/HtrA2 (IC₅₀ = 9.5 μM) that is naturally fluorescent, thus enabling the visualization of its entry into cells; has been reported to decrease cerebral infarct size in a rat model of cerebral ischemia and to be protective of...

An internal standard for the quantification of DHEA by GC- or LC-MS.

A crosslinking reagent that contains NHS ester- and maleimide-reactive groups at the opposite ends of a 4.4 Å spacer arm; used for sequential, two-stage conjugation with amine and sulfhydryl functional groups in the preparation of protein-hapten or protein-protein conjugates.

Selective inhibitor of monoacylglycerol hydrolysis; blocks 2-oleoyl glycerol hydrolysis in the cytosolic (IC50 = 4.5 µM) and membrane (IC50 = 19 µM) fractions of rat cerebella.

A product of 5-LO catalyzed oxidation of arachidonic acid; further metabolism by 5-LO gives LTA4, a key intermediate in the formation of the 4-series LTs.